FastQCFastQC Report
Fri 10 Feb 2017
ERR1630892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114999
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT77706.756580492004279RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG68935.993965164914478No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT59685.189610344437778No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC40923.558291811233141No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC25862.2487152062191846No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC25682.2330628961990975RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11961.0400090435569005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10240.890442525587179No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG9810.8530508960947486No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6610.5747876068487552No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5940.5165262306628753No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5600.4869607561804885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4100.3565248393464291No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3620.3147853459595301No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT2620.22782806807015712No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2320.20174088470334525No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1970.17130583744206473No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1770.15391438186419013No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1610.14000121740189045No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA1610.14000121740189045No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1480.12869677127627197No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1450.12608805293959077No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1450.12608805293959077No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA1430.12434890738180332No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1430.12434890738180332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.1147836068139723No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAA1290.11217488847729111No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG1250.10869659736171618No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1250.10869659736171618No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1180.10260958790946008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACAC308.247032E-637.018
GATTACG308.247032E-637.01
TTACGGG308.247032E-637.03
CGTCTTC2950.035.7457637
CTTATAC6700.035.0671651
TTATACA7450.031.5369112
GGTATCA1300.029.8846151
CAACCCA250.005476042629.617
CAATTAT250.005476042629.61
TTTACAC250.005476042629.62
TAAAGCC250.005476042629.614
CATAAGG250.005476042629.637
TATACAC8100.029.4629633
ACAGACA405.8944526E-527.7517
AGACAGA405.8944526E-527.7515
CCGTCTT11150.026.71300337
TATATTA358.8207214E-426.4285725
TTACACA358.8207214E-426.4285723
GCGCAAG358.8207214E-426.42857222
GCCGTCT11400.026.12719236
Produced by FastQC (version 0.11.5)