##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248246
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240257647655955	31.0	31.0	34.0	28.0	34.0
2	31.271609613045126	31.0	31.0	34.0	28.0	34.0
3	30.73956881480467	31.0	31.0	34.0	26.0	34.0
4	34.87833036584678	37.0	35.0	37.0	32.0	37.0
5	35.0959733490167	35.0	35.0	37.0	32.0	37.0
6	35.29184760278111	37.0	35.0	37.0	32.0	37.0
7	35.27783327828042	37.0	35.0	37.0	32.0	37.0
8	35.30505224656188	37.0	35.0	37.0	32.0	37.0
9	37.11974009651716	39.0	37.0	39.0	33.0	39.0
10	36.941743270787846	39.0	37.0	39.0	33.0	39.0
11	36.981179958589465	39.0	37.0	39.0	33.0	39.0
12	36.87194959838225	39.0	37.0	39.0	33.0	39.0
13	36.962202814949684	39.0	37.0	39.0	33.0	39.0
14	37.81699201598415	39.0	37.0	41.0	33.0	41.0
15	37.82292161807239	39.0	37.0	41.0	33.0	41.0
16	37.80013776657026	39.0	37.0	41.0	32.0	41.0
17	37.81055082458529	39.0	37.0	41.0	33.0	41.0
18	37.97334901670118	40.0	37.0	41.0	33.0	41.0
19	37.94142100980479	40.0	37.0	41.0	33.0	41.0
20	38.04749321237804	40.0	37.0	41.0	33.0	41.0
21	37.924498279932	40.0	37.0	41.0	33.0	41.0
22	37.71924623156063	39.0	37.0	41.0	32.0	41.0
23	37.80048419712704	39.0	37.0	41.0	33.0	41.0
24	37.632372727053	39.0	37.0	41.0	32.0	41.0
25	37.57667797265616	39.0	36.0	41.0	32.0	41.0
26	37.434230561620325	39.0	36.0	41.0	32.0	41.0
27	37.290574671898035	39.0	36.0	41.0	32.0	41.0
28	37.36037640082821	39.0	36.0	41.0	32.0	41.0
29	37.20381798699677	39.0	36.0	40.0	31.0	41.0
30	37.02095502042329	39.0	35.0	40.0	31.0	41.0
31	36.96719786018707	39.0	35.0	40.0	31.0	41.0
32	36.68002706992257	38.0	35.0	40.0	31.0	41.0
33	36.531936063420964	38.0	35.0	40.0	30.0	41.0
34	36.46530457691161	38.0	35.0	40.0	30.0	41.0
35	36.41283243234533	38.0	35.0	40.0	30.0	41.0
36	36.3264745454106	38.0	35.0	40.0	30.0	41.0
37	36.23205207737486	38.0	35.0	40.0	30.0	41.0
38	36.14790973469864	38.0	35.0	40.0	30.0	41.0
39	35.91662302715854	38.0	35.0	40.0	29.0	41.0
40	35.63321866213353	38.0	35.0	40.0	27.0	41.0
41	35.5313398806023	38.0	34.0	40.0	27.0	41.0
42	35.345391265116056	38.0	34.0	40.0	26.0	41.0
43	34.362958516954954	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	9.0
11	11.0
12	8.0
13	6.0
14	9.0
15	8.0
16	10.0
17	11.0
18	13.0
19	35.0
20	42.0
21	97.0
22	204.0
23	334.0
24	573.0
25	906.0
26	1530.0
27	2376.0
28	3322.0
29	4482.0
30	5760.0
31	7392.0
32	9532.0
33	11684.0
34	15131.0
35	20162.0
36	26587.0
37	37734.0
38	54097.0
39	46169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3664993595063	22.740749095655115	17.00853185952644	26.88421968531215
2	11.976829435318193	20.664582712309564	43.727592791021806	23.630995061350436
3	13.545434770348766	30.891535009627546	37.092641976104346	18.470388243919338
4	9.320190456240987	21.60316782546345	28.556754187378647	40.51988753091692
5	13.132135059577998	36.9750167172885	31.4438097693417	18.449038453791804
6	25.340589576468503	38.244724990533584	15.19863361343184	21.216051819566076
7	21.356235347195927	33.391877411922046	15.546272648904717	29.705614591977316
8	27.036487999806642	29.52434278900768	23.86100883800746	19.578160373178218
9	20.60979834518985	11.288802236491223	14.373242670576767	53.72815674774216
10	15.853629061495452	27.660063002022188	26.79841769857319	29.68789023790917
11	30.02344448651741	19.554796451906576	23.72082531037761	26.70093375119841
12	19.32437984902073	26.265478597842463	21.224511170371326	33.18563038276549
13	35.24729502187346	16.254038332943935	25.015105983580803	23.4835606616018
14	23.0847626950686	24.028181722968345	22.10428365411729	30.782771927845765
15	28.36863433851905	24.067658693392843	18.35437429001877	29.209332678069334
16	30.013776657025694	21.433175156900816	17.872594120348364	30.680454065725126
17	18.529603699556084	34.310321213634865	18.60251524697276	28.557559839836294
18	28.215963197795734	16.281027690274968	21.159656147531077	34.343352964398214
19	24.561926476156714	23.808641428260678	21.319175334144358	30.310256761438254
20	20.50586917815393	18.87643708257132	23.38124279948116	37.23645093979359
21	28.088670109488167	19.23334112130709	26.377061463225992	26.30092730597875
22	18.001498513571214	40.32854507222674	21.402157537281568	20.267798876920473
23	26.165174866865932	21.87064444140087	23.634620497409827	28.32956019432337
24	29.82968507045431	26.09951419156804	22.140135188482393	21.93066554949526
25	18.289922093407345	32.85893831119132	22.377399837258203	26.473739758143132
26	27.310812661634028	20.92521128235702	24.30613182085512	27.45784423515384
27	18.1912296673461	35.06682887136147	23.802599034828358	22.939342426464073
28	16.705606535452734	22.044262546022896	32.434359466013554	28.815771452510813
29	20.971536298671477	25.80021430355373	22.338728519291347	30.889520878483438
30	16.610942371679705	27.58634580214787	29.186371583026517	26.616340243145913
31	27.44132835977216	17.741272769752584	28.744471210009426	26.07292766046583
32	20.642427269724386	34.69824287198988	26.135365725933145	18.523964132352585
33	17.875011077721293	27.106579763621568	30.271585443471395	24.74682371518574
34	24.863240495315132	28.352924115595012	24.316202476575654	22.467632912514198
35	18.585999371591083	26.85843880666758	36.381250856005735	18.1743109657356
36	22.528056846837412	19.889142221828347	22.56471403366016	35.01808689767408
37	21.683330244998913	25.52588964172635	29.15656244209373	23.634217671181005
38	20.44302828645779	17.850035851534365	31.947342555368465	29.75959330663938
39	27.953723322832996	19.451270111099475	25.32447652731565	27.270530038751883
40	18.461123240656445	18.238360336118205	41.73279730589818	21.567719117327165
41	23.982259532882704	21.340525124271892	23.742980752962787	30.934234589882614
42	25.172611039049976	17.56926597004584	35.2066095727625	22.05151341814168
43	24.276725506151156	17.887901517043577	33.514739411712576	24.320633565092688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	6.0
2	10.0
3	54.0
4	98.0
5	98.0
6	143.5
7	189.0
8	222.5
9	256.0
10	390.5
11	525.0
12	525.0
13	847.0
14	1169.0
15	3243.5
16	5318.0
17	5373.5
18	5429.0
19	5429.0
20	4308.5
21	3188.0
22	2478.5
23	1769.0
24	1701.5
25	1634.0
26	1634.0
27	1603.5
28	1573.0
29	1591.5
30	1610.0
31	1778.0
32	1946.0
33	1946.0
34	2209.5
35	2473.0
36	2569.5
37	2666.0
38	2958.0
39	3250.0
40	3250.0
41	4094.5
42	4939.0
43	6173.5
44	7408.0
45	10319.5
46	13231.0
47	13231.0
48	18639.5
49	24048.0
50	38344.5
51	52641.0
52	38367.5
53	24094.0
54	24094.0
55	32326.0
56	40558.0
57	33161.0
58	25764.0
59	17458.5
60	9153.0
61	9153.0
62	7568.0
63	5983.0
64	4716.0
65	3449.0
66	2844.0
67	2239.0
68	2239.0
69	1626.5
70	1014.0
71	715.0
72	416.0
73	257.5
74	99.0
75	99.0
76	82.0
77	65.0
78	41.0
79	17.0
80	14.0
81	11.0
82	11.0
83	9.5
84	8.0
85	4.5
86	1.0
87	2.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	248246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.49074708152397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83393613723261	13.806063340396218
2	11.402294099410975	4.444784608815449
3	4.896145499638318	2.862886008233768
4	2.6743825565774517	2.0850285603796235
5	1.8497468223623021	1.8026473739758144
6	1.1635837552960628	1.3607470009587266
7	0.865970858737212	1.1814893291331985
8	0.6675622610313113	1.040902975274526
9	0.5497571561434328	0.9643659917984579
>10	4.15211325824119	16.40106990646375
>50	0.504288519169164	6.949960925855804
>100	0.3864834142812855	14.036882769510889
>500	0.024801074713237573	3.3358040008701044
>1k	0.02066756226103131	9.586458593491939
>5k	0.002066756226103131	3.094913916034901
>10k+	0.006200268678309393	17.04599469880683
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	17774	7.15983339107176	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	13646	5.496966718496974	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	10896	4.389194589238095	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	7683	3.094913916034901	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	4121	1.6600468889730349	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	3909	1.5746477284628957	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3644	1.4678987778252217	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3566	1.4364783319771517	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1841	0.7416030872602177	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1672	0.6735254545893992	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA	1411	0.5683878088670109	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1295	0.5216599663237274	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1198	0.4825858221280504	No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT	1141	0.45962472708523	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	900	0.36254360593926993	No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT	838	0.33756837975234244	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	757	0.30493945521780813	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	746	0.30050836670077263	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	745	0.3001055404719512	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	738	0.2972857568702013	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	665	0.2678794421662383	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	653	0.2630455274203814	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	638	0.25700313398806024	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	557	0.22437420945352593	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	524	0.21108094390241938	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	520	0.20946963898713372	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	463	0.1865085439443133	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	460	0.18530006525784906	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	454	0.1828831078849206	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	451	0.18167462919845637	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	435	0.1752294095373138	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	415	0.16717288496088556	No Hit
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT	413	0.16636723250324276	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	411	0.16556158004559993	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	409	0.1647559275879571	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	407	0.1639502751303143	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	382	0.153879619409779	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	381	0.15347679318095758	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	380	0.15307396695213618	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	380	0.15307396695213618	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	377	0.15186548826567195	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	376	0.15146266203685055	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	358	0.14421178991806516	No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT	337	0.1357524391128155	TruSeq Adapter, Index 3 (95% over 23bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	333	0.13414113419752985	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	322	0.12971004568049435	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	320	0.12890439322285152	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	314	0.12648743584992306	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	311	0.12527895716345883	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	300	0.12084786864642331	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	296	0.11923656373113765	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	295	0.11883373750231625	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	294	0.11843091127349485	No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC	281	0.11319417029881648	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	281	0.11319417029881648	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	278	0.11198569161235225	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	276	0.11118003915470945	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	269	0.10836025555295956	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	262	0.10554047195120969	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	257	0.10352634080710262	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	253	0.10191503589181698	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	249	0.10030373097653135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.0282622882141105E-4	0.0
11	0.0	0.0	0.0	4.0282622882141105E-4	0.0
12	0.0	0.0	0.0	8.056524576428221E-4	0.0
13	0.0	0.0	0.0	8.056524576428221E-4	0.0
14	0.0	0.0	0.0	8.056524576428221E-4	0.0
15	0.0	0.0	0.0	8.056524576428221E-4	0.0
16	0.0	0.0	0.0	8.056524576428221E-4	0.0
17	0.0	0.0	0.0	8.056524576428221E-4	0.0
18	0.0	0.0	0.0	8.056524576428221E-4	0.0
19	0.0	0.0	0.0	8.056524576428221E-4	0.0
20	0.0	0.0	0.0	0.0012084786864642331	0.0
21	0.0	0.0	0.0	0.0016113049152856442	0.0
22	0.0	0.0	0.0	0.0024169573729284663	0.0
23	0.0	0.0	0.0	0.002819783601749877	0.0
24	0.0	0.0	0.0	0.0032226098305712884	0.0
25	0.0	0.0	0.0	0.0032226098305712884	0.0
26	0.0	0.0	0.0	0.00402826228821411	0.0
27	0.0	0.0	0.0	0.006445219661142577	0.0
28	0.0	0.0	0.0	0.017724354068142083	0.0
29	0.0	0.0	0.0	0.041088275339783924	0.0
30	0.0	0.0	0.0	0.07774546216253232	0.0
31	0.0	0.0	0.0	0.13736374402810117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	30	8.285731E-6	37.0	1
TTATAGT	20	0.0018391568	37.0	12
GCCGAGC	30	8.285731E-6	37.0	3
ATAGTTA	20	0.0018391568	37.0	14
TTGTTAG	20	0.0018391568	37.0	3
GATACAC	25	1.2308388E-4	36.999996	3
TTTACAC	25	1.2308388E-4	36.999996	2
CTTATAC	1930	0.0	36.137306	1
TTATACA	2060	0.0	34.036407	2
TATACAC	2180	0.0	32.41743	3
TTACGGG	40	1.5933838E-6	32.375	3
GATTACG	40	1.5933838E-6	32.375	1
CGCCGAG	35	2.3808243E-5	31.714287	2
AGCAACG	30	3.5914223E-4	30.833334	2
TCAGAGT	30	3.5914223E-4	30.833334	3
CGTCTTC	515	0.0	30.53398	37
TTAGGAC	55	1.8433639E-8	30.272728	3
TAGGACC	55	1.8433639E-8	30.272728	4
CCGGGAC	25	0.0054881475	29.599998	9
ATTACGG	45	3.991632E-6	28.777777	2
>>END_MODULE