FastQCFastQC Report
Fri 10 Feb 2017
ERR1630897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777696
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT169832.1837581780027158No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG129411.6640178167304447No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT90441.1629222729704152TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC70850.9110243591326175No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA38860.4996811093280665No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC32140.41327202402995517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32110.4128862691848743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.3831831461136485No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC28340.36440974365304696RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG17600.22630950911410116No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA11840.15224457885857712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10780.13861457433238694No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10260.13192815701765215No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT9790.12588466444471874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8710.11199749002180802No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT8250.10608258239723492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG700.037.01
CTTATAC20950.033.8210031
GGTATCA4450.031.5955051
CGTCTTC3950.031.37974537
TTATACA22850.030.68492
TATACAC24750.029.1515143
TTACGGG1100.026.909093
CCGTCTT13100.026.26717637
GTATCAA15550.025.6977481
GCCGTCT13600.025.30147236
ATTACGG1100.025.2272722
GCTTTAT6950.022.8920861
CCGGCGT502.7010127E-422.223
GGACCGT502.7010127E-422.26
GCTACCG450.003824711320.5555577
TTATTGA7850.020.2675154
CGGCGTC555.14104E-420.18181824
GCGTCAA555.14104E-420.18181826
ATTGAGC7550.020.0927146
TGCCGTC17200.020.00581435
Produced by FastQC (version 0.11.5)