FastQCFastQC Report
Fri 10 Feb 2017
ERR1630895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149948
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG139359.29322165017206No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT106577.107130471896925No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT105067.006428895350387RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC76885.12711073172033No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC38172.545549123696215No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC37972.532211166537733RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG17941.1964147571158001No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA11520.7682663323285406No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT11330.755595273027983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.7362552351481847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9720.6482247179022061No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9140.6095446421426095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3690.24608530957398564No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT3330.22207698668871875No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2920.19473417451383146No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2760.18406380878704617No Hit
CTGATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT2340.15605409875423482No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2150.14338303945367728No Hit
CTTTACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA2000.13337957158481606No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1720.11470643156294182No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1670.11137194227332141No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA1640.10937124869954919No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1560.10403606583615653No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1540.10270227012030837No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG1510.10070157654653612No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAAT1510.10070157654653612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTT200.00183683537.02
TCACTAG200.00183683537.016
TCGTACT200.00183683537.026
ATGCCCC200.00183683537.09
CTTATAC12800.035.2656251
ACAGACA603.274181E-1133.91666817
CAGACAC603.274181E-1133.91666818
GACAGAC554.9294613E-1033.63636416
AGACAGA554.9294613E-1033.63636415
TTATACA13000.033.5846142
CGTCTTC4100.033.39024737
TGATACA352.3745788E-531.7142872
ATGTGTA705.456968E-1231.7142874
TATAAGA658.54925E-1131.3076929
TATACAC15000.029.7233313
ATCGTAC250.005481297629.59999825
AACTCAT250.005481297629.59999810
TGTATAA702.0372681E-1029.071437
GTGTATA702.0372681E-1029.071436
TCACGCC453.977768E-628.77777729
Produced by FastQC (version 0.11.5)