##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630896.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728499
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446173570588293	31.0	31.0	34.0	28.0	34.0
2	31.452051409816622	31.0	31.0	34.0	28.0	34.0
3	30.923848900273025	31.0	31.0	34.0	27.0	34.0
4	35.068125007721356	37.0	35.0	37.0	32.0	37.0
5	35.26492555240296	37.0	35.0	37.0	33.0	37.0
6	35.45151057173723	37.0	35.0	37.0	33.0	37.0
7	35.45662519783829	37.0	35.0	37.0	33.0	37.0
8	35.48054836039583	37.0	35.0	37.0	33.0	37.0
9	37.25828587273284	39.0	37.0	39.0	34.0	39.0
10	37.095521064545046	39.0	37.0	39.0	33.0	39.0
11	37.15005785869301	39.0	37.0	39.0	33.0	39.0
12	37.075279444446736	39.0	37.0	39.0	33.0	39.0
13	37.15014021982185	39.0	37.0	39.0	33.0	39.0
14	38.23981089884818	40.0	38.0	41.0	33.0	41.0
15	38.24631468265571	40.0	38.0	41.0	33.0	41.0
16	38.24608681686591	40.0	38.0	41.0	33.0	41.0
17	38.21607030345958	40.0	38.0	41.0	33.0	41.0
18	38.287518582729696	40.0	38.0	41.0	33.0	41.0
19	38.313686086048165	40.0	38.0	41.0	34.0	41.0
20	38.315149368770584	40.0	38.0	41.0	34.0	41.0
21	38.26917950470762	40.0	38.0	41.0	34.0	41.0
22	38.19357610648745	40.0	38.0	41.0	34.0	41.0
23	38.18348961357531	40.0	38.0	41.0	34.0	41.0
24	38.143565056369326	40.0	37.0	41.0	33.0	41.0
25	38.09655057865557	40.0	37.0	41.0	33.0	41.0
26	37.99438159832752	40.0	37.0	41.0	33.0	41.0
27	37.91261621498451	40.0	37.0	41.0	33.0	41.0
28	37.911718478680136	40.0	37.0	41.0	33.0	41.0
29	37.863607225267295	40.0	37.0	41.0	33.0	41.0
30	37.77715274832224	40.0	37.0	41.0	33.0	41.0
31	37.73931604573239	40.0	37.0	41.0	33.0	41.0
32	37.62170160837558	40.0	37.0	41.0	33.0	41.0
33	37.57832200181469	40.0	37.0	41.0	32.0	41.0
34	37.51412150188264	40.0	37.0	41.0	32.0	41.0
35	37.47787436907944	40.0	37.0	41.0	32.0	41.0
36	37.43287773902229	39.0	36.0	41.0	32.0	41.0
37	37.37857979214797	39.0	36.0	41.0	32.0	41.0
38	37.334922903119974	39.0	36.0	41.0	32.0	41.0
39	37.21044366567421	39.0	36.0	41.0	31.0	41.0
40	37.10706123138124	39.0	36.0	41.0	31.0	41.0
41	37.052805837756814	39.0	36.0	41.0	31.0	41.0
42	36.980662979633465	39.0	36.0	41.0	31.0	41.0
43	36.30928388371158	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	13.0
11	23.0
12	22.0
13	16.0
14	16.0
15	17.0
16	12.0
17	14.0
18	23.0
19	36.0
20	73.0
21	172.0
22	309.0
23	561.0
24	1061.0
25	1656.0
26	2736.0
27	4391.0
28	6522.0
29	9128.0
30	12476.0
31	16530.0
32	21498.0
33	27786.0
34	36733.0
35	49384.0
36	67489.0
37	101054.0
38	173276.0
39	195461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.28117677580889	22.59412847512488	15.66934203066854	24.45535271839769
2	13.254239195935751	22.1039424899691	41.71783351795953	22.923984796135617
3	14.25286788314054	28.790018929332778	35.06538787287285	21.89172531465383
4	11.000426905184495	19.634344041652767	37.6501546330194	31.715074420143335
5	12.430216101875226	38.064019305448596	35.576713214431315	13.929051378244855
6	26.98315303109544	40.763130766136946	17.501190804654502	14.752525398113105
7	23.938673903464522	33.71727346228341	22.38314671674223	19.960905917509837
8	24.960363706744964	34.653170423020484	22.635720845189905	17.750745025044644
9	23.337986737112885	14.54168090827853	19.81526398800822	42.305068366600366
10	14.13440512615666	28.92234580967167	33.33649050993893	23.606758554232744
11	30.34280074509368	23.18657952859235	25.54746128683773	20.92315843947624
12	19.746766982521596	26.998115302835007	29.192902117916432	24.06221559672697
13	29.177939846176866	20.71684381172795	26.94554144892443	23.159674893170752
14	21.507922454251823	22.971067908123416	27.081574580061197	28.439435057563568
15	23.88761000358271	29.002647910292257	23.011287592707745	24.098454493417286
16	24.588091404380787	27.379172792275625	23.794541927991663	24.23819387535192
17	19.944982765933787	31.35776438951872	25.143754486965662	23.553498357581823
18	23.35747887093874	25.083768131459344	26.112733167787468	25.446019829814453
19	22.693373635379047	26.873338192639935	26.951306727943347	23.48198144403767
20	21.689528743347623	25.345264715531524	27.972310188483444	24.99289635263741
21	23.40634647405144	25.019663719510937	26.87690717488974	24.697082631547882
22	20.08307492529159	30.035044660322114	28.759819848757513	21.12206056562878
23	22.06138924006759	24.805799321618835	27.479241563818206	25.653569874495368
24	22.223915200981743	27.686242534306842	28.545269108125066	21.54457315658635
25	20.47278033326058	29.513561446206516	27.09255606390675	22.92110215662616
26	22.681705808793147	26.177112116832006	26.46029713149915	24.680884942875693
27	20.202910367756168	29.76297839804859	29.51548320587949	20.518628028315756
28	19.78616305581751	25.254667473805732	28.452338301082087	26.506831169294674
29	20.302704602202613	25.535381654607626	29.606354984701422	24.555558758488345
30	19.99632120291174	26.047942413098713	31.03079070801744	22.924945675972104
31	21.68074355627118	24.664412717107368	28.03339469237432	25.621449034247128
32	21.351710846548862	27.613902009474273	30.273617396866708	20.760769747110153
33	19.975044577960986	25.797427312872085	29.590020027481163	24.63750808168577
34	20.34553238920026	28.359270225491045	29.629553369325144	21.66564401598355
35	20.674565098922578	27.842454142009803	31.245204180101826	20.23777657896579
36	22.58767685336562	25.709712710655747	25.875395848175497	25.827214587803137
37	21.56502617024869	27.06963221637916	28.352544066635645	23.012797546736508
38	21.74141625451785	24.873472715817044	29.036278704569256	24.34883232509585
39	23.765303727252885	25.455628628179312	27.55501380235251	23.224053842215294
40	20.2180099080438	24.955696576110604	33.02091011792741	21.805383397918185
41	21.941416529054948	25.692416873599004	27.16736742260456	25.198799174741488
42	22.410463157808042	24.84080280137653	31.04987103619909	21.698863004616342
43	22.370792547415988	24.517809907769262	30.635320021029543	22.476077523785207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	18.0
2	27.0
3	124.5
4	222.0
5	222.0
6	278.0
7	334.0
8	352.5
9	371.0
10	549.5
11	728.0
12	728.0
13	1156.5
14	1585.0
15	3514.0
16	5443.0
17	6066.5
18	6690.0
19	6690.0
20	6992.5
21	7295.0
22	8391.5
23	9488.0
24	12013.0
25	14538.0
26	14538.0
27	17582.0
28	20626.0
29	23543.5
30	26461.0
31	29520.5
32	32580.0
33	32580.0
34	35588.5
35	38597.0
36	40376.0
37	42155.0
38	43443.0
39	44731.0
40	44731.0
41	45237.0
42	45743.0
43	46359.0
44	46975.0
45	47532.5
46	48090.0
47	48090.0
48	49599.0
49	51108.0
50	62978.5
51	74849.0
52	60027.5
53	45206.0
54	45206.0
55	52730.0
56	60254.0
57	51015.0
58	41776.0
59	31630.5
60	21485.0
61	21485.0
62	18598.5
63	15712.0
64	12910.0
65	10108.0
66	8425.5
67	6743.0
68	6743.0
69	5379.5
70	4016.0
71	3121.5
72	2227.0
73	1700.0
74	1173.0
75	1173.0
76	872.0
77	571.0
78	444.5
79	318.0
80	231.5
81	145.0
82	145.0
83	104.5
84	64.0
85	50.5
86	37.0
87	25.5
88	14.0
89	14.0
90	9.5
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	728499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.67657556508794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.77075227302046	44.16268140104801
2	11.870876690615065	12.981177727965113
3	3.2022889818612765	5.252705864939598
4	1.2545748159027732	2.7438341889505704
5	0.6595427277123792	1.803076889508506
6	0.40261059936511895	1.3208021315695364
7	0.27663186455820826	1.0587698132359629
8	0.2128376620983169	0.9309787611852219
9	0.15378396503864966	0.7567542526621113
>10	1.0590542277396993	11.194907709635253
>50	0.09211719237041252	3.453879629142067
>100	0.039155999754005265	3.7039218825055378
>500	0.002509999984197665	0.8912248955054695
>1k	0.0020079999873581318	2.262783504326175
>5k	5.019999968395329E-4	1.6942056259647398
>10k+	7.529999952592995E-4	5.788295721856203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	16326	2.241046315780804	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	13289	1.824161735294077	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	12562	1.7243675008476333	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	6477	0.8890883858454164	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	5868	0.80549184007116	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3536	0.4853815859733507	RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3219	0.44186745623535517	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3190	0.43788666834134293	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1514	0.20782458177705118	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1455	0.19972573744095737	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1297	0.1780373068459943	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.16376137784677808	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1084	0.14879910610721497	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	866	0.11887456262808871	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	778	0.10679493039798271	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	771	0.10583405056149699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.372685480693865E-4	0.0
10	0.0	0.0	0.0	1.372685480693865E-4	0.0
11	0.0	0.0	0.0	1.372685480693865E-4	0.0
12	0.0	0.0	0.0	1.372685480693865E-4	0.0
13	0.0	0.0	0.0	1.372685480693865E-4	0.0
14	0.0	0.0	0.0	1.372685480693865E-4	0.0
15	0.0	0.0	0.0	4.1180564420815953E-4	0.0
16	0.0	0.0	0.0	4.1180564420815953E-4	0.0
17	0.0	0.0	0.0	4.1180564420815953E-4	0.0
18	0.0	0.0	0.0	5.49074192277546E-4	0.0
19	0.0	0.0	0.0	5.49074192277546E-4	0.0
20	0.0	0.0	0.0	6.863427403469326E-4	0.0
21	0.0	0.0	0.0	6.863427403469326E-4	0.0
22	0.0	0.0	0.0	0.0015099540287632515	0.0
23	0.0	0.0	0.0	0.0020590282210407977	0.0
24	0.0	0.0	0.0	0.002470833865248957	0.0
25	0.0	0.0	0.0	0.0026081024133183437	0.0
26	0.0	0.0	0.0	0.0031571766055958897	0.0
27	0.0	0.0	0.0	0.003568982249804049	0.0
28	0.0	0.0	0.0	0.009883335460995828	0.0
29	0.0	0.0	0.0	0.021688430594963067	0.0
30	0.0	0.0	0.0	0.03747431362294252	0.0
31	0.0	0.0	0.0	0.08689099092792166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1955	0.0	33.498722	1
GACGTAT	30	3.5981956E-4	30.833334	28
ACGTATA	30	3.5981956E-4	30.833334	29
TTATACA	2140	0.0	30.429905	2
CGTCTTC	755	0.0	30.139074	37
TATACAC	2190	0.0	29.904112	3
GATCGGA	25	0.0054950374	29.6	11
TTACGGG	50	2.7184615E-7	29.6	3
GATTACG	50	2.7184615E-7	29.6	1
GCCTTAC	40	5.93719E-5	27.75	1
GGTATCA	590	0.0	26.338984	1
CTTACTC	60	1.3362842E-6	24.666668	3
CCGTCTT	2175	0.0	23.986206	37
GCCGTCT	2190	0.0	23.737444	36
GTATCAA	1475	0.0	22.325424	1
TGCCGTC	2505	0.0	20.752497	35
TCGCCGA	45	0.003824484	20.555555	1
CCTAAGC	45	0.003824484	20.555555	2
GCCGAGC	45	0.003824484	20.555555	3
ATGCCGT	2620	0.0	19.841604	34
>>END_MODULE