FastQCFastQC Report
Fri 10 Feb 2017
ERR1630888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742228
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT141581.907500121256541No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG126461.7037891321804084No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT94231.2695559854923286TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC67570.9103671648065016No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA58810.792344131452869No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC33800.45538567663844537RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC30740.4141584526587518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30030.4045926588595418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.3795329736954144No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA17640.23766282058882174No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG16980.22877067424026043No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC15100.20344153009587349No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG11570.15588202008008323No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA11540.15547783160969406No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA11440.15413053670839688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11230.15130121741567282No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10390.13998394024477653No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT10220.13769353891257133No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC10100.13607678503101472No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT9790.13190017083699349No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG9630.129744498994918No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA8780.11829249233389202No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT8400.11317277170896274No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC7840.10562792026169857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG507.3250703E-933.3000031
CTTATAC17400.031.5775871
CGTCTTC4300.031.40697737
GGTATCA6100.030.327871
TTATACA18900.029.365082
TATACAC19700.028.829953
CCGTCTT15100.026.09602437
GCCGTCT15550.025.45980536
TTACGGG851.9736035E-923.9411753
GACGTTG400.001930545923.12500230
AGGACCG400.001930545923.1250025
GTATCAA16500.022.984851
GCTTTAT10000.022.7551
TAGGACC1005.329639E-1022.24
TTATTGA11300.020.4646024
TGCCGTC19350.020.4599535
CGGAAAT555.140734E-420.18181837
CTTTATT11700.019.9230772
TTTATTG12050.019.804983
AACCACG759.25852E-619.73333435
Produced by FastQC (version 0.11.5)