FastQCFastQC Report
Fri 10 Feb 2017
ERR1630903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692195
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT134291.9400602431395777No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG108451.5667550329025781No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT102441.4799297885711395Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC57930.8369028958602707No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC40220.5810501376057325Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC28520.4120226236826328No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG15150.21886896033632142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14740.21294577395098202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.19084217597642283No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA10540.15226923049140778No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT8610.12438691409212721No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT7050.10184991223571391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15550.032.47911
TTATACA17650.028.5099142
CGTCTTC5400.028.09259437
TATACAC18700.027.7005333
GGTATCA3200.026.0156271
CCGTCTT16350.025.23241437
GCCGTCT16550.024.92749236
TGCGTTA400.001930414823.12500237
TTACGGG502.7005974E-422.23
GATTACG502.7005974E-422.21
GTATCAA8950.021.9106161
TGCCGTC20100.020.61691535
ACGGCTA656.897059E-519.92307737
ATGCCGT20900.019.82775134
ATAGCGG500.007032123418.57
TAGTTCG500.007032123418.59
TATGCCG23050.018.05856933
TACACAT29650.017.6576735
GTACTAA852.7205819E-517.4117641
ATACACA30500.017.2262294
Produced by FastQC (version 0.11.5)