##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794359
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210306674941684	31.0	31.0	34.0	28.0	34.0
2	31.21128356322519	31.0	31.0	34.0	28.0	34.0
3	30.658544814120567	31.0	31.0	34.0	26.0	34.0
4	34.85844939127019	37.0	35.0	37.0	32.0	37.0
5	35.03549276838306	35.0	35.0	37.0	32.0	37.0
6	35.23164337535044	37.0	35.0	37.0	32.0	37.0
7	35.244089888828604	37.0	35.0	37.0	32.0	37.0
8	35.268107996510395	37.0	35.0	37.0	32.0	37.0
9	37.01015409909122	39.0	37.0	39.0	33.0	39.0
10	36.81622666829482	39.0	37.0	39.0	33.0	39.0
11	36.868913174018296	39.0	37.0	39.0	33.0	39.0
12	36.79300039402839	39.0	37.0	39.0	33.0	39.0
13	36.87023751225831	39.0	37.0	39.0	33.0	39.0
14	37.92868211979722	40.0	37.0	41.0	33.0	41.0
15	37.94373450794917	40.0	37.0	41.0	33.0	41.0
16	37.93583128031533	40.0	37.0	41.0	33.0	41.0
17	37.905940513042594	40.0	37.0	41.0	33.0	41.0
18	37.95056517267382	40.0	37.0	41.0	33.0	41.0
19	37.96772366146793	40.0	37.0	41.0	33.0	41.0
20	37.98299761191099	40.0	37.0	41.0	33.0	41.0
21	37.92313928588963	40.0	37.0	41.0	33.0	41.0
22	37.843802361400826	40.0	37.0	41.0	33.0	41.0
23	37.84586943686671	39.0	37.0	41.0	33.0	41.0
24	37.79328615902885	39.0	37.0	41.0	33.0	41.0
25	37.74080988570659	39.0	37.0	41.0	32.0	41.0
26	37.63953451776842	39.0	37.0	41.0	32.0	41.0
27	37.55958955585573	39.0	37.0	41.0	32.0	41.0
28	37.549577709826416	39.0	37.0	41.0	32.0	41.0
29	37.479780552621676	39.0	37.0	40.0	32.0	41.0
30	37.39635857339062	39.0	36.0	40.0	32.0	41.0
31	37.398497404825775	39.0	36.0	40.0	32.0	41.0
32	37.250915518046625	39.0	36.0	40.0	31.0	41.0
33	37.25397836494582	39.0	36.0	40.0	31.0	41.0
34	37.155325237077946	39.0	36.0	40.0	31.0	41.0
35	37.12548482487137	39.0	36.0	40.0	31.0	41.0
36	37.1350913126181	39.0	36.0	40.0	31.0	41.0
37	37.03696313631494	39.0	36.0	40.0	31.0	41.0
38	37.001340703636515	39.0	36.0	40.0	31.0	41.0
39	36.93846107364554	39.0	36.0	40.0	31.0	41.0
40	36.83887134154708	39.0	36.0	40.0	30.0	41.0
41	36.80664661695782	39.0	35.0	40.0	30.0	41.0
42	36.76028093091411	39.0	35.0	40.0	30.0	41.0
43	36.010311458672966	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	10.0
11	15.0
12	14.0
13	11.0
14	4.0
15	10.0
16	13.0
17	9.0
18	31.0
19	55.0
20	92.0
21	194.0
22	394.0
23	701.0
24	1308.0
25	2225.0
26	3533.0
27	5467.0
28	7983.0
29	11372.0
30	15274.0
31	20346.0
32	26440.0
33	34126.0
34	44770.0
35	59943.0
36	83444.0
37	122847.0
38	202189.0
39	151531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.59521828291742	22.799766856043675	15.281881365981882	24.32313349505702
2	13.896739383578458	22.340025102000478	40.820082607486036	22.943152906935023
3	15.084363618968249	28.202236016712845	34.920986606811276	21.792413757507624
4	11.983624532484683	19.431516480583717	37.221583692008274	31.363275294923326
5	12.430903407653213	38.54050876241095	35.49100595574545	13.537581874190385
6	27.455092722559947	39.937987736023636	17.78276572683132	14.824153814585092
7	23.88743628510535	34.05739722216278	22.268017357391308	19.78714913534057
8	24.512090880823408	35.365621841006394	22.37288178267005	17.749405495500145
9	23.695457595369348	15.125025334892662	19.409612026804	41.769905042933985
10	14.328156412906507	28.821477442818676	33.65367547922287	23.196690665051946
11	31.069705259208995	22.757468600469057	25.646212858417922	20.526613281904027
12	20.110302772424056	26.363269000540058	29.308662707919215	24.21776551911667
13	28.60180346669453	20.841080670074867	27.173607902724083	23.38350796050652
14	21.280050959326953	22.585506049531762	26.585334842306818	29.54910814883447
15	23.883281992147126	28.631890618725286	22.98633237616745	24.498495012960138
16	24.463498241978755	27.980296062611487	23.32572552208762	24.23048017332214
17	20.90100319880558	30.758510950338575	25.056806809012045	23.2836790418438
18	23.336551861312078	26.54945685766763	25.761651847590322	24.35233943342997
19	23.682994716494683	28.831296680719927	25.628462697596426	21.857245905188964
20	22.445518965606233	25.77814313175781	26.28383388367224	25.492504018963718
21	24.235138016941963	26.231212839534766	26.62599655823123	22.90765258529204
22	21.930512526452144	30.907310170842152	25.613104402417548	21.54907290028816
23	22.97223295764258	27.15862727054141	26.242290954090024	23.62684881772599
24	25.040693187840763	28.065396124422332	25.457255472651536	21.43665521508537
25	22.306161320007703	27.47498297369326	25.682090843057104	24.536764863241935
26	24.987190930045482	27.751054623916893	26.178088244735694	21.08366620130193
27	22.14792052459908	29.044676273574037	25.68322383204571	23.12417936978117
28	21.052571947947968	26.879030765686547	28.654424510832005	23.41397277553348
29	23.981977921821244	27.98457624323511	25.620154111679984	22.41329172326366
30	21.086813392936946	27.721848685543943	27.28816567823868	23.903172243280434
31	25.043085053483377	25.412565351434303	27.25530899756911	22.289040597513214
32	23.79516062636667	26.557010117591666	26.860902941868854	22.786926314172813
33	22.831616435390046	27.450938429601727	28.32963433409831	21.387810800909914
34	23.229043795059916	28.268201153382787	26.09487649790586	22.407878553651432
35	21.612142620653884	25.80243945118013	29.81397579683745	22.77144213132853
36	24.69727163662777	25.936761590162632	25.44353371712286	23.92243305608673
37	22.68130656290166	26.555499465606857	27.861583994138673	22.90160997735281
38	22.352362093209745	25.207494344496634	28.330641435421516	24.10950212687211
39	24.117558937457748	25.847129572397364	26.62851431153924	23.406797178605643
40	21.27161648574511	25.031377500601117	31.423827261981046	22.27317875167273
41	22.475732005302387	26.357981718593233	26.527678291553315	24.63860798455107
42	23.040715847620536	25.05303017905003	29.42661945039963	22.47963452292981
43	22.906519596303436	24.810948198484564	29.191335403765805	23.0911968014462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	11.0
2	16.0
3	64.5
4	113.0
5	113.0
6	148.5
7	184.0
8	200.5
9	217.0
10	326.5
11	436.0
12	436.0
13	709.5
14	983.0
15	2313.0
16	3643.0
17	4202.5
18	4762.0
19	4762.0
20	5297.0
21	5832.0
22	7404.5
23	8977.0
24	11529.5
25	14082.0
26	14082.0
27	17001.0
28	19920.0
29	24228.0
30	28536.0
31	31408.0
32	34280.0
33	34280.0
34	37918.5
35	41557.0
36	44137.5
37	46718.0
38	49720.0
39	52722.0
40	52722.0
41	54547.5
42	56373.0
43	54169.5
44	51966.0
45	52336.5
46	52707.0
47	52707.0
48	52730.0
49	52753.0
50	54131.5
51	55510.0
52	68502.0
53	81494.0
54	81494.0
55	62269.0
56	43044.0
57	47534.5
58	52025.0
59	42755.5
60	33486.0
61	33486.0
62	26178.0
63	18870.0
64	15622.5
65	12375.0
66	10387.5
67	8400.0
68	8400.0
69	6715.5
70	5031.0
71	4216.5
72	3402.0
73	2640.0
74	1878.0
75	1878.0
76	1481.0
77	1084.0
78	809.0
79	534.0
80	392.0
81	250.0
82	250.0
83	180.5
84	111.0
85	78.5
86	46.0
87	34.0
88	22.0
89	22.0
90	16.0
91	10.0
92	6.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	794359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.47407246324624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61225737015579	42.300534346674105
2	11.679958813916397	12.257900103383612
3	3.3329046435410827	5.246732393347939
4	1.3706159004874188	2.876871923258172
5	0.7128680807749114	1.8703545663658987
6	0.43126872778444225	1.3578255883735704
7	0.29246280056419705	1.0742699932726727
8	0.19257192494863867	0.8084026515313345
9	0.15990563357760498	0.7551809823269292
>10	0.9992391753157297	10.41670936689468
>50	0.1274089040194639	4.6768129560352225
>100	0.08277945418843133	7.856818909704276
>500	0.0033591662567863207	1.210213508514277
>1k	0.0014396426814798518	1.7020043911876817
>5k	4.798808938266173E-4	2.1642072407253337
>10k+	4.798808938266173E-4	3.425161078404327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	14238	1.7923885799745454	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	12966	1.6322594695849106	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	9913	1.2479244271167067	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	7276	0.9159586534551758	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	3358	0.42273078041540413	Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	3268	0.41140089052934503	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2030	0.2555519607633324	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.2481245885046937	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1714	0.21577145849672502	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	1177	0.14816978217657256	No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT	929	0.11694964115720977	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	905	0.11392833718759403	No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	868	0.10927049356776973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2588766540065638E-4	0.0
10	0.0	0.0	0.0	2.5177533080131276E-4	0.0
11	0.0	0.0	0.0	3.7766299620196913E-4	0.0
12	0.0	0.0	0.0	5.035506616026255E-4	0.0
13	0.0	0.0	0.0	5.035506616026255E-4	0.0
14	0.0	0.0	0.0	5.035506616026255E-4	0.0
15	0.0	0.0	0.0	6.294383270032819E-4	0.0
16	0.0	0.0	0.0	7.553259924039383E-4	0.0
17	0.0	0.0	0.0	7.553259924039383E-4	0.0
18	0.0	0.0	0.0	7.553259924039383E-4	0.0
19	0.0	0.0	0.0	8.812136578045947E-4	0.0
20	0.0	0.0	0.0	0.001007101323205251	0.0
21	0.0	0.0	0.0	0.0013847643194072202	0.0
22	0.0	0.0	0.0	0.0013847643194072202	0.0
23	0.0	0.0	0.0	0.002014202646410502	0.0
24	0.0	0.0	0.0	0.002391865642612471	0.0
25	0.0	0.0	0.0	0.0025177533080131278	0.0
26	0.0	0.0	0.0	0.0027695286388144404	0.0
27	0.0	0.0	0.0	0.0032730793004170657	0.0
28	0.0	0.0	0.0	0.00830858591644332	0.0
29	0.0	0.0	0.0	0.019512588137101737	0.0
30	0.0	0.0	0.0	0.0346191079851805	0.0
31	0.0	0.0	0.0	0.07679147589440039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1735	0.0	33.054756	1
TTATACA	1930	0.0	30.290155	2
GATTACG	55	1.8542778E-8	30.272728	1
TATACAC	1945	0.0	29.96144	3
CGTCTTC	445	0.0	28.685394	37
CCGTCTT	1605	0.0	26.856699	37
GACCGTT	35	8.866975E-4	26.42857	7
GCCGTCT	1645	0.0	26.091185	36
GGTATCA	425	0.0	25.682354	1
GTATCAA	1175	0.0	24.246809	1
ATTACGG	75	3.7377504E-7	22.2	2
TTACGGG	75	3.7377504E-7	22.2	3
TGCCGTC	2015	0.0	21.300247	35
ATGCCGT	2090	0.0	20.624401	34
CCGTTAA	45	0.0038247837	20.555555	16
TCGCACC	55	5.1411707E-4	20.181818	28
TATGCCG	2270	0.0	18.988987	33
TCGCCGA	60	9.2333916E-4	18.5	1
AAGTACG	60	9.2333916E-4	18.5	35
ACGCATC	50	0.007033018	18.499998	1
>>END_MODULE