FastQCFastQC Report
Fri 10 Feb 2017
ERR1630893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620305
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT112851.8192663286609005No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG99331.6013090334593465No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT75201.2123068490500641Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC54300.8753758231837563No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG38990.6285617559103989No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC25950.41834258953256864No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC25240.40689660731414384Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20400.32887047500826205No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA19820.3195202360129291No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG19660.3169408597383545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.31355542837797534No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC14460.23311113081467988No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14150.22811358928269157No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG13910.22424452487082966No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT11730.18910052312975068No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG10180.16411281546980921No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC10180.16411281546980921No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT9910.15976011800646456No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA9900.15959890698930365No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC9640.1554074205431199No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA9170.147830502736557No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC9110.14686323663359155No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT8750.14105964001579868No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT8620.1389638967927068No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG8430.13590088746664947No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8160.1315481900033048No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA8080.1302585018660175No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT7290.1175228315103054No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT7290.1175228315103054No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7130.11494345523573081No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT7020.11317013404696077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6790.10946228065225978No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA6740.10865622556645521No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC6740.10865622556645521No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC6710.10817259251497248No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC6400.10317505098298418No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA6290.10140172979421413No Hit
GTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGG6250.1007568857255705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG750.034.5333331
CTTATAC12850.032.9688721
CGTCTTC3700.031.537
TCGAGTA250.005494417629.5999982
TATACAC14700.029.3231283
TTATACA14950.028.5852872
TGCGTTA358.8647753E-426.4285737
TTACGGG1000.025.8999983
GTATCAA10700.025.7616831
ATTACGG1051.8189894E-1224.6666662
CCGTCTT10250.024.18536637
GCCGTCT10350.023.95168936
GGTATCA3100.023.8709661
AACCACG951.6730155E-719.47368435
TGTGCTA951.6730155E-719.47368426
GTGCTAG1052.2544555E-819.3809527
ATAGGAC701.2180497E-418.53
GCGAACT609.2302816E-418.528
CTATTAA1306.9485395E-1018.51
CCGTGGC500.007031321518.4999989
Produced by FastQC (version 0.11.5)