FastQCFastQC Report
Fri 10 Feb 2017
ERR1630889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500511
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT65111.3008705103384341No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG46950.938041321769152No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT39340.7859967113609891RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC23140.4623275012936779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14070.2811127028177203No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC12700.2537406770280773RNA PCR Primer, Index 26 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.23855619556812938No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC11180.22337171410818144No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5710.11408340675829302No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG5550.11088667381935662No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA5170.10329443308938265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5110.1020956582372815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC1650.031.3939437
CTTATAC8300.030.9819281
TATACAC9600.027.5572933
TTATACA9700.026.7010332
CCGTCTT6000.026.20833437
GCCGTCT6100.025.77868836
GTATCAA7750.024.8258061
GCTAAGC400.00192967223.1250028
CCCCGTA502.69915E-422.19999915
GGTATCA2350.022.0425551
TCCGTTG603.7208774E-521.58333422
TGCCGTC7350.021.39455835
CGGGATA450.003822836320.55555517
TTAACGG450.003822836320.55555535
TAGGACG450.003822836320.5555554
TGCGTTA450.003822836320.55555537
AGCAACG555.13752E-420.1818182
GCAACGC555.13752E-420.1818183
TTCCGTT656.892183E-519.92307721
ATGCCGT7950.019.77987534
Produced by FastQC (version 0.11.5)