FastQCFastQC Report
Fri 10 Feb 2017
ERR1630900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831475
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT161311.9400463032562616No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG123111.4806217865840825No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT120641.450915541657897RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC64520.7759704140232718No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC47290.5687483087284645RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC34490.41480501518386004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28260.33987792777894704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.33169969030938995No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG14730.17715505577437687No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA13090.1574310712889744No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12730.1531014161580324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10930.1314531405033224No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT10310.12399651222225563No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8380.10078474999248323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19850.032.9924431
CGTCTTC6000.032.06666637
TTATACA21750.030.620692
TATACAC22550.029.4523263
GGTATCA5050.026.0099011
CCGTCTT18050.024.49584437
GCCGTCT18350.023.9945536
CCGTCCG400.001930740223.1259
CCGTTTA400.001930740223.12527
GTATCAA13600.022.1727941
GATTACG705.099124E-621.1428571
ACGCAAT450.003824931820.55555511
TGCCGTC21700.020.37557635
CGGATCG555.14145E-420.18181837
ATGCCGT22600.019.64601734
TACACAT35750.019.0951045
ATACACA36050.018.7822484
TATGCCG23950.018.53862233
CGCGATA2100.018.50000234
AAGCTAG801.615794E-518.55
Produced by FastQC (version 0.11.5)