##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630891.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742023
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507590735058077	31.0	31.0	34.0	28.0	34.0
2	31.511854753828384	31.0	31.0	34.0	30.0	34.0
3	31.004782870611827	31.0	31.0	34.0	27.0	34.0
4	35.143296097290786	37.0	35.0	37.0	32.0	37.0
5	35.32841165300806	37.0	35.0	37.0	33.0	37.0
6	35.517597163430246	37.0	35.0	37.0	33.0	37.0
7	35.51793812321181	37.0	35.0	37.0	33.0	37.0
8	35.53315463267311	37.0	35.0	37.0	33.0	37.0
9	37.33105981890049	39.0	37.0	39.0	34.0	39.0
10	37.150321485991675	39.0	37.0	39.0	33.0	39.0
11	37.21014442948534	39.0	37.0	39.0	33.0	39.0
12	37.126738659044264	39.0	37.0	39.0	33.0	39.0
13	37.215664474012264	39.0	37.0	39.0	34.0	39.0
14	38.35579894423758	40.0	38.0	41.0	34.0	41.0
15	38.35255645714486	40.0	38.0	41.0	34.0	41.0
16	38.35743905512363	40.0	38.0	41.0	34.0	41.0
17	38.30601881612834	40.0	38.0	41.0	33.0	41.0
18	38.353978245957336	40.0	38.0	41.0	34.0	41.0
19	38.39147843126157	40.0	38.0	41.0	34.0	41.0
20	38.350567300474516	40.0	38.0	41.0	34.0	41.0
21	38.30618323151708	40.0	38.0	41.0	34.0	41.0
22	38.26665211186176	40.0	38.0	41.0	34.0	41.0
23	38.25174960883962	40.0	38.0	41.0	34.0	41.0
24	38.214657766673	40.0	38.0	41.0	33.0	41.0
25	38.17527489040097	40.0	38.0	41.0	33.0	41.0
26	38.07157864378867	40.0	37.0	41.0	33.0	41.0
27	37.99255413915741	40.0	37.0	41.0	33.0	41.0
28	37.967208563615955	40.0	37.0	41.0	33.0	41.0
29	37.92586348401599	40.0	37.0	41.0	33.0	41.0
30	37.82264835456583	40.0	37.0	41.0	33.0	41.0
31	37.74641486854181	40.0	37.0	41.0	33.0	41.0
32	37.70266689846541	40.0	37.0	41.0	33.0	41.0
33	37.66899274011722	40.0	37.0	41.0	33.0	41.0
34	37.60498529021338	40.0	37.0	41.0	32.0	41.0
35	37.596627058729986	40.0	37.0	41.0	32.0	41.0
36	37.55980744532177	40.0	37.0	41.0	32.0	41.0
37	37.47784233103287	40.0	37.0	41.0	32.0	41.0
38	37.45517726539474	40.0	37.0	41.0	32.0	41.0
39	37.34405402527954	39.0	36.0	41.0	31.0	41.0
40	37.241183898612306	39.0	36.0	41.0	31.0	41.0
41	37.193293199806476	39.0	36.0	41.0	31.0	41.0
42	37.12206494946922	39.0	36.0	41.0	31.0	41.0
43	36.464439781516205	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	7.0
10	9.0
11	5.0
12	4.0
13	8.0
14	7.0
15	6.0
16	9.0
17	13.0
18	17.0
19	37.0
20	62.0
21	148.0
22	313.0
23	487.0
24	962.0
25	1598.0
26	2677.0
27	4127.0
28	6139.0
29	8941.0
30	12198.0
31	15957.0
32	20744.0
33	27581.0
34	36316.0
35	49250.0
36	68529.0
37	102060.0
38	174138.0
39	209667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.29959044396198	21.96832173665776	14.761941341440899	23.970146477939362
2	13.97908151095047	22.07155303811337	41.52593652757394	22.423428923362213
3	14.509792823133516	28.20977247335999	34.12966983503207	23.150764868474425
4	11.04386252178167	19.50209090553797	37.98669313484892	31.46735343783144
5	12.67278777072948	37.58886180077976	36.16949878912109	13.568851639369669
6	28.017056075081232	40.358317734086405	17.43153514109401	14.193091049738351
7	25.716992599959838	32.44481640056979	22.582453643620212	19.255737355850155
8	24.97092408186808	35.1867799246115	21.998105180028112	17.844190813492304
9	23.66759520931292	14.640381767141989	19.706262474343784	41.98576054920131
10	14.60412952159165	28.931852516700964	33.75070584065453	22.71331212105285
11	31.225581956354453	23.58633088192684	25.159732245496436	20.028354916222273
12	20.5449157236366	26.063747350149523	29.41728221362411	23.97405471258977
13	28.811640609522886	20.748548225594085	27.156301085006803	23.28351007987623
14	21.87317643792713	22.308068617819124	26.362929450973894	29.455825493279857
15	23.477035078427487	29.170659130512128	23.340381632375276	24.011924158685108
16	25.48478955504075	27.348882716573474	23.398600851995155	23.767726876390626
17	21.801884847235193	30.180735637574575	24.651122674095006	23.366256841095222
18	24.03146533193715	25.00798492769092	26.563462318553466	24.397087421818462
19	25.902835895922365	26.751596648621405	25.784375956001366	21.561191499454868
20	24.059901108186672	26.59324576192382	25.55379011162727	23.793063018262238
21	23.877292213314142	27.142554880374327	25.108925195041127	23.871227711270404
22	22.366557370863166	28.809619108841638	26.40484189843172	22.418981621863473
23	22.46345463685088	25.44692010894541	27.861939589473643	24.22768566473007
24	23.514904524522827	27.782966296192974	25.41147646366757	23.290652715616634
25	21.988401976758134	28.08309176400192	26.983799693540494	22.944706565699448
26	25.848390144240813	26.536239442712695	26.388804659693836	21.22656575335266
27	22.64875886596507	28.66191479239862	25.577104752817636	23.112221588818677
28	21.295701076651262	26.436512075771233	28.721481679139327	23.546305168438174
29	24.118389861230717	26.13207407317563	26.217920468772533	23.531615596821123
30	23.552504437193996	25.644622875571244	28.52499181292224	22.277880874312523
31	22.228825791114293	26.451875480948704	26.599984097527972	24.71931463040903
32	21.83665465895262	26.0114578658613	26.990403262432565	25.161484212753514
33	21.120639117655383	25.100973959028224	31.931624760957543	21.84676216235885
34	22.00780838329809	27.336079878925585	26.783401592673002	23.87271014510332
35	21.871694004094213	25.668611350322024	29.23103461752533	23.228660028058428
36	22.87328020829543	25.392070057127608	27.118970705759793	24.615679028817166
37	22.113735018995367	27.044444713977867	28.078240162366935	22.763580104659827
38	22.004034915359767	24.649775006974178	28.19306140106169	25.153128676604364
39	23.905188922715332	25.333042237235233	27.13999431284475	23.621774527204682
40	21.707817682201224	25.062026379236222	31.30765488401303	21.922501054549524
41	22.513318320321606	25.976418520719708	26.236518275039995	25.273744883918692
42	22.623557490805542	26.336919475541865	29.129285749902635	21.910237283749964
43	23.766379209269793	23.984296982708084	28.969587196084216	23.279736611937903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	11.5
2	19.0
3	93.5
4	168.0
5	168.0
6	233.5
7	299.0
8	288.5
9	278.0
10	419.0
11	560.0
12	560.0
13	850.0
14	1140.0
15	2606.0
16	4072.0
17	4670.0
18	5268.0
19	5268.0
20	5427.0
21	5586.0
22	7022.0
23	8458.0
24	10540.5
25	12623.0
26	12623.0
27	14877.0
28	17131.0
29	19972.5
30	22814.0
31	26131.0
32	29448.0
33	29448.0
34	36515.0
35	43582.0
36	44495.5
37	45409.0
38	46114.5
39	46820.0
40	46820.0
41	47063.0
42	47306.0
43	48413.5
44	49521.0
45	49689.5
46	49858.0
47	49858.0
48	48618.5
49	47379.0
50	48484.5
51	49590.0
52	62179.0
53	74768.0
54	74768.0
55	60628.5
56	46489.0
57	47082.5
58	47676.0
59	40780.0
60	33884.0
61	33884.0
62	26474.5
63	19065.0
64	14605.5
65	10146.0
66	8499.0
67	6852.0
68	6852.0
69	5763.0
70	4674.0
71	5129.5
72	5585.0
73	4988.0
74	4391.0
75	4391.0
76	2507.0
77	623.0
78	437.5
79	252.0
80	207.0
81	162.0
82	162.0
83	114.0
84	66.0
85	50.0
86	34.0
87	23.5
88	13.0
89	13.0
90	11.5
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	742023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.710823798382904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.33835025480427	37.52670523004933
2	11.745542327100745	10.972879161153024
3	3.168279999644246	4.439789064219691
4	1.2858029160359026	2.402436538015999
5	0.6969984609041145	1.6278686147518084
6	0.45717703303084006	1.2813069500742629
7	0.294729426392569	0.9636938013095229
8	0.24970982838334882	0.9331321435471231
9	0.19041204549679563	0.8004873155661442
>10	1.2977881148617585	12.173359538581368
>50	0.15398979011476338	4.98090819189922
>100	0.10700982629693108	9.699561554295762
>500	0.008989985407058275	2.897378946494887
>1k	0.0040599934096392214	3.6974126113692996
>5k	2.8999952925994434E-4	0.7080555310359893
>10k+	8.699985877798331E-4	4.8950248076366
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	15416	2.077563633472278	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	10583	1.4262361139749038	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	10137	1.366130160385864	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	5227	0.7044256040580952	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	4322	0.5824617296229362	RNA PCR Primer, Index 27 (95% over 24bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3398	0.4579372876581993	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	2826	0.3808507283466954	RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2525	0.3402859480096978	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2418	0.3258659098168116	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	2004	0.2700724910144295	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1697	0.22869911040493354	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1336	0.18004832734295298	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1264	0.1703451240729735	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1240	0.16711072298298033	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1103	0.14864768342760265	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1089	0.14676094945843995	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1042	0.1404269139905367	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1031	0.13894448015762315	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	982	0.13234091126555378	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	929	0.1251982755251522	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	926	0.12479397538890304	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	897	0.12088574073849463	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	873	0.11765133964850147	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	869	0.11711227280016927	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	835	0.11253020458934561	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	813	0.10956533692351854	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	804	0.10835243651477111	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	798	0.1075438362422728	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	763	0.10282700131936612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.695334241660973E-4	0.0
9	0.0	0.0	0.0	4.0430013624914593E-4	0.0
10	0.0	0.0	0.0	6.738335604152432E-4	0.0
11	0.0	0.0	0.0	8.086002724982919E-4	0.0
12	0.0	0.0	0.0	8.086002724982919E-4	0.0
13	0.0	0.0	0.0	8.086002724982919E-4	0.0
14	0.0	0.0	0.0	8.086002724982919E-4	0.0
15	0.0	0.0	0.0	9.433669845813405E-4	0.0
16	0.0	0.0	0.0	9.433669845813405E-4	0.0
17	0.0	0.0	0.0	9.433669845813405E-4	0.0
18	0.0	0.0	0.0	0.0012129004087474379	0.0
19	0.0	0.0	0.0	0.0012129004087474379	0.0
20	0.0	0.0	0.0	0.0013476671208304863	0.0
21	0.0	0.0	0.0	0.0013476671208304863	0.0
22	0.0	0.0	0.0	0.0017519672570796322	0.0
23	0.0	0.0	0.0	0.0020215006812457294	0.0
24	0.0	0.0	0.0	0.0026953342416609727	0.0
25	0.0	0.0	0.0	0.0030996343779101185	0.0
26	0.0	0.0	0.0	0.0032344010899931675	0.0
27	0.0	0.0	0.0	0.004312534786657557	0.0
28	0.0	0.0	0.0	0.01091610367872694	0.0
29	0.0	0.0	0.0	0.024258008174948754	0.0
30	0.0	0.0	0.0	0.041373380609495934	0.0
31	0.0	0.0	0.0	0.08625069573315113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1870	0.0	32.94385	1
GATTACG	75	0.0	32.066666	1
TTATACA	1985	0.0	31.128464	2
TATACAC	2065	0.0	30.728815	3
CGTCTTC	585	0.0	30.675213	37
TAGCCCG	25	0.005495103	29.6	10
CGTACAT	40	5.9373357E-5	27.75	12
TTACGGG	105	0.0	26.42857	3
AACGGCC	90	1.4188117E-10	24.666668	37
TTAACGG	90	1.4188117E-10	24.666668	35
ACGTACA	45	1.3225945E-4	24.666668	11
GTATCAA	1195	0.0	24.150629	1
GCCGTCT	1655	0.0	24.033234	36
CCGTCTT	1650	0.0	23.993938	37
GGTATCA	445	0.0	22.865168	1
TAGACTT	50	2.7008515E-4	22.2	5
TAACGGC	110	7.4578566E-11	21.863636	36
ATTACGG	115	1.3460522E-10	20.913044	2
ATACACA	3080	0.0	20.722403	4
CCGGACG	45	0.0038245486	20.555557	35
>>END_MODULE