##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630903.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692195
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43187685551037	31.0	31.0	34.0	28.0	34.0
2	31.442694616401447	31.0	31.0	34.0	28.0	34.0
3	30.915578702533246	31.0	31.0	34.0	27.0	34.0
4	35.06822788376108	37.0	35.0	37.0	32.0	37.0
5	35.24827685839973	37.0	35.0	37.0	32.0	37.0
6	35.43994250175167	37.0	35.0	37.0	33.0	37.0
7	35.434643416956206	37.0	35.0	37.0	33.0	37.0
8	35.46837813044012	37.0	35.0	37.0	33.0	37.0
9	37.23483700402343	39.0	37.0	39.0	34.0	39.0
10	37.059984541928216	39.0	37.0	39.0	33.0	39.0
11	37.12136753371521	39.0	37.0	39.0	33.0	39.0
12	37.04708066368581	39.0	37.0	39.0	33.0	39.0
13	37.13528702172076	39.0	37.0	39.0	33.0	39.0
14	38.26397474700048	40.0	38.0	41.0	33.0	41.0
15	38.274372106126165	40.0	38.0	41.0	33.0	41.0
16	38.27574310707243	40.0	38.0	41.0	33.0	41.0
17	38.22321022255289	40.0	38.0	41.0	33.0	41.0
18	38.2734229516249	40.0	38.0	41.0	33.0	41.0
19	38.300597375017155	40.0	38.0	41.0	34.0	41.0
20	38.316230253035634	40.0	38.0	41.0	34.0	41.0
21	38.27200138689242	40.0	38.0	41.0	34.0	41.0
22	38.199199647498176	40.0	38.0	41.0	34.0	41.0
23	38.19062547403549	40.0	38.0	41.0	34.0	41.0
24	38.1655212765189	40.0	38.0	41.0	33.0	41.0
25	38.13248723264398	40.0	37.0	41.0	33.0	41.0
26	37.990995312014675	40.0	37.0	41.0	33.0	41.0
27	37.96019040877209	40.0	37.0	41.0	33.0	41.0
28	37.93841764242735	40.0	37.0	41.0	33.0	41.0
29	37.872296101532086	40.0	37.0	41.0	33.0	41.0
30	37.822668467700574	40.0	37.0	41.0	33.0	41.0
31	37.79816959093897	40.0	37.0	41.0	33.0	41.0
32	37.71258532638924	40.0	37.0	41.0	33.0	41.0
33	37.647570410072305	40.0	37.0	41.0	33.0	41.0
34	37.58368667788701	40.0	37.0	41.0	32.0	41.0
35	37.60125542657777	40.0	37.0	41.0	32.0	41.0
36	37.54583029348666	40.0	37.0	41.0	32.0	41.0
37	37.48600755567434	40.0	37.0	41.0	32.0	41.0
38	37.47211118254249	39.0	37.0	41.0	32.0	41.0
39	37.38682307731203	39.0	37.0	41.0	32.0	41.0
40	37.28977672476687	39.0	36.0	41.0	31.0	41.0
41	37.2643518083777	39.0	36.0	41.0	31.0	41.0
42	37.228471745678604	39.0	36.0	41.0	31.0	41.0
43	36.56387867580667	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	17.0
11	13.0
12	9.0
13	14.0
14	8.0
15	6.0
16	9.0
17	15.0
18	24.0
19	30.0
20	65.0
21	113.0
22	248.0
23	458.0
24	875.0
25	1565.0
26	2605.0
27	3855.0
28	5825.0
29	8396.0
30	11473.0
31	15336.0
32	20109.0
33	25613.0
34	34310.0
35	46303.0
36	64208.0
37	96207.0
38	169320.0
39	185157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.04230744226699	22.82593777764936	15.044604482840818	25.08715029724283
2	13.482905828559872	21.672650048035596	41.30425674845961	23.54018737494492
3	14.451707972464407	28.322654743244318	34.396810147429555	22.828827136861722
4	11.498060517628703	19.571941432688767	37.48726876097054	31.442729288711995
5	12.421499721899176	38.04072551809822	36.028575762610245	13.509198997392351
6	27.773387556974548	40.00462297473978	17.864908010026078	14.357081458259593
7	24.212396795700634	33.54069301280708	22.827237989294925	19.419672202197358
8	24.371600488301706	35.86850526224546	22.21108213725901	17.54881211219382
9	23.43530363553623	15.043737675077109	19.7853206105216	41.73563807886506
10	13.893772708557558	29.060597086081234	33.94390309089202	23.10172711446919
11	31.14844805293306	22.86075455615831	25.583108806044542	20.407688584864093
12	20.076279083206323	26.334342201258316	29.724716301042335	23.864662414493026
13	28.65464211674456	20.61109947341428	26.81542051011637	23.91883789972479
14	21.31234695425422	22.56315055728516	26.321195616842076	29.803306871618545
15	23.467231054832816	29.181661237079147	22.59377776493618	24.757329943151856
16	24.853112201041615	27.70722123101149	23.66760811621003	23.772058451736868
17	20.671198145031386	31.08170385512753	25.256755683008404	22.990342316832685
18	23.319440331120568	26.60146346044106	25.755170147140618	24.323926061297758
19	22.67771365005526	29.351122154884102	25.40295725915385	22.56820693590679
20	21.67395025968116	26.280455651947786	26.051473934368207	25.994120154002847
21	23.537875887575034	25.963492946351824	27.124581945838962	23.374049220234184
22	21.298622497995506	30.020008812545594	25.676724044525024	23.00464464493387
23	23.98644890529403	25.64797492036204	26.128908761259474	24.236667413084465
24	22.72842190423219	28.232940139700517	25.961325926942553	23.07731202912474
25	21.62974306373204	28.0036694861997	27.37364470994445	22.99294274012381
26	23.122530500798185	29.53387412506591	26.273376721877504	21.070218652258397
27	21.132917747166623	29.13225319454778	26.36179111377574	23.373037944509857
28	21.489175738050694	26.141910877715098	28.797087525913938	23.571825858320274
29	21.059816959093897	27.99615715224756	26.237259731722993	24.706766156935544
30	22.45797788195523	25.638006631079392	28.617947254747577	23.2860682322178
31	21.896719854954167	26.06563179450877	27.607393870224435	24.43025448031263
32	22.387621985134246	26.069965833327313	28.49601629598596	23.04639588555248
33	22.74662486727006	27.5246137287903	28.341724514045897	21.387036889893743
34	21.180158770288717	28.21054760580472	26.57199199647498	24.037301627431575
35	21.302089729050337	25.784641611106697	32.110315734727926	20.802952925115033
36	22.84572988825403	27.97867652901278	25.52416587811238	23.651427704620808
37	21.99568040797752	27.115624932280642	27.926668063190284	22.96202659655155
38	21.94208279458823	25.420148946467396	28.428260822456096	24.209507436488273
39	23.930973208416702	25.806022869278166	26.78956074516574	23.473443177139387
40	20.984260215690664	25.199401902643043	31.719096497374295	22.097241384291998
41	22.29400674665376	26.391118109781203	26.39501874471789	24.919856398847145
42	22.72712169258663	25.18567744638433	29.992126496146316	22.095074364882727
43	22.779996966172828	24.882439197047074	29.606830445178023	22.73073339160208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	6.5
2	10.0
3	52.5
4	95.0
5	95.0
6	119.5
7	144.0
8	155.0
9	166.0
10	263.0
11	360.0
12	360.0
13	547.0
14	734.0
15	1597.5
16	2461.0
17	2998.5
18	3536.0
19	3536.0
20	3960.5
21	4385.0
22	5649.5
23	6914.0
24	9344.0
25	11774.0
26	11774.0
27	14502.0
28	17230.0
29	20809.5
30	24389.0
31	27648.5
32	30908.0
33	30908.0
34	34346.0
35	37784.0
36	40163.0
37	42542.0
38	44661.5
39	46781.0
40	46781.0
41	48276.5
42	49772.0
43	50034.5
44	50297.0
45	49990.5
46	49684.0
47	49684.0
48	50005.5
49	50327.0
50	60229.5
51	70132.0
52	55854.5
53	41577.0
54	41577.0
55	46730.0
56	51883.0
57	43820.5
58	35758.0
59	28223.5
60	20689.0
61	20689.0
62	18045.0
63	15401.0
64	12789.0
65	10177.0
66	8451.0
67	6725.0
68	6725.0
69	5434.5
70	4144.0
71	3264.5
72	2385.0
73	1902.0
74	1419.0
75	1419.0
76	1089.5
77	760.0
78	582.0
79	404.0
80	314.5
81	225.0
82	225.0
83	182.5
84	140.0
85	97.5
86	55.0
87	36.0
88	17.0
89	17.0
90	12.5
91	8.0
92	4.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	692195.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.62568361558001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31642475848307	50.72805948531974
2	10.859705017520838	13.384734911365317
3	2.966106608902388	5.483650423508983
4	1.2728496877596631	3.137609285922672
5	0.656579561089779	2.023108215008755
6	0.40659601990474814	1.5034054609202445
7	0.2650813154597879	1.1435072095248653
8	0.18595933121665273	0.9167896728737832
9	0.14641641666688365	0.8120710592676276
>10	0.8610593598189686	9.59396320078756
>50	0.04425541077283009	1.8555518612228972
>100	0.015688349766846653	1.4500636194396592
>500	9.366178964468536E-4	0.38470192150390065
>1k	0.0014049268446702805	1.765934776956015
>5k	2.341544741117134E-4	0.8359258181815815
>10k+	7.024634223351402E-4	4.980923078196415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	13429	1.9400602431395777	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	10845	1.5667550329025781	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	10244	1.4799297885711395	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	5793	0.8369028958602707	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	4022	0.5810501376057325	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	2852	0.4120226236826328	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1515	0.21886896033632142	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1474	0.21294577395098202	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.19084217597642283	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1054	0.15226923049140778	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	861	0.12438691409212721	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	705	0.10184991223571391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4446796061803394E-4	0.0
11	0.0	0.0	0.0	1.4446796061803394E-4	0.0
12	0.0	0.0	0.0	1.4446796061803394E-4	0.0
13	0.0	0.0	0.0	1.4446796061803394E-4	0.0
14	0.0	0.0	0.0	1.4446796061803394E-4	0.0
15	0.0	0.0	0.0	1.4446796061803394E-4	0.0
16	0.0	0.0	0.0	1.4446796061803394E-4	0.0
17	0.0	0.0	0.0	1.4446796061803394E-4	0.0
18	0.0	0.0	0.0	1.4446796061803394E-4	0.0
19	0.0	0.0	0.0	1.4446796061803394E-4	0.0
20	0.0	0.0	0.0	1.4446796061803394E-4	0.0
21	0.0	0.0	0.0	1.4446796061803394E-4	0.0
22	0.0	0.0	0.0	2.889359212360679E-4	0.0
23	0.0	0.0	0.0	5.778718424721358E-4	0.0
24	0.0	0.0	0.0	8.668077637082036E-4	0.0
25	0.0	0.0	0.0	8.668077637082036E-4	0.0
26	0.0	0.0	0.0	0.0013002116455623053	0.0
27	0.0	0.0	0.0	0.0018780834880344412	0.0
28	0.0	0.0	0.0	0.00476744270039512	0.0
29	0.0	0.0	0.0	0.014591264022421427	0.0
30	0.0	0.0	0.0	0.025570829029392006	0.0
31	0.0	0.0	0.0	0.06833334537233005	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1555	0.0	32.4791	1
TTATACA	1765	0.0	28.509914	2
CGTCTTC	540	0.0	28.092594	37
TATACAC	1870	0.0	27.700533	3
GGTATCA	320	0.0	26.015627	1
CCGTCTT	1635	0.0	25.232414	37
GCCGTCT	1655	0.0	24.927492	36
TGCGTTA	40	0.0019304148	23.125002	37
TTACGGG	50	2.7005974E-4	22.2	3
GATTACG	50	2.7005974E-4	22.2	1
GTATCAA	895	0.0	21.910616	1
TGCCGTC	2010	0.0	20.616915	35
ACGGCTA	65	6.897059E-5	19.923077	37
ATGCCGT	2090	0.0	19.827751	34
ATAGCGG	50	0.0070321234	18.5	7
TAGTTCG	50	0.0070321234	18.5	9
TATGCCG	2305	0.0	18.058569	33
TACACAT	2965	0.0	17.657673	5
GTACTAA	85	2.7205819E-5	17.411764	1
ATACACA	3050	0.0	17.226229	4
>>END_MODULE