##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630893.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620305
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45921764293372	31.0	31.0	34.0	28.0	34.0
2	31.45260637911995	31.0	31.0	34.0	28.0	34.0
3	30.935541386898382	31.0	31.0	34.0	27.0	34.0
4	35.09264797156238	37.0	35.0	37.0	32.0	37.0
5	35.28343476193163	37.0	35.0	37.0	33.0	37.0
6	35.47507758280201	37.0	35.0	37.0	33.0	37.0
7	35.47173406630609	37.0	35.0	37.0	33.0	37.0
8	35.49321382223261	37.0	35.0	37.0	33.0	37.0
9	37.28341058027905	39.0	37.0	39.0	34.0	39.0
10	37.10042640314039	39.0	37.0	39.0	33.0	39.0
11	37.15977785121835	39.0	37.0	39.0	33.0	39.0
12	37.06714116442718	39.0	37.0	39.0	33.0	39.0
13	37.1530746971248	39.0	37.0	39.0	33.0	39.0
14	38.29827746028164	40.0	38.0	41.0	33.0	41.0
15	38.30273333279597	40.0	38.0	41.0	33.0	41.0
16	38.2964283699148	40.0	38.0	41.0	33.0	41.0
17	38.25030751001523	40.0	38.0	41.0	33.0	41.0
18	38.267640918580376	40.0	38.0	41.0	33.0	41.0
19	38.3202940488953	40.0	38.0	41.0	34.0	41.0
20	38.30924948210961	40.0	38.0	41.0	34.0	41.0
21	38.28132612182716	40.0	38.0	41.0	34.0	41.0
22	38.195610224002706	40.0	38.0	41.0	33.0	41.0
23	38.20585034781277	40.0	38.0	41.0	34.0	41.0
24	38.16743053820298	40.0	38.0	41.0	33.0	41.0
25	38.10601720121553	40.0	37.0	41.0	33.0	41.0
26	38.04372203996421	40.0	37.0	41.0	33.0	41.0
27	37.95354382118474	40.0	37.0	41.0	33.0	41.0
28	37.93269440033532	40.0	37.0	41.0	33.0	41.0
29	37.879241663375275	40.0	37.0	41.0	33.0	41.0
30	37.79898598270206	40.0	37.0	41.0	33.0	41.0
31	37.77448029598343	40.0	37.0	41.0	33.0	41.0
32	37.670123568244655	40.0	37.0	41.0	32.0	41.0
33	37.63787169215144	40.0	37.0	41.0	32.0	41.0
34	37.55886539686122	40.0	37.0	41.0	32.0	41.0
35	37.547171149676366	40.0	37.0	41.0	32.0	41.0
36	37.529473404212446	40.0	37.0	41.0	32.0	41.0
37	37.46595465134087	39.0	36.0	41.0	32.0	41.0
38	37.43067684445555	39.0	36.0	41.0	32.0	41.0
39	37.3194025519704	39.0	36.0	41.0	31.0	41.0
40	37.215677771418896	39.0	36.0	41.0	31.0	41.0
41	37.15661327895148	39.0	36.0	41.0	31.0	41.0
42	37.08552083249369	39.0	36.0	41.0	31.0	41.0
43	36.42280329837741	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	10.0
11	15.0
12	10.0
13	5.0
14	10.0
15	9.0
16	5.0
17	8.0
18	13.0
19	27.0
20	69.0
21	126.0
22	216.0
23	438.0
24	805.0
25	1446.0
26	2266.0
27	3474.0
28	5110.0
29	7520.0
30	10274.0
31	13644.0
32	17970.0
33	23270.0
34	30886.0
35	42032.0
36	58986.0
37	86577.0
38	147069.0
39	168007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44172624757176	22.556806732172078	14.560095436922161	23.441371583334007
2	14.262499899243114	22.492805958359195	40.79589879172343	22.448795350674263
3	14.859947928841457	28.06941746398949	33.78257470115508	23.288059906013977
4	11.232377620686599	19.283578239736904	37.61423815703565	31.869805982540843
5	12.816275864292564	37.459475580561175	35.93490299127042	13.789345563875836
6	28.331868999927458	39.54103223414288	17.559748833235265	14.567349932694402
7	26.220971941222466	32.329579803483774	21.949202408492596	19.50024584680117
8	25.170359742384797	34.54735976656645	22.297095783525847	17.98518470752291
9	23.75121915831728	14.637960358210881	19.635340679182015	41.975479804289826
10	14.64650454212041	28.76488179202167	33.66311733743884	22.925496328419083
11	31.98458822675942	23.352383101861182	24.429756329547562	20.23327234183184
12	20.847325106197758	25.656088537090625	29.53160138963897	23.96498496707265
13	29.002829253351177	20.617599406743455	26.901927277710158	23.47764406219521
14	21.905191800807668	22.164096694368094	26.18679520558435	29.743916299239892
15	23.52165466988014	29.063767017838	23.396877342597595	24.01770096968427
16	25.798437865243713	27.20935668743602	22.86971731648141	24.122488130838864
17	22.36174140140737	29.65379934064694	24.23001587928519	23.754443378660497
18	23.553574451278	24.766042511345226	26.88757949718284	24.792803540193937
19	24.681406727335748	27.29576579263427	26.562900508620757	21.459926971409228
20	23.098798171867067	27.69008794060986	25.01462989980735	24.19648398771572
21	24.576780777198316	25.69252222696899	25.15778528304624	24.572911712786453
22	23.65610465819234	28.98960994994398	25.533084530996845	21.82120086086683
23	22.657563617897647	24.99125430231902	26.851145807304473	25.50003627247886
24	23.74299739644207	27.94560740280991	27.142776537348563	21.168618663399457
25	22.889062638540718	28.632527546932558	25.631584462482166	22.846825352044558
26	25.03494248796963	25.164072512715517	26.441508612698595	23.35947638661626
27	22.90873038263435	29.65428297369842	24.93354075817541	22.503445885491814
28	21.530537396925705	24.65883718493322	27.605613367617543	26.205012050523536
29	24.044461998532977	26.283683026898057	28.381199571178694	21.290655403390268
30	22.825061864727836	27.305277242646763	26.149233038585855	23.720427854039546
31	22.30644602252118	24.135385012211735	27.13100813309581	26.42716083217127
32	25.57387091833856	26.082008044429756	26.2694964573879	22.07462457984379
33	21.82635961341598	24.582423162798946	28.075704693658764	25.515512530126312
34	22.897123189398762	27.229669275598294	28.100692401318707	21.772515133684237
35	24.007867097637455	25.630455985362037	28.75101764454583	21.61065927245468
36	23.019482351423896	25.15149805337697	24.582261951781785	27.246757643417354
37	22.755902338365804	26.736363563085902	27.94447892568978	22.56325517285851
38	22.279362571638146	24.15730971054562	27.754088714422743	25.809239003393493
39	24.491500149120192	25.16649067797293	26.660433174003114	23.681575998903764
40	22.263402680939215	24.93644255648431	30.56399674353745	22.23615801903902
41	23.19520236012929	26.20065935306019	25.64947888538703	24.95465940142349
42	22.717211694247187	26.466496320358534	28.431013775481418	22.385278209912865
43	24.428950274461755	23.74589919475097	27.987683478288904	23.83746705249837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	67.5
4	124.0
5	124.0
6	172.0
7	220.0
8	213.5
9	207.0
10	306.0
11	405.0
12	405.0
13	630.0
14	855.0
15	2013.0
16	3171.0
17	3615.5
18	4060.0
19	4060.0
20	4053.0
21	4046.0
22	5077.5
23	6109.0
24	7909.5
25	9710.0
26	9710.0
27	11444.0
28	13178.0
29	15348.0
30	17518.0
31	20188.0
32	22858.0
33	22858.0
34	29932.5
35	37007.0
36	37969.5
37	38932.0
38	38459.5
39	37987.0
40	37987.0
41	38038.0
42	38089.0
43	39651.0
44	41213.0
45	40595.0
46	39977.0
47	39977.0
48	38893.5
49	37810.0
50	39662.5
51	41515.0
52	52948.0
53	64381.0
54	64381.0
55	52616.0
56	40851.0
57	41056.0
58	41261.0
59	35244.0
60	29227.0
61	29227.0
62	23403.0
63	17579.0
64	13495.5
65	9412.0
66	7904.5
67	6397.0
68	6397.0
69	5541.5
70	4686.0
71	5223.0
72	5760.0
73	5202.0
74	4644.0
75	4644.0
76	2608.0
77	572.0
78	427.5
79	283.0
80	203.0
81	123.0
82	123.0
83	97.5
84	72.0
85	52.0
86	32.0
87	21.5
88	11.0
89	11.0
90	7.5
91	4.0
92	2.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.35236879136865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01509921206164	39.65624323562293
2	10.69083282957464	10.338541833249284
3	2.875962594705325	4.1717881202812
4	1.2199084737438928	2.359418576567214
5	0.6335304090235644	1.5316347988827004
6	0.442110726060708	1.2826260523864266
7	0.2980398334295409	1.0087652358352268
8	0.21762367006559702	0.8418095962194304
9	0.1760983437174762	0.7663294853078946
>10	1.1962595411226118	11.352713262041844
>50	0.1241258920418305	4.183613362777874
>100	0.09367991771789831	9.256655003767701
>500	0.011040847445323729	3.7731950595574597
>1k	0.004349424751188135	3.949204158545563
>5k	0.001003713404120339	3.7018531361624656
>10k+	3.345711347067796E-4	1.8256090827948008
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	11285	1.8192663286609005	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	9933	1.6013090334593465	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	7520	1.2123068490500641	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	5430	0.8753758231837563	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3899	0.6285617559103989	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2595	0.41834258953256864	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	2524	0.40689660731414384	Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2040	0.32887047500826205	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1982	0.3195202360129291	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1966	0.3169408597383545	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.31355542837797534	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1446	0.23311113081467988	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1415	0.22811358928269157	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1391	0.22424452487082966	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1173	0.18910052312975068	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	1018	0.16411281546980921	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1018	0.16411281546980921	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	991	0.15976011800646456	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	990	0.15959890698930365	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	964	0.1554074205431199	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	917	0.147830502736557	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	911	0.14686323663359155	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	875	0.14105964001579868	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	862	0.1389638967927068	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	843	0.13590088746664947	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	816	0.1315481900033048	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	808	0.1302585018660175	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	729	0.1175228315103054	No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT	729	0.1175228315103054	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	713	0.11494345523573081	No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT	702	0.11317013404696077	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.10946228065225978	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	674	0.10865622556645521	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	674	0.10865622556645521	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	671	0.10817259251497248	No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC	640	0.10317505098298418	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	629	0.10140172979421413	No Hit
GTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGG	625	0.1007568857255705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6121101716091277E-4	0.0
5	0.0	0.0	0.0	1.6121101716091277E-4	0.0
6	0.0	0.0	0.0	1.6121101716091277E-4	0.0
7	0.0	0.0	0.0	1.6121101716091277E-4	0.0
8	0.0	0.0	0.0	1.6121101716091277E-4	0.0
9	0.0	0.0	0.0	1.6121101716091277E-4	0.0
10	0.0	0.0	0.0	1.6121101716091277E-4	0.0
11	0.0	0.0	0.0	1.6121101716091277E-4	0.0
12	0.0	0.0	0.0	1.6121101716091277E-4	0.0
13	0.0	0.0	0.0	1.6121101716091277E-4	0.0
14	0.0	0.0	0.0	1.6121101716091277E-4	0.0
15	0.0	0.0	0.0	1.6121101716091277E-4	0.0
16	0.0	0.0	0.0	3.2242203432182554E-4	0.0
17	0.0	0.0	0.0	3.2242203432182554E-4	0.0
18	0.0	0.0	0.0	3.2242203432182554E-4	0.0
19	0.0	0.0	0.0	3.2242203432182554E-4	0.0
20	0.0	0.0	0.0	3.2242203432182554E-4	0.0
21	0.0	0.0	0.0	4.8363305148273834E-4	0.0
22	0.0	0.0	0.0	9.672661029654767E-4	0.0
23	0.0	0.0	0.0	0.001450899154448215	0.0
24	0.0	0.0	0.0	0.0017733211887700406	0.0
25	0.0	0.0	0.0	0.002095743223091866	0.0
26	0.0	0.0	0.0	0.002256954240252779	0.0
27	0.0	0.0	0.0	0.0025793762745746044	0.0
28	0.0	0.0	0.0	0.0074157067894019876	0.0
29	0.0	0.0	0.0	0.02095743223091866	0.0
30	0.0	0.0	0.0	0.032564625466504384	0.0
31	0.0	0.0	0.0	0.07238374670524984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	75	0.0	34.533333	1
CTTATAC	1285	0.0	32.968872	1
CGTCTTC	370	0.0	31.5	37
TCGAGTA	25	0.0054944176	29.599998	2
TATACAC	1470	0.0	29.323128	3
TTATACA	1495	0.0	28.585287	2
TGCGTTA	35	8.8647753E-4	26.42857	37
TTACGGG	100	0.0	25.899998	3
GTATCAA	1070	0.0	25.761683	1
ATTACGG	105	1.8189894E-12	24.666666	2
CCGTCTT	1025	0.0	24.185366	37
GCCGTCT	1035	0.0	23.951689	36
GGTATCA	310	0.0	23.870966	1
AACCACG	95	1.6730155E-7	19.473684	35
TGTGCTA	95	1.6730155E-7	19.473684	26
GTGCTAG	105	2.2544555E-8	19.38095	27
ATAGGAC	70	1.2180497E-4	18.5	3
GCGAACT	60	9.2302816E-4	18.5	28
CTATTAA	130	6.9485395E-10	18.5	1
CCGTGGC	50	0.0070313215	18.499998	9
>>END_MODULE