FastQCFastQC Report
Fri 10 Feb 2017
ERR1630901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248246
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT177747.15983339107176No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG136465.496966718496974No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT108964.389194589238095RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC76833.094913916034901No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC41211.6600468889730349RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC39091.5746477284628957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36441.4678987778252217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35661.4364783319771517No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG18410.7416030872602177No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16720.6735254545893992No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA14110.5683878088670109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12950.5216599663237274No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11980.4825858221280504No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT11410.45962472708523No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9000.36254360593926993No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT8380.33756837975234244No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7570.30493945521780813No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7460.30050836670077263No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7450.3001055404719512No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7380.2972857568702013No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6650.2678794421662383No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6530.2630455274203814No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6380.25700313398806024No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5570.22437420945352593No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5240.21108094390241938No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5200.20946963898713372No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4630.1865085439443133No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4600.18530006525784906No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4540.1828831078849206No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4510.18167462919845637No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4350.1752294095373138No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4150.16717288496088556No Hit
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT4130.16636723250324276No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4110.16556158004559993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.1647559275879571No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4070.1639502751303143No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3820.153879619409779No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3810.15347679318095758No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3800.15307396695213618No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3800.15307396695213618No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3770.15186548826567195No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3760.15146266203685055No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3580.14421178991806516No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT3370.1357524391128155TruSeq Adapter, Index 3 (95% over 23bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3330.13414113419752985No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3220.12971004568049435No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3200.12890439322285152No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3140.12648743584992306No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3110.12527895716345883No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3000.12084786864642331No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2960.11923656373113765No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.11883373750231625No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2940.11843091127349485No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2810.11319417029881648No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2810.11319417029881648No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2780.11198569161235225No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2760.11118003915470945No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2690.10836025555295956No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2620.10554047195120969No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2570.10352634080710262No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2530.10191503589181698No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2490.10030373097653135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA308.285731E-637.01
TTATAGT200.001839156837.012
GCCGAGC308.285731E-637.03
ATAGTTA200.001839156837.014
TTGTTAG200.001839156837.03
GATACAC251.2308388E-436.9999963
TTTACAC251.2308388E-436.9999962
CTTATAC19300.036.1373061
TTATACA20600.034.0364072
TATACAC21800.032.417433
TTACGGG401.5933838E-632.3753
GATTACG401.5933838E-632.3751
CGCCGAG352.3808243E-531.7142872
AGCAACG303.5914223E-430.8333342
TCAGAGT303.5914223E-430.8333343
CGTCTTC5150.030.5339837
TTAGGAC551.8433639E-830.2727283
TAGGACC551.8433639E-830.2727284
CCGGGAC250.005488147529.5999989
ATTACGG453.991632E-628.7777772
Produced by FastQC (version 0.11.5)