FastQCFastQC Report
Fri 10 Feb 2017
ERR1630891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742023
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT154162.077563633472278No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG105831.4262361139749038No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT101371.366130160385864RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC52270.7044256040580952No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC43220.5824617296229362RNA PCR Primer, Index 27 (95% over 24bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG33980.4579372876581993No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC28260.3808507283466954RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25250.3402859480096978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.3258659098168116No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG20040.2700724910144295No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA16970.22869911040493354No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13360.18004832734295298No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC12640.1703451240729735No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG12400.16711072298298033No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11030.14864768342760265No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA10890.14676094945843995No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT10420.1404269139905367No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC10310.13894448015762315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9820.13234091126555378No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT9290.1251982755251522No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG9260.12479397538890304No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8970.12088574073849463No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC8730.11765133964850147No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA8690.11711227280016927No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT8350.11253020458934561No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC8130.10956533692351854No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT8040.10835243651477111No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG7980.1075438362422728No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT7630.10282700131936612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18700.032.943851
GATTACG750.032.0666661
TTATACA19850.031.1284642
TATACAC20650.030.7288153
CGTCTTC5850.030.67521337
TAGCCCG250.00549510329.610
CGTACAT405.9373357E-527.7512
TTACGGG1050.026.428573
AACGGCC901.4188117E-1024.66666837
TTAACGG901.4188117E-1024.66666835
ACGTACA451.3225945E-424.66666811
GTATCAA11950.024.1506291
GCCGTCT16550.024.03323436
CCGTCTT16500.023.99393837
GGTATCA4450.022.8651681
TAGACTT502.7008515E-422.25
TAACGGC1107.4578566E-1121.86363636
ATTACGG1151.3460522E-1020.9130442
ATACACA30800.020.7224034
CCGGACG450.003824548620.55555735
Produced by FastQC (version 0.11.5)