##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630892.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114999
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224906303533075	31.0	31.0	34.0	28.0	34.0
2	31.316559274428474	31.0	31.0	34.0	28.0	34.0
3	30.79920694962565	31.0	31.0	34.0	27.0	34.0
4	34.888581639840346	37.0	35.0	37.0	32.0	37.0
5	35.07323541943843	35.0	35.0	37.0	32.0	37.0
6	35.27828068070157	37.0	35.0	37.0	32.0	37.0
7	35.228236767276236	37.0	35.0	37.0	32.0	37.0
8	35.29732432455934	37.0	35.0	37.0	32.0	37.0
9	37.077139801215665	39.0	37.0	39.0	33.0	39.0
10	36.93606031356794	39.0	37.0	39.0	33.0	39.0
11	36.91878190245133	39.0	37.0	39.0	33.0	39.0
12	36.81662449238689	39.0	37.0	39.0	32.0	39.0
13	36.93796467795372	39.0	37.0	39.0	33.0	39.0
14	37.789476430229826	39.0	37.0	41.0	33.0	41.0
15	37.79721562796198	39.0	37.0	41.0	33.0	41.0
16	37.860190088609464	39.0	37.0	41.0	33.0	41.0
17	37.86033791598188	39.0	37.0	41.0	33.0	41.0
18	37.99293906903538	40.0	37.0	41.0	33.0	41.0
19	37.96086922494978	40.0	37.0	41.0	33.0	41.0
20	37.89323385420743	40.0	37.0	41.0	33.0	41.0
21	37.868546683014635	39.0	37.0	41.0	33.0	41.0
22	37.686971191053836	39.0	37.0	41.0	32.0	41.0
23	37.71774537169888	39.0	37.0	41.0	32.0	41.0
24	37.64530995921704	39.0	37.0	41.0	32.0	41.0
25	37.58219636692493	39.0	37.0	41.0	32.0	41.0
26	37.35240306437447	39.0	36.0	41.0	31.0	41.0
27	37.24529778519813	39.0	36.0	40.0	31.0	41.0
28	37.2571935408134	39.0	36.0	40.0	31.0	41.0
29	37.22539326428925	39.0	36.0	40.0	31.0	41.0
30	37.03123505421786	39.0	36.0	40.0	31.0	41.0
31	36.848642162105754	39.0	35.0	40.0	31.0	41.0
32	36.63304028730685	39.0	35.0	40.0	30.0	41.0
33	36.274524126296754	38.0	35.0	40.0	30.0	41.0
34	36.22020191479926	38.0	35.0	40.0	30.0	41.0
35	36.30280263306638	38.0	35.0	40.0	30.0	41.0
36	36.16894929521126	38.0	35.0	40.0	29.0	41.0
37	36.107844416038404	38.0	35.0	40.0	28.0	41.0
38	36.08293115592309	38.0	35.0	40.0	28.0	41.0
39	35.7400586092053	38.0	34.0	40.0	27.0	41.0
40	35.323115853181335	38.0	34.0	40.0	26.0	41.0
41	35.34412473151941	38.0	34.0	40.0	26.0	41.0
42	35.22448021287141	38.0	34.0	40.0	26.0	41.0
43	34.242028191549494	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	6.0
11	7.0
12	10.0
13	6.0
14	13.0
15	6.0
16	1.0
17	6.0
18	6.0
19	18.0
20	21.0
21	49.0
22	110.0
23	167.0
24	347.0
25	479.0
26	793.0
27	1120.0
28	1603.0
29	2137.0
30	2723.0
31	3457.0
32	4383.0
33	5445.0
34	6983.0
35	9160.0
36	12365.0
37	17865.0
38	25779.0
39	19927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.48807380933747	25.36022052365673	19.88191201662623	26.26979365037957
2	10.752267411020966	20.24974130209828	42.494282558978774	26.50370872790198
3	11.60184001600014	31.84723345420395	37.80206784406821	18.7488586857277
4	8.296593883425073	19.955825702832197	28.72111931408099	43.02646109966173
5	11.41575144131688	38.050765658831814	32.03158288332942	18.501900016521883
6	25.753267419716693	36.858581378968516	16.111444447343022	21.276706753971773
7	20.028000243480378	36.04292211236619	17.413194897346933	26.515882746806497
8	27.970678005895707	29.048948251723928	24.449777824154992	18.530595918225377
9	18.46885625092392	12.837502934808128	14.461864885781617	54.231775928486336
10	14.754910912268802	25.382829415907963	29.337646414316648	30.524613257506587
11	27.327194149514344	20.066261445751703	22.580196349533473	30.02634805520048
12	16.461882277237194	30.50113479247646	20.343655162218802	32.69332776806755
13	33.62811850537831	15.294046035182914	23.28541987321629	27.79241558622249
14	23.718467117105366	25.621092357324848	20.516700145218657	30.143740380351137
15	26.86805972225845	28.144592561674447	17.023626292402543	27.963721423664555
16	30.653310028782858	22.01758276158923	17.779285037261193	29.549822172366717
17	17.221888885990314	37.309889651214355	18.236680318959294	27.231541143836036
18	24.73847598674771	17.95319959303994	22.473238897729544	34.83508552248281
19	21.784537256845713	28.619379298950427	28.42459499647823	21.171488447725633
20	18.06450490873834	30.73852816111444	20.749745649962172	30.447221280185044
21	28.631553317854934	26.4454473517161	23.065417960156175	21.857581370272786
22	25.64283167679719	36.0420525395873	21.089748606509623	17.225367177105888
23	30.375046739536867	20.79148514334907	21.768884946825626	27.064583170288437
24	25.42109061817929	26.593274724128037	24.409777476325882	23.575857181366793
25	26.83501595666049	31.32027235019435	20.75844137775111	21.086270315394046
26	22.478456334402907	32.382890286002485	27.16110574874564	17.977547630848964
27	22.68367551022183	37.11684449429995	23.504552213497508	16.694927781980713
28	19.0401655666571	27.902851329141992	29.434168992773852	23.622814111427058
29	23.327159366603187	21.697579978956337	28.951556100487828	26.02370455395264
30	26.40718614944478	25.564570126696758	30.119392342542113	17.90885138131636
31	20.868877120670614	27.32197671284098	20.553222201932194	31.255923964556214
32	34.97073887599023	21.57670936269011	26.175879790259042	17.276671971060615
33	22.000191306011356	26.418490595570397	32.05767006669623	19.523648031722015
34	18.271463230115046	31.082878981556362	24.374124992391238	26.271532795937357
35	23.85672918894947	19.27755893529509	40.639483821598446	16.226228054156994
36	24.224558474421517	26.79501560883138	20.925399351298708	28.055026565448394
37	19.561909233993337	23.825424568909295	30.86548578683293	25.747180410264438
38	20.355829181123315	18.595813876642406	30.207219193210378	30.841137749023908
39	29.37329889825129	20.802789589474692	25.44022121931495	24.38369029295907
40	16.74275428481987	17.688849468256247	43.64994478212854	21.918451464795346
41	23.17672327585457	23.088896425186306	23.266289272080627	30.468091026878497
42	20.99844346472578	18.627988069461473	39.14990565135349	21.223662814459257
43	23.219332342020365	17.777545891703404	34.40464699693041	24.59847476934582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	6.0
2	4.0
3	23.5
4	43.0
5	43.0
6	63.5
7	84.0
8	85.0
9	86.0
10	141.0
11	196.0
12	196.0
13	291.0
14	386.0
15	1053.5
16	1721.0
17	1654.5
18	1588.0
19	1588.0
20	1297.5
21	1007.0
22	758.0
23	509.0
24	577.5
25	646.0
26	646.0
27	803.5
28	961.0
29	1117.0
30	1273.0
31	1547.5
32	1822.0
33	1822.0
34	2259.0
35	2696.0
36	3104.5
37	3513.0
38	4155.5
39	4798.0
40	4798.0
41	5114.5
42	5431.0
43	5861.5
44	6292.0
45	6777.0
46	7262.0
47	7262.0
48	8595.5
49	9929.0
50	15657.5
51	21386.0
52	14577.5
53	7769.0
54	7769.0
55	13416.5
56	19064.0
57	14980.0
58	10896.0
59	6735.5
60	2575.0
61	2575.0
62	2039.0
63	1503.0
64	1140.5
65	778.0
66	611.5
67	445.0
68	445.0
69	317.5
70	190.0
71	143.5
72	97.0
73	59.0
74	21.0
75	21.0
76	17.5
77	14.0
78	8.0
79	2.0
80	1.0
81	0.0
82	0.0
83	0.5
84	1.0
85	1.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	114999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.559352690023392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.36321573163679	17.4731954190906
2	11.679651617732114	5.970486699884347
3	5.552342394447658	4.2574283254636995
4	3.28989895553363	3.3635075087609456
5	2.085530568502705	2.665240567309281
6	1.6194331983805668	2.4834998565204915
7	1.0614772224679345	1.8991469491039052
8	0.9560099343381078	1.954799606953104
9	0.6634232640424591	1.5261002269584953
>10	4.395604395604396	20.967138844685607
>50	0.21433674684448678	3.739162949243037
>100	0.07824992345116184	3.3582900720875837
>500	0.013608682339332493	2.4313254897868677
>1k	0.01701085292416562	9.970521482795503
>5k	0.01020651175449937	17.940156001356534
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7770	6.756580492004279	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	6893	5.993965164914478	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	5968	5.189610344437778	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	4092	3.558291811233141	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2586	2.2487152062191846	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2568	2.2330628961990975	RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1196	1.0400090435569005	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.890442525587179	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	981	0.8530508960947486	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	661	0.5747876068487552	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	594	0.5165262306628753	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	560	0.4869607561804885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	410	0.3565248393464291	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	362	0.3147853459595301	No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	262	0.22782806807015712	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	232	0.20174088470334525	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	197	0.17130583744206473	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	177	0.15391438186419013	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	161	0.14000121740189045	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	161	0.14000121740189045	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	148	0.12869677127627197	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	145	0.12608805293959077	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	145	0.12608805293959077	No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA	143	0.12434890738180332	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	143	0.12434890738180332	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.1147836068139723	No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAA	129	0.11217488847729111	No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG	125	0.10869659736171618	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	125	0.10869659736171618	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	118	0.10260958790946008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	8.695727788937296E-4	0.0
21	0.0	0.0	0.0	8.695727788937296E-4	0.0
22	0.0	0.0	0.0	0.0034782911155749182	0.0
23	0.0	0.0	0.0	0.005217436673362377	0.0
24	0.0	0.0	0.0	0.006087009452256107	0.0
25	0.0	0.0	0.0	0.006087009452256107	0.0
26	0.0	0.0	0.0	0.007826155010043566	0.0
27	0.0	0.0	0.0	0.007826155010043566	0.0
28	0.0	0.0	0.0	0.016521882798980862	0.0
29	0.0	0.0	0.0	0.033043765597961725	0.0
30	0.0	0.0	0.0	0.05478308507030496	0.0
31	0.0	0.0	0.0	0.09391386012052279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGACAC	30	8.247032E-6	37.0	18
GATTACG	30	8.247032E-6	37.0	1
TTACGGG	30	8.247032E-6	37.0	3
CGTCTTC	295	0.0	35.74576	37
CTTATAC	670	0.0	35.067165	1
TTATACA	745	0.0	31.536911	2
GGTATCA	130	0.0	29.884615	1
CAACCCA	25	0.0054760426	29.6	17
CAATTAT	25	0.0054760426	29.6	1
TTTACAC	25	0.0054760426	29.6	2
TAAAGCC	25	0.0054760426	29.6	14
CATAAGG	25	0.0054760426	29.6	37
TATACAC	810	0.0	29.462963	3
ACAGACA	40	5.8944526E-5	27.75	17
AGACAGA	40	5.8944526E-5	27.75	15
CCGTCTT	1115	0.0	26.713003	37
TATATTA	35	8.8207214E-4	26.428572	5
TTACACA	35	8.8207214E-4	26.428572	3
GCGCAAG	35	8.8207214E-4	26.428572	22
GCCGTCT	1140	0.0	26.127192	36
>>END_MODULE