##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630897.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	777696
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.464541414640166	31.0	31.0	34.0	28.0	34.0
2	31.46832566967041	31.0	31.0	34.0	28.0	34.0
3	30.948059395959348	31.0	31.0	34.0	27.0	34.0
4	35.09341182158581	37.0	35.0	37.0	32.0	37.0
5	35.2959768341357	37.0	35.0	37.0	33.0	37.0
6	35.48667474180142	37.0	35.0	37.0	33.0	37.0
7	35.488544366950585	37.0	35.0	37.0	33.0	37.0
8	35.50833230465375	37.0	35.0	37.0	33.0	37.0
9	37.287582808706745	39.0	37.0	39.0	34.0	39.0
10	37.11638223676089	39.0	37.0	39.0	33.0	39.0
11	37.170967833189316	39.0	37.0	39.0	33.0	39.0
12	37.101238787392504	39.0	37.0	39.0	33.0	39.0
13	37.17717462864667	39.0	37.0	39.0	33.0	39.0
14	38.28402229148665	40.0	38.0	41.0	33.0	41.0
15	38.29607198699749	40.0	38.0	41.0	34.0	41.0
16	38.29606555775007	40.0	38.0	41.0	33.0	41.0
17	38.28191735588199	40.0	38.0	41.0	33.0	41.0
18	38.31593578982019	40.0	38.0	41.0	34.0	41.0
19	38.35029497387154	40.0	38.0	41.0	34.0	41.0
20	38.340228058264415	40.0	38.0	41.0	34.0	41.0
21	38.307773217298276	40.0	38.0	41.0	34.0	41.0
22	38.219315773772784	40.0	38.0	41.0	34.0	41.0
23	38.2352808809612	40.0	38.0	41.0	34.0	41.0
24	38.20672216393038	40.0	38.0	41.0	33.0	41.0
25	38.178271458256184	40.0	38.0	41.0	33.0	41.0
26	38.09417175863062	40.0	37.0	41.0	33.0	41.0
27	37.9934755997202	40.0	37.0	41.0	33.0	41.0
28	37.99601129490186	40.0	37.0	41.0	33.0	41.0
29	37.92534872237995	40.0	37.0	41.0	33.0	41.0
30	37.84310578940872	40.0	37.0	41.0	33.0	41.0
31	37.832266078261945	40.0	37.0	41.0	33.0	41.0
32	37.71384191252109	40.0	37.0	41.0	33.0	41.0
33	37.6870923857137	40.0	37.0	41.0	33.0	41.0
34	37.633831214253384	40.0	37.0	41.0	33.0	41.0
35	37.613276910257994	40.0	37.0	41.0	33.0	41.0
36	37.5828421388306	40.0	37.0	41.0	32.0	41.0
37	37.49899703740279	40.0	37.0	41.0	32.0	41.0
38	37.464045076739495	40.0	37.0	41.0	32.0	41.0
39	37.37046095132288	39.0	36.0	41.0	32.0	41.0
40	37.2735336172489	39.0	36.0	41.0	31.0	41.0
41	37.239520326708636	39.0	36.0	41.0	31.0	41.0
42	37.169015913673206	39.0	36.0	41.0	31.0	41.0
43	36.485138151668515	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	18.0
12	12.0
13	16.0
14	11.0
15	9.0
16	17.0
17	11.0
18	25.0
19	36.0
20	72.0
21	124.0
22	279.0
23	502.0
24	1013.0
25	1691.0
26	2798.0
27	4267.0
28	6427.0
29	9055.0
30	12585.0
31	16834.0
32	22471.0
33	28883.0
34	38306.0
35	51881.0
36	72227.0
37	107523.0
38	185457.0
39	215129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.20143089330536	22.591475332263506	15.262776200469078	23.944317573962064
2	13.454871826523473	22.133584331152534	41.12969592231412	23.281847920009877
3	14.338764761552072	28.687430564127887	35.244748590708966	21.729056083611077
4	10.88741616261367	19.320145661029503	39.19282084516315	30.59961733119368
5	11.98064539357281	38.41796794634407	36.398412747397444	13.202973912685675
6	26.595867794099494	41.462730938567255	17.343795004731927	14.597606262601326
7	23.681747109410363	33.585359832119494	23.00359009175822	19.729302966711927
8	24.835282681150474	34.54357486730033	22.807883800353864	17.813258651195326
9	24.130122824342674	14.526113031313006	19.783951569765048	41.55981257457927
10	14.290159651071885	29.44916779821421	33.6717689174176	22.5889036332963
11	31.104699008352878	23.28429103402872	25.667227297041517	19.943782660576883
12	20.595965518660247	26.9510194214706	28.592920627083075	23.860094432786074
13	29.08360079002592	20.57899230547669	26.935332057770644	23.40207484672674
14	21.122778052092333	22.51651030736946	27.39438032341686	28.96633131712134
15	23.828591120437807	29.198041394066575	22.884134674731516	24.089232810764102
16	24.112892441262396	27.3324023783072	24.31747109410361	24.237234086326794
17	20.98943546064272	30.761634366127637	25.02378821544665	23.22514195778299
18	23.806988849113278	25.409414475579144	27.098634942188205	23.684961733119366
19	24.045128173476527	27.053244455417026	27.477060445212526	21.424566925893924
20	23.47048203925441	26.912829691807595	25.562687734024607	24.054000534913385
21	25.301274534008144	25.45609081183393	26.316838456157676	22.92579619800025
22	21.00718018351644	31.071781261572646	26.538647492079164	21.382391062831747
23	24.509962761798953	24.971325556515655	26.595224869357693	23.923486812327695
24	22.39795498498128	27.251008105995144	27.61066020655886	22.740376702464715
25	21.777018269349462	29.069456445706294	27.11766551454553	22.035859770398716
26	24.333544212648643	26.12203225939184	27.539552730115624	22.004870797843886
27	20.98879253590092	30.46408879562194	26.74682652347447	21.800292145002675
28	23.393459655186604	25.445675431016745	27.750303460478133	23.410561453318522
29	20.919099493889643	26.347055919022345	28.794927580957086	23.93891700613093
30	23.32415236802041	27.813695840019754	28.00580175287002	20.856350039089826
31	23.721994198247128	25.52964140229601	25.94689955972514	24.80146483973172
32	21.355002468831007	26.637657902316587	29.006064066164672	23.001275562687734
33	22.070320536559272	26.163822367608937	28.895738180471547	22.870118915360244
34	21.547494136526353	27.45687260831996	26.977122165987737	24.018511089165948
35	20.558675883635765	25.55677282640003	32.763702012097276	21.12084927786693
36	22.157372546599184	25.993833065876643	26.353099411595277	25.495694975928895
37	21.818422622721474	26.910386577788753	29.1351633543184	22.136027445171376
38	21.95639427231206	25.031246142451547	29.43489486894622	23.57746471629017
39	23.971834752911164	25.750036003785542	27.084490597868577	23.19363864543472
40	20.793471999341644	24.850970044850428	32.61827243550179	21.737285520306134
41	21.77483232522734	26.012735053285603	26.776401061597333	25.436031559889727
42	22.368637616755134	25.135528535571737	30.060460642719004	22.43537320495412
43	22.554442867135744	25.09463852199317	30.134268403077808	22.216650207793275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	26.0
2	39.0
3	141.0
4	243.0
5	243.0
6	303.5
7	364.0
8	350.0
9	336.0
10	542.0
11	748.0
12	748.0
13	1064.5
14	1381.0
15	3168.0
16	4955.0
17	5747.5
18	6540.0
19	6540.0
20	6822.5
21	7105.0
22	8841.5
23	10578.0
24	13538.5
25	16499.0
26	16499.0
27	19325.0
28	22151.0
29	25409.0
30	28667.0
31	32310.5
32	35954.0
33	35954.0
34	38568.5
35	41183.0
36	43942.5
37	46702.0
38	47903.5
39	49105.0
40	49105.0
41	49953.0
42	50801.0
43	50915.0
44	51029.0
45	50970.5
46	50912.0
47	50912.0
48	50650.5
49	50389.0
50	50040.5
51	49692.0
52	62211.0
53	74730.0
54	74730.0
55	58519.5
56	42309.0
57	44956.5
58	47604.0
59	40550.5
60	33497.0
61	33497.0
62	26657.5
63	19818.0
64	16951.0
65	14084.0
66	11472.5
67	8861.0
68	8861.0
69	7195.0
70	5529.0
71	4673.5
72	3818.0
73	2516.5
74	1215.0
75	1215.0
76	828.5
77	442.0
78	326.0
79	210.0
80	154.0
81	98.0
82	98.0
83	70.5
84	43.0
85	38.0
86	33.0
87	24.5
88	16.0
89	16.0
90	9.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	777696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.57412930541714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53975955386204	39.121486947965
2	11.666655547591981	11.333952702609903
3	3.2036270361910915	4.6684018170682915
4	1.2406811687577042	2.4106003007213115
5	0.6184459903426799	1.5020237751661047
6	0.4445104974537293	1.2955026228559647
7	0.30261387485960434	1.028944384093062
8	0.2177358454860681	0.8461063290451679
9	0.17392161643102197	0.7603281976974862
>10	1.3196535840529833	12.950548870834306
>50	0.16157698082131075	5.417115065686818
>100	0.10417452092738014	8.934069337657547
>500	0.003189015946747791	1.0529577169072926
>1k	0.0023917619600608434	2.7331462228856327
>5k	5.315026577912985E-4	2.082034450900787
>10k+	5.315026577912985E-4	3.8627812579053358
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	16983	2.1837581780027158	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	12941	1.6640178167304447	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	9044	1.1629222729704152	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	7085	0.9110243591326175	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3886	0.4996811093280665	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	3214	0.41327202402995517	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3211	0.4128862691848743	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2980	0.3831831461136485	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2834	0.36440974365304696	RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1760	0.22630950911410116	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1184	0.15224457885857712	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1078	0.13861457433238694	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1026	0.13192815701765215	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	979	0.12588466444471874	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	871	0.11199749002180802	No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT	825	0.10608258239723492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.2858494836028473E-4	0.0
12	0.0	0.0	0.0	1.2858494836028473E-4	0.0
13	0.0	0.0	0.0	1.2858494836028473E-4	0.0
14	0.0	0.0	0.0	1.2858494836028473E-4	0.0
15	0.0	0.0	0.0	1.2858494836028473E-4	0.0
16	0.0	0.0	0.0	1.2858494836028473E-4	0.0
17	0.0	0.0	0.0	2.5716989672056945E-4	0.0
18	0.0	0.0	0.0	2.5716989672056945E-4	0.0
19	0.0	0.0	0.0	2.5716989672056945E-4	0.0
20	0.0	0.0	0.0	6.429247418014237E-4	0.0
21	0.0	0.0	0.0	0.0011572645352425626	0.0
22	0.0	0.0	0.0	0.0014144344319631322	0.0
23	0.0	0.0	0.0	0.0020573591737645556	0.0
24	0.0	0.0	0.0	0.0025716989672056947	0.0
25	0.0	0.0	0.0	0.0025716989672056947	0.0
26	0.0	0.0	0.0	0.0028288688639262643	0.0
27	0.0	0.0	0.0	0.0036003785540879726	0.0
28	0.0	0.0	0.0	0.00951528617866107	0.0
29	0.0	0.0	0.0	0.024816895033534955	0.0
30	0.0	0.0	0.0	0.04320454264905567	0.0
31	0.0	0.0	0.0	0.09450993704480928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	70	0.0	37.0	1
CTTATAC	2095	0.0	33.821003	1
GGTATCA	445	0.0	31.595505	1
CGTCTTC	395	0.0	31.379745	37
TTATACA	2285	0.0	30.6849	2
TATACAC	2475	0.0	29.151514	3
TTACGGG	110	0.0	26.90909	3
CCGTCTT	1310	0.0	26.267176	37
GTATCAA	1555	0.0	25.697748	1
GCCGTCT	1360	0.0	25.301472	36
ATTACGG	110	0.0	25.227272	2
GCTTTAT	695	0.0	22.892086	1
CCGGCGT	50	2.7010127E-4	22.2	23
GGACCGT	50	2.7010127E-4	22.2	6
GCTACCG	45	0.0038247113	20.555557	7
TTATTGA	785	0.0	20.267515	4
CGGCGTC	55	5.14104E-4	20.181818	24
GCGTCAA	55	5.14104E-4	20.181818	26
ATTGAGC	755	0.0	20.092714	6
TGCCGTC	1720	0.0	20.005814	35
>>END_MODULE