FastQCFastQC Report
Fri 10 Feb 2017
ERR1630899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794359
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT142381.7923885799745454No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG129661.6322594695849106No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT99131.2479244271167067TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC72760.9159586534551758No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC33580.42273078041540413Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC32680.41140089052934503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20300.2555519607633324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.2481245885046937No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG17140.21577145849672502No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA11770.14816978217657256No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT9290.11694964115720977No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9050.11392833718759403No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT8680.10927049356776973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17350.033.0547561
TTATACA19300.030.2901552
GATTACG551.8542778E-830.2727281
TATACAC19450.029.961443
CGTCTTC4450.028.68539437
CCGTCTT16050.026.85669937
GACCGTT358.866975E-426.428577
GCCGTCT16450.026.09118536
GGTATCA4250.025.6823541
GTATCAA11750.024.2468091
ATTACGG753.7377504E-722.22
TTACGGG753.7377504E-722.23
TGCCGTC20150.021.30024735
ATGCCGT20900.020.62440134
CCGTTAA450.003824783720.55555516
TCGCACC555.1411707E-420.18181828
TATGCCG22700.018.98898733
TCGCCGA609.2333916E-418.51
AAGTACG609.2333916E-418.535
ACGCATC500.00703301818.4999981
Produced by FastQC (version 0.11.5)