##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630900.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831475
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4181941730058	31.0	31.0	34.0	28.0	34.0
2	31.420136504404823	31.0	31.0	34.0	28.0	34.0
3	30.89561682552091	31.0	31.0	34.0	27.0	34.0
4	35.05049340028263	37.0	35.0	37.0	32.0	37.0
5	35.2399122042154	37.0	35.0	37.0	33.0	37.0
6	35.43107970774828	37.0	35.0	37.0	33.0	37.0
7	35.435374485101775	37.0	35.0	37.0	33.0	37.0
8	35.46420397486395	37.0	35.0	37.0	33.0	37.0
9	37.22926365795725	39.0	37.0	39.0	34.0	39.0
10	37.06132234883791	39.0	37.0	39.0	33.0	39.0
11	37.11237499624162	39.0	37.0	39.0	33.0	39.0
12	37.05054992633573	39.0	37.0	39.0	33.0	39.0
13	37.134565681469674	39.0	37.0	39.0	33.0	39.0
14	38.24326888962386	40.0	38.0	41.0	33.0	41.0
15	38.25145073513936	40.0	38.0	41.0	33.0	41.0
16	38.246862503382545	40.0	38.0	41.0	33.0	41.0
17	38.206774707597944	40.0	38.0	41.0	33.0	41.0
18	38.267086803571964	40.0	38.0	41.0	33.0	41.0
19	38.26661655491747	40.0	38.0	41.0	34.0	41.0
20	38.26984695871794	40.0	38.0	41.0	34.0	41.0
21	38.23196488168616	40.0	38.0	41.0	34.0	41.0
22	38.152753841065575	40.0	37.0	41.0	33.0	41.0
23	38.1683321807631	40.0	38.0	41.0	33.0	41.0
24	38.09734868757329	40.0	37.0	41.0	33.0	41.0
25	38.066247331549356	40.0	37.0	41.0	33.0	41.0
26	37.948501157581404	40.0	37.0	41.0	33.0	41.0
27	37.87383505216633	40.0	37.0	41.0	33.0	41.0
28	37.88724375357046	40.0	37.0	41.0	33.0	41.0
29	37.79574010042395	40.0	37.0	41.0	33.0	41.0
30	37.70954749090472	40.0	37.0	41.0	33.0	41.0
31	37.696242220150936	40.0	37.0	41.0	33.0	41.0
32	37.582227968369466	40.0	37.0	41.0	32.0	41.0
33	37.55154514567486	39.0	37.0	41.0	32.0	41.0
34	37.47804203373523	39.0	37.0	41.0	32.0	41.0
35	37.45478577227217	39.0	36.0	41.0	32.0	41.0
36	37.43154755103882	39.0	36.0	41.0	32.0	41.0
37	37.3712727382062	39.0	36.0	41.0	32.0	41.0
38	37.34938753420127	39.0	36.0	41.0	32.0	41.0
39	37.25020595928922	39.0	36.0	41.0	31.0	41.0
40	37.14649989476533	39.0	36.0	41.0	31.0	41.0
41	37.094697976487566	39.0	36.0	41.0	31.0	41.0
42	37.02861060164166	39.0	36.0	41.0	31.0	41.0
43	36.35895968008659	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	12.0
11	20.0
12	7.0
13	11.0
14	9.0
15	12.0
16	9.0
17	10.0
18	22.0
19	37.0
20	87.0
21	146.0
22	303.0
23	616.0
24	1163.0
25	1985.0
26	3140.0
27	4843.0
28	7239.0
29	10483.0
30	14122.0
31	18976.0
32	24542.0
33	31962.0
34	42847.0
35	57094.0
36	79316.0
37	116782.0
38	201824.0
39	213845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.72127845094561	22.279563426440962	15.234492919209838	24.76466520340359
2	13.904687453020234	21.77010733936679	41.01650681018671	23.308698397426262
3	14.532848251601072	28.710544514266818	34.22063200938092	22.535975224751194
4	11.551880693947503	19.512312456778616	37.86175170630506	31.07405514296882
5	12.527135512192189	38.162422201509365	35.70558345109595	13.604858835202501
6	27.57388977419646	40.31137436483358	17.58729967828257	14.527436182687392
7	24.55371478396825	33.020595928921495	22.974954147749482	19.450735139360773
8	24.987401906250938	34.95547069966024	22.21630235424998	17.84082503983884
9	23.770528278060073	14.880303075859166	19.776421419766077	41.57274722631468
10	14.693406296040171	28.573559036651737	33.35061186445774	23.382422802850357
11	31.132625755434617	23.20995820680117	25.503232207823444	20.154183829940767
12	20.401936317989115	26.611503653146517	29.049700832857273	23.936859196007095
13	29.043927959349347	20.510297964460747	26.773985988754923	23.67178808743498
14	21.79211641961574	22.230012928831293	26.49917315613819	29.478697495414774
15	24.072401455245195	28.794130911933614	22.68510779037253	24.44835984244866
16	24.84572596891067	26.947292462190685	23.876244024173907	24.330737544724737
17	20.773324513665475	30.723713881956765	25.09071228840314	23.412249315974623
18	23.698487627409122	26.04059051685258	25.810156649328004	24.450765206410292
19	23.318319853272797	28.941699990979885	25.756637301181634	21.98334285456568
20	22.681379476231996	27.20526774707598	25.665955079828016	24.447397696864005
21	25.168525812562017	26.485462581556874	25.62506389248023	22.720947713400886
22	21.722601401124507	31.325415676959622	25.952313659460597	20.999669262455274
23	25.269310562554498	25.273038876695033	26.02230975074416	23.435340810006313
24	24.320995820680118	28.427673712378603	25.568417571183737	21.68291289575754
25	22.057788869178268	28.68240175591569	25.9496677591028	23.31014161580324
26	24.423103520851498	29.27135512192189	25.69289515619832	20.61264620102829
27	21.159746234102048	31.239063110736943	26.16410595628251	21.4370846988785
28	23.29270272708139	25.914789981659098	27.658919390240232	23.133587901019272
29	22.334405724766228	29.354340178598274	26.142096876033555	22.169157220601942
30	22.83315794221113	27.731441113683513	27.50100724615893	21.93439369794642
31	26.081722240596527	25.755314351002735	25.680748068192067	22.48221534020867
32	22.321416759373403	27.848101265822784	27.091373763492587	22.739108211311223
33	23.12973931868066	27.2498872485643	28.263387353798976	21.356986078956073
34	23.499564027781954	27.745753029255237	26.555097868246193	22.19958507471662
35	21.491445924411437	25.71370155446646	30.039508103069846	22.755344418052257
36	23.164857632520523	27.780270002104697	25.345320063742143	23.70955230163264
37	22.124056646321296	26.657566373011814	27.97642743317598	23.241949547490908
38	22.215340208665324	25.153973360594122	28.391954057548336	24.238732373192217
39	24.01539432935446	25.514176613848882	26.959920622989266	23.510508433807388
40	21.24790282329595	25.01049340028263	31.681890616073844	22.059713160347574
41	22.460687332752034	25.780690940797978	26.452148290688232	25.30647343576175
42	22.713250548723654	25.228659911602875	29.778526113232505	22.279563426440962
43	23.0643134189242	24.59689106707959	29.60906822213536	22.72972729186085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	89.0
4	166.0
5	166.0
6	214.5
7	263.0
8	293.5
9	324.0
10	447.5
11	571.0
12	571.0
13	905.0
14	1239.0
15	2896.0
16	4553.0
17	5077.0
18	5601.0
19	5601.0
20	5701.5
21	5802.0
22	6738.5
23	7675.0
24	9913.0
25	12151.0
26	12151.0
27	15160.5
28	18170.0
29	21664.5
30	25159.0
31	29231.0
32	33303.0
33	33303.0
34	37893.5
35	42484.0
36	45977.0
37	49470.0
38	52526.5
39	55583.0
40	55583.0
41	57752.0
42	59921.0
43	61122.5
44	62324.0
45	62822.5
46	63321.0
47	63321.0
48	63578.5
49	63836.0
50	74529.0
51	85222.0
52	68021.0
53	50820.0
54	50820.0
55	57730.5
56	64641.0
57	53979.0
58	43317.0
59	34069.0
60	24821.0
61	24821.0
62	21699.0
63	18577.0
64	15231.0
65	11885.0
66	9845.0
67	7805.0
68	7805.0
69	6329.0
70	4853.0
71	3949.0
72	3045.0
73	2574.0
74	2103.0
75	2103.0
76	1738.0
77	1373.0
78	996.0
79	619.0
80	450.5
81	282.0
82	282.0
83	193.0
84	104.0
85	75.5
86	47.0
87	33.5
88	20.0
89	20.0
90	13.5
91	7.0
92	4.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831475.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.238777841087945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23134886707194	40.59717482649635
2	12.243324062340854	12.546659233334587
3	3.477052271209037	5.344797265989903
4	1.5203519122569782	3.1160389548963408
5	0.7771555702439261	1.9910250805846272
6	0.48665834152110043	1.496146718742718
7	0.34526940481295904	1.2383827629975106
8	0.23709621186462732	0.9718816101356127
9	0.18394323473659976	0.8482523886035752
>10	1.2861705291945582	12.990212682695748
>50	0.14508422714835276	5.062058022296611
>100	0.06160737212441694	5.1445555295994305
>500	0.0018811411332114868	0.5925413731344572
>1k	0.0021162837748629226	2.4025757465787336
>5k	2.3514264165143585E-4	0.7773641601186493
>10k+	7.054279249543075E-4	4.880333643795104
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	16131	1.9400463032562616	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	12311	1.4806217865840825	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	12064	1.450915541657897	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6452	0.7759704140232718	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	4729	0.5687483087284645	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	3449	0.41480501518386004	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2826	0.33987792777894704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2758	0.33169969030938995	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1473	0.17715505577437687	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1309	0.1574310712889744	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1273	0.1531014161580324	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.1314531405033224	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1031	0.12399651222225563	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	838	0.10078474999248323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.2026819808172224E-4	0.0	0.0	0.0	0.0
3	1.2026819808172224E-4	0.0	0.0	0.0	0.0
4	1.2026819808172224E-4	0.0	0.0	0.0	0.0
5	2.4053639616344449E-4	0.0	0.0	0.0	0.0
6	2.4053639616344449E-4	0.0	0.0	0.0	0.0
7	2.4053639616344449E-4	0.0	0.0	0.0	0.0
8	3.6080459424516674E-4	0.0	0.0	0.0	0.0
9	3.6080459424516674E-4	0.0	0.0	1.2026819808172224E-4	0.0
10	7.216091884903335E-4	0.0	0.0	1.2026819808172224E-4	0.0
11	8.418773865720557E-4	0.0	0.0	2.4053639616344449E-4	0.0
12	8.418773865720557E-4	0.0	0.0	2.4053639616344449E-4	0.0
13	8.418773865720557E-4	0.0	0.0	2.4053639616344449E-4	0.0
14	8.418773865720557E-4	0.0	0.0	2.4053639616344449E-4	0.0
15	0.001443218376980667	0.0	0.0	3.6080459424516674E-4	0.0
16	0.001443218376980667	0.0	0.0	3.6080459424516674E-4	0.0
17	0.0016837547731441114	0.0	0.0	6.013409904086113E-4	0.0
18	0.0016837547731441114	0.0	0.0	7.216091884903335E-4	0.0
19	0.0018040229712258337	0.0	0.0	7.216091884903335E-4	0.0
20	0.0022850957635527224	0.0	0.0	7.216091884903335E-4	0.0
21	0.0022850957635527224	0.0	0.0	0.0019242911693075559	0.0
22	0.002405363961634445	0.0	0.0	0.0022850957635527224	0.0
23	0.002405363961634445	0.0	0.0	0.0027661685558796117	0.0
24	0.002405363961634445	0.0	0.0	0.0027661685558796117	0.0
25	0.002405363961634445	0.0	0.0	0.003006704952043056	0.0
26	0.002405363961634445	0.0	0.0	0.0032472413482065006	0.0
27	0.0025256321597161673	0.0	0.0	0.004089118734778556	0.0
28	0.0027661685558796117	0.0	0.0	0.00962145584653778	0.0
29	0.002886436753961334	0.0	0.0	0.025015785200998227	0.0
30	0.002886436753961334	0.0	0.0	0.03980877356505006	0.0
31	0.002886436753961334	0.0	0.0	0.08094049730899906	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1985	0.0	32.992443	1
CGTCTTC	600	0.0	32.066666	37
TTATACA	2175	0.0	30.62069	2
TATACAC	2255	0.0	29.452326	3
GGTATCA	505	0.0	26.009901	1
CCGTCTT	1805	0.0	24.495844	37
GCCGTCT	1835	0.0	23.99455	36
CCGTCCG	40	0.0019307402	23.125	9
CCGTTTA	40	0.0019307402	23.125	27
GTATCAA	1360	0.0	22.172794	1
GATTACG	70	5.099124E-6	21.142857	1
ACGCAAT	45	0.0038249318	20.555555	11
TGCCGTC	2170	0.0	20.375576	35
CGGATCG	55	5.14145E-4	20.181818	37
ATGCCGT	2260	0.0	19.646017	34
TACACAT	3575	0.0	19.095104	5
ATACACA	3605	0.0	18.782248	4
TATGCCG	2395	0.0	18.538622	33
CGCGATA	210	0.0	18.500002	34
AAGCTAG	80	1.615794E-5	18.5	5
>>END_MODULE