##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630889.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500511
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.511497249810695	31.0	31.0	34.0	28.0	34.0
2	31.523149341373117	31.0	31.0	34.0	30.0	34.0
3	31.00754428973589	31.0	31.0	34.0	27.0	34.0
4	35.150394297028434	37.0	35.0	37.0	32.0	37.0
5	35.32937937427948	37.0	35.0	37.0	33.0	37.0
6	35.512851865393564	37.0	35.0	37.0	33.0	37.0
7	35.51967489226011	37.0	35.0	37.0	33.0	37.0
8	35.545234770064994	37.0	35.0	37.0	33.0	37.0
9	37.313825270573474	39.0	37.0	39.0	34.0	39.0
10	37.1522064450132	39.0	37.0	39.0	33.0	39.0
11	37.20314638439515	39.0	37.0	39.0	33.0	39.0
12	37.14795479020441	39.0	37.0	39.0	33.0	39.0
13	37.21873245543055	39.0	37.0	39.0	34.0	39.0
14	38.38512440286028	40.0	38.0	41.0	34.0	41.0
15	38.38764782392395	40.0	38.0	41.0	34.0	41.0
16	38.38335820791151	40.0	38.0	41.0	34.0	41.0
17	38.35033196073613	40.0	38.0	41.0	34.0	41.0
18	38.37800168228071	40.0	38.0	41.0	34.0	41.0
19	38.428486087218864	40.0	38.0	41.0	34.0	41.0
20	38.42743116534901	40.0	38.0	41.0	34.0	41.0
21	38.382926648964755	40.0	38.0	41.0	34.0	41.0
22	38.32679601447321	40.0	38.0	41.0	34.0	41.0
23	38.310378792873685	40.0	38.0	41.0	34.0	41.0
24	38.268647442313956	40.0	38.0	41.0	34.0	41.0
25	38.252967467248475	40.0	38.0	41.0	34.0	41.0
26	38.15835616000447	40.0	38.0	41.0	33.0	41.0
27	38.108097524330134	40.0	38.0	41.0	33.0	41.0
28	38.08770836205398	40.0	38.0	41.0	33.0	41.0
29	38.02694446275906	40.0	37.0	41.0	33.0	41.0
30	37.96445432767712	40.0	37.0	41.0	33.0	41.0
31	37.92927028576795	40.0	37.0	41.0	33.0	41.0
32	37.87191690092725	40.0	37.0	41.0	33.0	41.0
33	37.82281907890136	40.0	37.0	41.0	33.0	41.0
34	37.76424494166962	40.0	37.0	41.0	33.0	41.0
35	37.73802373973799	40.0	37.0	41.0	33.0	41.0
36	37.711358991111084	40.0	37.0	41.0	33.0	41.0
37	37.66572562840777	40.0	37.0	41.0	33.0	41.0
38	37.6357622509795	40.0	37.0	41.0	33.0	41.0
39	37.570295158348166	40.0	37.0	41.0	32.0	41.0
40	37.51396472804794	40.0	37.0	41.0	32.0	41.0
41	37.460835026602815	40.0	37.0	41.0	32.0	41.0
42	37.420895844446974	40.0	37.0	41.0	32.0	41.0
43	36.80395635660355	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	4.0
11	8.0
12	4.0
13	5.0
14	6.0
15	8.0
16	7.0
17	7.0
18	10.0
19	11.0
20	43.0
21	70.0
22	148.0
23	299.0
24	507.0
25	947.0
26	1542.0
27	2367.0
28	3731.0
29	5386.0
30	7624.0
31	10383.0
32	13947.0
33	17980.0
34	23807.0
35	32285.0
36	45246.0
37	68177.0
38	121038.0
39	144907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71723098992829	22.40390321091844	15.115951497569485	23.76291430158378
2	13.947345812579542	22.206504951939117	41.31297813634466	22.53317109913668
3	14.587091991984192	28.714054236570224	34.318326670143115	22.38052710130247
4	11.60993464679098	19.09648339397136	39.979940500808176	29.313641458429483
5	11.75558579132127	38.97856390768634	36.790600006793056	12.475250294199327
6	27.143659180317712	41.34054995794298	18.09490700504085	13.420883856698454
7	24.196071614809664	33.47978366109835	24.21784935795617	18.10629536613581
8	24.160707756672682	36.61957479455996	21.846073313074037	17.373644135693322
9	24.042029046314667	15.706747703846668	20.394556762988223	39.856666486850436
10	13.729368585305817	29.55978989472759	35.070557889836586	21.640283630130007
11	30.927791796783687	23.961311539606523	26.227795193312435	18.883101470297355
12	20.422727972012602	26.68133167902404	30.556571184249698	22.339369164713663
13	27.363634365678273	21.56176387731738	28.052730109827756	23.021871647176585
14	21.067868638251706	22.29461490356855	27.751238234524315	28.886278223655427
15	22.906789261374875	30.00992985169157	24.07399637570403	23.009284511229524
16	23.51057219521649	28.60996062024611	24.792661899538672	23.086805284998732
17	21.121613710787575	30.455474505055836	26.107717912293637	22.315193871862956
18	22.45585012117616	27.20959179718328	27.477917568245253	22.85664051339531
19	22.92856700452138	27.602190561246402	27.9782062731888	21.491036161043414
20	22.253656762788427	27.050154741853827	26.914892979375075	23.781295515982663
21	22.67822285624092	27.04815678376699	26.890717686524372	23.382902673467715
22	21.24608649959741	29.48146993772365	27.995588508544266	21.276855054134675
23	22.673227961023834	26.918888895548747	27.26093932001494	23.14694382341247
24	22.443662576846464	29.057703027505887	27.328470303349977	21.17016409229767
25	22.55634741294397	27.898487745524076	27.09570818623367	22.449456655298285
26	22.76433484978352	27.228971990625585	27.8569302173179	22.149762942272996
27	21.32660421049687	28.825740093624315	27.266333806849403	22.58132188902941
28	21.27665525832599	26.595419481290122	28.530841480007435	23.597083780376455
29	21.40352559684003	27.441954322682214	29.718028175204942	21.43649190527281
30	22.622280029809534	26.821987928337236	27.622969325349494	22.93276271650373
31	21.505621255077312	27.438757589743286	27.278721146987778	23.776900008191628
32	22.4662395032277	27.37642129743402	29.022339169368905	21.135000029969373
33	22.34716120125232	27.13546755216169	28.363013000713273	22.154358245872718
34	21.65686668225074	29.62732087806262	27.271928089492537	21.4438843501941
35	23.007086757334005	26.80380650974704	29.346607766862263	20.84249896605669
36	22.551552313535566	27.14285999708298	26.10851709552837	24.19707059385308
37	21.718803382942635	27.486109196401276	28.430743779856986	22.364343640799103
38	22.16254987402874	26.549266649484228	28.539232903972138	22.74895057251489
39	22.66523612867649	27.059744940670633	27.631760340931567	22.643258589721306
40	21.480247187374506	25.997031034282962	30.727995988100158	21.79472579024237
41	21.50721962154678	27.155846724647407	27.537456719232946	23.799476934572866
42	22.135577439856466	26.855753420004756	28.996765305857412	22.011903834281366
43	22.20390760642623	25.936692700060537	29.632915160705757	22.22648453280747
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	12.0
2	15.0
3	62.5
4	110.0
5	110.0
6	140.5
7	171.0
8	177.0
9	183.0
10	250.5
11	318.0
12	318.0
13	484.0
14	650.0
15	1515.5
16	2381.0
17	2887.0
18	3393.0
19	3393.0
20	3803.0
21	4213.0
22	5591.5
23	6970.0
24	8866.5
25	10763.0
26	10763.0
27	12963.5
28	15164.0
29	18014.0
30	20864.0
31	22920.0
32	24976.0
33	24976.0
34	27832.5
35	30689.0
36	32473.5
37	34258.0
38	35601.0
39	36944.0
40	36944.0
41	37786.5
42	38629.0
43	37678.5
44	36728.0
45	35892.5
46	35057.0
47	35057.0
48	33179.0
49	31301.0
50	31215.5
51	31130.0
52	35334.5
53	39539.0
54	39539.0
55	31916.0
56	24293.0
57	24298.5
58	24304.0
59	20365.5
60	16427.0
61	16427.0
62	13645.5
63	10864.0
64	8735.0
65	6606.0
66	5371.0
67	4136.0
68	4136.0
69	3429.5
70	2723.0
71	2526.0
72	2329.0
73	2333.5
74	2338.0
75	2338.0
76	1823.5
77	1309.0
78	914.5
79	520.0
80	318.0
81	116.0
82	116.0
83	82.0
84	48.0
85	38.5
86	29.0
87	18.5
88	8.0
89	8.0
90	6.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	500511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.608635103548444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.21593953230308	49.66782600626046
2	7.93535787998845	9.14290273048645
3	1.9305832446201008	3.3365479702903205
4	0.8798840329832646	2.0275567275828594
5	0.5199848325289999	1.497780823827145
6	0.3486420828157631	1.205087671840465
7	0.2735732941731397	1.1032128854667285
8	0.21445632307781257	0.9883628849470719
9	0.17007109996422926	0.8817807545548555
>10	1.31945513940399	15.356948005095838
>50	0.13856955289772863	5.499931056330578
>100	0.04931547989976833	4.370934654057189
>500	0.001389168447880798	0.4309513088679995
>1k	0.002431044783791397	3.187519151757181
>5k	3.472921119701995E-4	1.3026573686348861
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	6511	1.3008705103384341	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	4695	0.938041321769152	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	3934	0.7859967113609891	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	2314	0.4623275012936779	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1407	0.2811127028177203	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	1270	0.2537406770280773	RNA PCR Primer, Index 26 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.23855619556812938	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	1118	0.22337171410818144	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	571	0.11408340675829302	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	555	0.11088667381935662	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	517	0.10329443308938265	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.1020956582372815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9979580868352542E-4	0.0
5	0.0	0.0	0.0	1.9979580868352542E-4	0.0
6	0.0	0.0	0.0	1.9979580868352542E-4	0.0
7	0.0	0.0	0.0	1.9979580868352542E-4	0.0
8	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
9	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
10	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
11	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
12	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
13	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
14	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
15	1.9979580868352542E-4	0.0	0.0	1.9979580868352542E-4	0.0
16	1.9979580868352542E-4	0.0	0.0	3.9959161736705085E-4	1.9979580868352542E-4
17	1.9979580868352542E-4	0.0	0.0	5.993874260505763E-4	1.9979580868352542E-4
18	1.9979580868352542E-4	0.0	0.0	5.993874260505763E-4	1.9979580868352542E-4
19	1.9979580868352542E-4	0.0	0.0	5.993874260505763E-4	1.9979580868352542E-4
20	1.9979580868352542E-4	0.0	0.0	5.993874260505763E-4	1.9979580868352542E-4
21	1.9979580868352542E-4	0.0	0.0	5.993874260505763E-4	1.9979580868352542E-4
22	1.9979580868352542E-4	0.0	0.0	7.991832347341017E-4	1.9979580868352542E-4
23	1.9979580868352542E-4	0.0	0.0	0.001398570660784678	1.9979580868352542E-4
24	1.9979580868352542E-4	0.0	0.0	0.002397549704202305	1.9979580868352542E-4
25	1.9979580868352542E-4	0.0	0.0	0.0025973455128858306	1.9979580868352542E-4
26	1.9979580868352542E-4	0.0	0.0	0.0025973455128858306	1.9979580868352542E-4
27	1.9979580868352542E-4	0.0	0.0	0.003196732938936407	1.9979580868352542E-4
28	1.9979580868352542E-4	0.0	0.0	0.007192649112606916	1.9979580868352542E-4
29	1.9979580868352542E-4	0.0	0.0	0.012387340138378578	1.9979580868352542E-4
30	1.9979580868352542E-4	0.0	0.0	0.022976517998605426	1.9979580868352542E-4
31	1.9979580868352542E-4	0.0	0.0	0.05594282643138712	1.9979580868352542E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	165	0.0	31.39394	37
CTTATAC	830	0.0	30.981928	1
TATACAC	960	0.0	27.557293	3
TTATACA	970	0.0	26.701033	2
CCGTCTT	600	0.0	26.208334	37
GCCGTCT	610	0.0	25.778688	36
GTATCAA	775	0.0	24.825806	1
GCTAAGC	40	0.001929672	23.125002	8
CCCCGTA	50	2.69915E-4	22.199999	15
GGTATCA	235	0.0	22.042555	1
TCCGTTG	60	3.7208774E-5	21.583334	22
TGCCGTC	735	0.0	21.394558	35
CGGGATA	45	0.0038228363	20.555555	17
TTAACGG	45	0.0038228363	20.555555	35
TAGGACG	45	0.0038228363	20.555555	4
TGCGTTA	45	0.0038228363	20.555555	37
AGCAACG	55	5.13752E-4	20.181818	2
GCAACGC	55	5.13752E-4	20.181818	3
TTCCGTT	65	6.892183E-5	19.923077	21
ATGCCGT	795	0.0	19.779875	34
>>END_MODULE