FastQCFastQC Report
Fri 10 Feb 2017
ERR1630890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859583
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT158561.8446153541891823No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG126701.473970518262925No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT95431.1101894755945616Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC68230.7937569728577695No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA65910.7667671417419841No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC36640.42625319486309055Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35940.4181097113367761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.38518677079467606No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC32610.37936999684730854No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC22390.2604751373631168No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA19650.22859921613154285No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG16940.19707230133681097No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16090.18718378562628624No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15700.1826467019473396No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA15200.17682992799997208No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG14790.17206017336313073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12350.1436743164999773No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT12250.14251096171050381No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA12130.14111493596313562No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC11690.1359961748894522No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA10790.12552598178419072No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC10620.12354827864208574No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG10250.11924386592103381No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT9480.11028603404208785No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9210.1071449761105094No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT8960.10423658913682565No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA8850.10295689886840481No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG8630.1003975183315631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20250.033.711111
GCGTTAT401.5994465E-632.3751
TTATACA21550.031.5916482
TATACAC22200.031.0833343
ATAACGC303.5987308E-430.8333323
CGTCTTC5300.030.36792637
ATCGACT250.005495582729.59999818
GATTACG803.274181E-1127.751
GGTATCA5650.027.5044251
TTACGGG856.91216E-1126.1176473
CCGTCTT16400.025.49390437
GCTTTAT12300.024.967481
GCCGTCT17150.024.3790136
TTTATTG13250.024.0150953
TTATTGA13600.023.9411774
CTTTATT13200.023.4053022
ATTGAGC13300.023.368426
ACGCTAC400.00193079323.12530
CCGACAC400.00193079323.12520
GTATCAA15950.022.8495311
Produced by FastQC (version 0.11.5)