FastQCFastQC Report
Fri 10 Feb 2017
ERR1630896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728499
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT163262.241046315780804No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT132891.824161735294077RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG125621.7243675008476333No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC64770.8890883858454164No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC58680.80549184007116RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC35360.4853815859733507RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.44186745623535517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31900.43788666834134293No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15140.20782458177705118No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG14550.19972573744095737No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA12970.1780373068459943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11930.16376137784677808No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT10840.14879910610721497No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8660.11887456262808871No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT7780.10679493039798271No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7710.10583405056149699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19550.033.4987221
GACGTAT303.5981956E-430.83333428
ACGTATA303.5981956E-430.83333429
TTATACA21400.030.4299052
CGTCTTC7550.030.13907437
TATACAC21900.029.9041123
GATCGGA250.005495037429.611
TTACGGG502.7184615E-729.63
GATTACG502.7184615E-729.61
GCCTTAC405.93719E-527.751
GGTATCA5900.026.3389841
CTTACTC601.3362842E-624.6666683
CCGTCTT21750.023.98620637
GCCGTCT21900.023.73744436
GTATCAA14750.022.3254241
TGCCGTC25050.020.75249735
TCGCCGA450.00382448420.5555551
CCTAAGC450.00382448420.5555552
GCCGAGC450.00382448420.5555553
ATGCCGT26200.019.84160434
Produced by FastQC (version 0.11.5)