FastQCFastQC Report
Fri 10 Feb 2017
ERR1630902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284317
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT134734.738724733308244No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT126194.438355778936891TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG121894.287116141489957No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC64202.2580429590914366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55491.9516947632396233No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC54421.9140607139214327RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50961.7923655637897133No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC35681.2549372707224682No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22060.7758945121114812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20410.7178606977423088No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15100.5310973314996993No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG14000.49240812192025096No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA13030.4582912734729193No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12220.4298019464189619No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12020.42276754467724403No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT10650.3745818927464767No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9360.3292100015123964No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9140.3214721595965067No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8830.310568836896844No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8620.30318271506804023No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8360.29403799280380705No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8220.2891139115846045No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8200.2884104714104327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.25394190287601515No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7120.2504247020051562No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6360.2236939753866283No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6280.22088021468994115No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6210.2184181740803399No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6160.21665957364491042No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5750.2022390500743888No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5730.201535609900217No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5500.19344604789724146No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5440.1913357273747261No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5410.1902805671134684No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5210.18324616537175056No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5140.18078412476214928No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5030.17691520380420445No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4840.1702325221495725No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4730.16636360119162766No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4660.1639015605820264No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4340.1526465177952778No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4300.15123963744693422No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4290.15088791735984833No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.15018447718567657No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4110.14455695579230224No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3890.1368191138764126No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3890.1368191138764126No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3800.13365363309263956No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3750.1318950326572101No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3700.13013643222178062No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3700.13013643222178062No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3620.12732267152509347No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3500.12310203048006274No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3480.12239859030589097No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3450.12134343004463327No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3420.1202882697833756No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3420.1202882697833756No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3420.1202882697833756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3330.11712278899960256No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3300.11606762873834486No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3280.11536418856417309No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3270.1150124684770872No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3200.11255042786748595No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3190.11219870778040004No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3090.1086815069095411No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3050.10727462656119754No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2990.10516430603868217No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT2930.1030539855161668TruSeq Adapter, Index 3 (95% over 23bp)
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2920.10270226542908092No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2890.10164710516782323No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2880.10129538508073734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACA200.001839607337.03
AGCAACG403.816058E-837.02
AATCCAG200.001839607337.026
GTCGAAT200.001839607337.022
TTACTTT251.2312896E-437.03
TCGAATC200.001839607337.023
CTTATAC15600.034.391021
CGTCTTC5400.034.2592637
TTACGGG800.032.3753
TTATACA17300.030.7976882
ATTACGG850.030.4705872
GATTACG850.030.4705871
AGCCCCC250.005489473729.637
CCAGAAT250.005489473729.629
TCCAGAA250.005489473729.628
TCAGCCC250.005489473729.635
AATACAC250.005489473729.64
TATACAC18300.029.316943
GACCGTT405.924656E-527.757
GCAACGC556.233913E-726.909093
Produced by FastQC (version 0.11.5)