FastQCFastQC Report
Fri 10 Feb 2017
ERR1630894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725804
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT133511.8394773244567404No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG107511.4812538922353693No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT98861.3620757119001825TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC54750.7543358813123102No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC36920.5086772737543469RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC30060.41416139894516973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.3878457545012152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27670.38123239882943605No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG12580.17332502989787876No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA11130.15334718463937924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10660.14687160721076215No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9510.13102710924712457No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT9270.12772043141123499No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9210.1268937619522626No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT7270.10016478277882182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC200.001841489337.07
CTTATAC15550.032.122191
CGTCTTC5050.030.40594137
GATTACG551.8537321E-830.2727281
TTATACA17150.029.6647222
ACCGTCG250.00549502629.5999988
TATACAC19100.027.4109943
CCGTCTT16050.025.81931337
GCCGTCT16200.025.58024636
TTCGGTC451.3225555E-424.66666828
CGCCGAG451.3225555E-424.6666682
GGTATCA5100.024.6666661
TTACGGG701.9208892E-723.7857133
GTATCAA13700.023.496351
TCGCCGA502.7007732E-422.1999991
GCCGAGC502.7007732E-422.1999993
TGCCGTC19500.021.1564135
ATGCCGT19950.020.67919734
CGTCCGC450.003824469220.55555710
TTTTCGG450.003824469220.55555726
Produced by FastQC (version 0.11.5)