FastQCFastQC Report
Fri 10 Feb 2017
ERR1630898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334471
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG104433.122243781972129No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT100643.00893052013478TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT88762.653742775905833No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC54531.6303356643774796No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC43991.315211184228229RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC29140.8712265039420458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.7229326309306338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21470.6419091640231889No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG11630.34771325466183917No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10260.30675305183409024No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10080.3013714193457729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8970.2681846856678158No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT8600.2571224411084967No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7370.22034795243832797No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7140.21347142203658911No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6990.20898672829632467No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6330.18925407583916093No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4850.14500509760188476No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4820.14410815885383185No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4530.1354377509559872No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4480.13394285304256573No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4210.12587040431008967No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4110.12288060848324668No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3620.10823060893171606No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3580.10703469060097887No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3410.10195203769534579No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.10105509894729289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTG200.001840071237.017
GCTCTAG403.819514E-837.01
CTAGTGT200.001840071237.018
GTGTCCA200.001840071237.021
ACAGACA251.2317547E-437.017
CGCCGAG251.2317547E-437.02
GATTACG403.819514E-837.01
CAGACAC251.2317547E-437.018
CTTATAC12050.034.850621
TTATACA13300.031.2969912
TCGCCGA303.594072E-430.8333321
GATACAC303.594072E-430.8333323
GCCGAGC303.594072E-430.8333323
CGTCTTC4500.030.42222237
CGTGGGT250.005490841329.627
AGCAACG250.005490841329.62
ATGGCTT250.005490841329.611
TGGGTAC250.005490841329.629
TCAGAGT502.71104E-729.63
AGTCTAC250.005490841329.635
Produced by FastQC (version 0.11.5)