##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284317
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05398199896594	31.0	30.0	34.0	28.0	34.0
2	31.036346753799457	31.0	31.0	34.0	28.0	34.0
3	30.435253607768793	31.0	30.0	34.0	26.0	34.0
4	34.65017216698263	35.0	35.0	37.0	32.0	37.0
5	34.88335555031883	35.0	35.0	37.0	32.0	37.0
6	35.12332009693406	36.0	35.0	37.0	32.0	37.0
7	35.11585307948522	36.0	35.0	37.0	32.0	37.0
8	35.14322041946138	37.0	35.0	37.0	32.0	37.0
9	36.91747591596704	39.0	37.0	39.0	33.0	39.0
10	36.740578298167186	39.0	37.0	39.0	32.0	39.0
11	36.79076523739347	39.0	37.0	39.0	32.0	39.0
12	36.67038903758833	38.0	35.0	39.0	32.0	39.0
13	36.77367515836197	39.0	37.0	39.0	32.0	39.0
14	37.52314494033069	39.0	36.0	40.0	32.0	41.0
15	37.503916403169704	39.0	36.0	40.0	32.0	41.0
16	37.54136052364087	39.0	36.0	40.0	32.0	41.0
17	37.546347914475746	39.0	37.0	40.0	32.0	41.0
18	37.76974292778835	39.0	37.0	40.0	33.0	41.0
19	37.63720776457264	39.0	37.0	41.0	32.0	41.0
20	37.48602088513877	39.0	37.0	40.0	32.0	41.0
21	37.52860011888139	39.0	36.0	40.0	32.0	41.0
22	37.299039452442166	39.0	36.0	40.0	32.0	41.0
23	37.39866064990838	39.0	36.0	40.0	32.0	41.0
24	37.281499171699195	39.0	36.0	40.0	32.0	41.0
25	37.069236099142856	39.0	36.0	40.0	31.0	41.0
26	36.95694594413982	39.0	35.0	40.0	31.0	41.0
27	36.608546094676015	38.0	35.0	40.0	30.0	41.0
28	36.74930799072866	39.0	35.0	40.0	31.0	41.0
29	36.7538416626512	39.0	35.0	40.0	31.0	41.0
30	36.213937260170866	38.0	35.0	40.0	30.0	41.0
31	36.25030159997468	38.0	35.0	40.0	30.0	41.0
32	36.072366407917926	38.0	35.0	40.0	30.0	41.0
33	35.861868970198756	38.0	35.0	40.0	30.0	41.0
34	35.79118026709624	38.0	35.0	40.0	29.0	41.0
35	35.72548598923033	38.0	35.0	40.0	29.0	41.0
36	35.55085696599218	38.0	34.0	40.0	28.0	41.0
37	35.461931576374255	38.0	34.0	40.0	27.0	41.0
38	35.339694777308424	38.0	34.0	40.0	27.0	41.0
39	35.08951627936423	38.0	34.0	40.0	26.0	41.0
40	34.712940133723976	38.0	33.0	40.0	24.0	41.0
41	34.59375626501405	38.0	33.0	40.0	24.0	41.0
42	34.37149379038186	37.0	33.0	40.0	23.0	41.0
43	33.35328524147343	36.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	18.0
11	19.0
12	24.0
13	14.0
14	12.0
15	6.0
16	13.0
17	23.0
18	26.0
19	51.0
20	96.0
21	199.0
22	300.0
23	569.0
24	957.0
25	1535.0
26	2407.0
27	3459.0
28	4777.0
29	6389.0
30	7934.0
31	9909.0
32	11966.0
33	15250.0
34	19415.0
35	24830.0
36	33328.0
37	47061.0
38	61140.0
39	32576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.431518340443944	21.58893066541923	18.2486449983645	23.730905995772325
2	12.815273093061618	22.588167432830257	41.799470309548845	22.797089164559278
3	15.236162452473822	30.65873655110317	35.76993285663538	18.33516813978763
4	9.844645237534161	19.94463925829268	30.401980887530467	39.808734616642695
5	13.729041879310769	37.4697960375215	29.68130642909147	19.11985565407626
6	25.896094851873087	38.41205415082461	15.339919878164162	20.351931119138143
7	22.54666446255412	34.17980634292005	16.596967469409147	26.67656172511668
8	28.419334756627286	28.45309988498753	23.114340683110754	20.01322467527443
9	21.899147782228994	11.226905179781722	15.912871900027081	50.96107513796221
10	15.929754464207205	27.15208728285681	27.261472229940527	29.65668602299546
11	30.60035101664691	20.995227158418245	22.409493628590624	25.99492819634422
12	20.043472602763817	27.74930799072866	22.907880991991334	29.29933841451619
13	35.433688453381265	17.289152600794182	24.297175335980615	22.97998360984394
14	23.729147395336895	24.161411382365458	25.160296429689392	26.949144792608248
15	28.000787852995074	25.62069802368483	20.9938202780699	25.384693845250194
16	27.224189900709415	23.11539584337201	19.934791095854276	29.725623160064295
17	19.116690173292486	32.17289152600794	20.986434156241096	27.723984144458473
18	27.850603375809396	16.85864721420105	24.47127677908813	30.819472630901423
19	29.198746469609627	23.200512104446798	26.189781124589807	21.41096030135377
20	21.23474853772374	20.7395266551068	29.57649384313988	28.44923096402959
21	29.928917370399944	23.39642019295364	25.009408512329546	21.665253924316872
22	23.4572677680195	32.75111934917715	25.846502319593974	17.945110563209376
23	26.053665450887564	24.4396219712504	25.750482735819524	23.756229842042508
24	33.5379171839883	23.12735432633293	25.478251388415046	17.856477101263728
25	23.624686529472385	28.70915210838606	27.491848886981785	20.17431247515977
26	30.24757576929976	28.589567278776855	24.447008093079205	16.715848858844176
27	23.115747563459095	28.688048903160908	31.851419366411438	16.34478416696856
28	17.55505298663112	28.283219082925044	30.05659176201212	24.105136168431716
29	31.01151179845032	20.811980993046493	25.791282265921488	22.385224942581697
30	19.883088243052647	25.35514935793498	36.321078233098966	18.4406841659134
31	27.677557092963134	25.743448334077808	23.835366861636835	22.74362771132222
32	30.958753785387437	21.46407003450374	28.695083304902624	18.882092875206197
33	18.30527193238533	30.45157341980958	32.472908760292206	18.770245887512882
34	22.725338266793756	28.49776833604744	27.167562966688592	21.609330430470212
35	24.265168808055797	21.49572484234147	36.94713998811186	17.29196636149087
36	22.854067818667193	26.146871273965328	25.199689079443015	25.799371827924467
37	21.495373122254385	24.73999092562175	29.593024687232912	24.17161126489095
38	20.717016569533303	19.462079298810835	30.794852224805414	29.02605190685045
39	27.438035713657644	20.0937685752171	27.599123513542985	24.869072197582277
40	18.61795108980469	20.439861140909617	39.42676660206741	21.515421167218282
41	23.26558032055769	22.036670336279577	25.894336251437654	28.803413091725083
42	23.08198243509885	19.584126169029638	35.87333856223863	21.46055283363288
43	23.52901866578502	19.88379168322682	32.90482102723369	23.68236862375447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	13.0
2	20.0
3	127.0
4	234.0
5	234.0
6	345.0
7	456.0
8	498.5
9	541.0
10	818.0
11	1095.0
12	1095.0
13	1644.0
14	2193.0
15	5285.5
16	8378.0
17	8379.0
18	8380.0
19	8380.0
20	6856.5
21	5333.0
22	4116.5
23	2900.0
24	2824.0
25	2748.0
26	2748.0
27	2683.0
28	2618.0
29	2603.5
30	2589.0
31	2616.0
32	2643.0
33	2643.0
34	3040.5
35	3438.0
36	3445.0
37	3452.0
38	3847.0
39	4242.0
40	4242.0
41	5280.0
42	6318.0
43	8181.0
44	10044.0
45	13389.5
46	16735.0
47	16735.0
48	22003.5
49	27272.0
50	38582.0
51	49892.0
52	38318.5
53	26745.0
54	26745.0
55	34771.5
56	42798.0
57	35621.0
58	28444.0
59	19293.5
60	10143.0
61	10143.0
62	8241.0
63	6339.0
64	4987.0
65	3635.0
66	3026.0
67	2417.0
68	2417.0
69	1862.0
70	1307.0
71	941.5
72	576.0
73	387.0
74	198.0
75	198.0
76	145.5
77	93.0
78	72.0
79	51.0
80	36.0
81	21.0
82	21.0
83	17.5
84	14.0
85	10.0
86	6.0
87	4.0
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	284317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.09423284573205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86996379773444	18.521228065856068
2	10.11327805675581	4.873433526662141
3	3.8289734905990893	2.767685365278896
4	1.941492467593133	1.8711508632969536
5	1.1867920121452762	1.4297421540041573
6	0.8437463505780683	1.2197652620138788
7	0.6393787224103702	1.0783737870053496
8	0.4875627700572229	0.9397960726935076
9	0.36202265561135116	0.7850392343757143
>10	2.9925259838841525	14.611155857722192
>50	0.38829849351862666	6.660875009232653
>100	0.29195375452528316	13.36395642891561
>500	0.030655144225154734	5.0397971278537685
>1k	0.013137918953637744	5.457640591311811
>5k	0.005839075090505665	7.916164000042207
>10k+	0.004379306317879248	13.464196653735092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	13473	4.738724733308244	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	12619	4.438355778936891	TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	12189	4.287116141489957	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	6420	2.2580429590914366	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5549	1.9516947632396233	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	5442	1.9140607139214327	RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5096	1.7923655637897133	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	3568	1.2549372707224682	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2206	0.7758945121114812	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.7178606977423088	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1510	0.5310973314996993	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1400	0.49240812192025096	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1303	0.4582912734729193	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1222	0.4298019464189619	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1202	0.42276754467724403	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1065	0.3745818927464767	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	936	0.3292100015123964	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	914	0.3214721595965067	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	883	0.310568836896844	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	862	0.30318271506804023	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	836	0.29403799280380705	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	822	0.2891139115846045	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	820	0.2884104714104327	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.25394190287601515	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	712	0.2504247020051562	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	636	0.2236939753866283	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	628	0.22088021468994115	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	621	0.2184181740803399	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	616	0.21665957364491042	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	575	0.2022390500743888	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	573	0.201535609900217	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	550	0.19344604789724146	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	544	0.1913357273747261	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	541	0.1902805671134684	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	521	0.18324616537175056	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	514	0.18078412476214928	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	503	0.17691520380420445	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	484	0.1702325221495725	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	473	0.16636360119162766	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	466	0.1639015605820264	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	434	0.1526465177952778	No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT	430	0.15123963744693422	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	429	0.15088791735984833	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.15018447718567657	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	411	0.14455695579230224	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	389	0.1368191138764126	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	389	0.1368191138764126	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	380	0.13365363309263956	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	375	0.1318950326572101	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	370	0.13013643222178062	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	370	0.13013643222178062	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	362	0.12732267152509347	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	350	0.12310203048006274	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	348	0.12239859030589097	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	345	0.12134343004463327	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	342	0.1202882697833756	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	342	0.1202882697833756	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	342	0.1202882697833756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	333	0.11712278899960256	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	330	0.11606762873834486	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	328	0.11536418856417309	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	327	0.1150124684770872	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	320	0.11255042786748595	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	319	0.11219870778040004	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	309	0.1086815069095411	No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT	305	0.10727462656119754	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	299	0.10516430603868217	No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	293	0.1030539855161668	TruSeq Adapter, Index 3 (95% over 23bp)
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	292	0.10270226542908092	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	289	0.10164710516782323	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	288	0.10129538508073734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.5172008708589354E-4	0.0
11	0.0	0.0	0.0	0.0010551602612576807	0.0
12	0.0	0.0	0.0	0.0010551602612576807	0.0
13	0.0	0.0	0.0	0.0010551602612576807	0.0
14	0.0	0.0	0.0	0.0010551602612576807	0.0
15	0.0	0.0	0.0	0.0010551602612576807	0.0
16	0.0	0.0	0.0	0.0014068803483435741	0.0
17	0.0	0.0	0.0	0.0014068803483435741	0.0
18	0.0	0.0	0.0	0.002462040609601255	0.0
19	0.0	0.0	0.0	0.002462040609601255	0.0
20	0.0	0.0	0.0	0.0028137606966871483	0.0
21	0.0	0.0	0.0	0.005275801306288404	0.0
22	0.0	0.0	0.0	0.007737841915889658	0.0
23	0.0	0.0	0.0	0.00984816243840502	0.0
24	0.0	0.0	0.0	0.011255042786748593	0.0
25	0.0	0.0	0.0	0.011255042786748593	0.0
26	0.0	0.0	0.0	0.012661923135092169	0.0
27	0.0	0.0	0.0	0.01582740391886521	0.0
28	0.0	0.0	0.0	0.037282329231104715	0.0
29	0.0	0.0	0.0	0.08652314142312982	0.0
30	0.0	0.0	0.0	0.13435707326681134	0.0
31	0.0	0.0	0.0	0.20681141120650542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACA	20	0.0018396073	37.0	3
AGCAACG	40	3.816058E-8	37.0	2
AATCCAG	20	0.0018396073	37.0	26
GTCGAAT	20	0.0018396073	37.0	22
TTACTTT	25	1.2312896E-4	37.0	3
TCGAATC	20	0.0018396073	37.0	23
CTTATAC	1560	0.0	34.39102	1
CGTCTTC	540	0.0	34.25926	37
TTACGGG	80	0.0	32.375	3
TTATACA	1730	0.0	30.797688	2
ATTACGG	85	0.0	30.470587	2
GATTACG	85	0.0	30.470587	1
AGCCCCC	25	0.0054894737	29.6	37
CCAGAAT	25	0.0054894737	29.6	29
TCCAGAA	25	0.0054894737	29.6	28
TCAGCCC	25	0.0054894737	29.6	35
AATACAC	25	0.0054894737	29.6	4
TATACAC	1830	0.0	29.31694	3
GACCGTT	40	5.924656E-5	27.75	7
GCAACGC	55	6.233913E-7	26.90909	3
>>END_MODULE