##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630888.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742228
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33697731694304	31.0	31.0	34.0	28.0	34.0
2	31.322281024159693	31.0	31.0	34.0	28.0	34.0
3	30.77051525946205	31.0	31.0	34.0	26.0	34.0
4	34.96020629779529	37.0	35.0	37.0	32.0	37.0
5	35.15414670424721	37.0	35.0	37.0	32.0	37.0
6	35.35716114185937	37.0	35.0	37.0	33.0	37.0
7	35.36655178732141	37.0	35.0	37.0	33.0	37.0
8	35.40036484745927	37.0	35.0	37.0	33.0	37.0
9	37.15596959424867	39.0	37.0	39.0	33.0	39.0
10	36.97699089767565	39.0	37.0	39.0	33.0	39.0
11	37.035048529562346	39.0	37.0	39.0	33.0	39.0
12	36.963453278507416	39.0	37.0	39.0	33.0	39.0
13	37.059578727830264	39.0	37.0	39.0	33.0	39.0
14	38.12594512737326	40.0	37.0	41.0	33.0	41.0
15	38.15274282296006	40.0	37.0	41.0	33.0	41.0
16	38.140327500444606	40.0	37.0	41.0	33.0	41.0
17	38.09796450686312	40.0	37.0	41.0	33.0	41.0
18	38.15966118227822	40.0	37.0	41.0	33.0	41.0
19	38.16733402674111	40.0	38.0	41.0	33.0	41.0
20	38.1807234434702	40.0	38.0	41.0	33.0	41.0
21	38.112243138227065	40.0	37.0	41.0	33.0	41.0
22	37.98692719757271	40.0	37.0	41.0	33.0	41.0
23	38.02506776893353	40.0	37.0	41.0	33.0	41.0
24	37.946287394169985	40.0	37.0	41.0	33.0	41.0
25	37.89995661710418	40.0	37.0	41.0	33.0	41.0
26	37.80780972962486	40.0	37.0	41.0	33.0	41.0
27	37.68441637879466	40.0	37.0	41.0	32.0	41.0
28	37.65552498693124	40.0	37.0	41.0	32.0	41.0
29	37.55771946086647	39.0	37.0	41.0	32.0	41.0
30	37.46934769370059	39.0	37.0	41.0	32.0	41.0
31	37.44241392132876	39.0	37.0	41.0	32.0	41.0
32	37.27592060660606	39.0	36.0	41.0	31.0	41.0
33	37.25937178333342	39.0	36.0	41.0	31.0	41.0
34	37.23699186772797	39.0	36.0	41.0	31.0	41.0
35	37.14716906395339	39.0	36.0	40.0	31.0	41.0
36	37.17069013834024	39.0	36.0	40.0	31.0	41.0
37	37.146353950538106	39.0	36.0	40.0	31.0	41.0
38	37.096481135176795	39.0	36.0	40.0	31.0	41.0
39	36.984386199388865	39.0	36.0	40.0	31.0	41.0
40	36.876655151786245	39.0	36.0	40.0	31.0	41.0
41	36.84402501657173	39.0	36.0	40.0	30.0	41.0
42	36.788237576593716	39.0	35.0	40.0	30.0	41.0
43	36.058337869226165	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	0.0
10	14.0
11	12.0
12	6.0
13	9.0
14	12.0
15	9.0
16	10.0
17	14.0
18	16.0
19	35.0
20	79.0
21	156.0
22	344.0
23	689.0
24	1260.0
25	2049.0
26	3288.0
27	4906.0
28	7222.0
29	10192.0
30	13723.0
31	17857.0
32	23194.0
33	30048.0
34	39712.0
35	53413.0
36	74073.0
37	111038.0
38	185068.0
39	163776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.53883173364518	22.22726709313041	15.496585954720112	23.73731521850429
2	13.954068022224977	21.76986047414002	40.04012244216063	24.235949061474372
3	15.026918952127918	28.307204794214176	34.52038996103623	22.145486292621676
4	11.478817829561807	18.857547815496048	38.310465247875314	31.35316910706683
5	12.653254795022553	37.891187074591635	35.831442629488514	13.624115500897299
6	27.706311268235634	40.2920665887032	17.042741583448752	14.958880559612412
7	24.625586746929514	32.86523817479265	22.649509315196948	19.859665763080887
8	25.590249896258292	33.34756974945704	22.78221786297472	18.27996249130995
9	24.27043981094758	14.811890685880888	19.12283018156146	41.79483932161007
10	14.784405869894426	29.148725189564395	32.74357744520552	23.323291495335667
11	31.94570940465733	23.155553280124167	24.76745690003611	20.131280415182395
12	21.60912819241527	26.457099435752895	27.47727113501512	24.45650123681672
13	30.00856879557225	20.04411043506847	25.990396481943556	23.956924287415728
14	21.75517495971588	22.220665348114057	26.8420215890535	29.18213810311656
15	24.682981509724776	29.030567426720634	22.156264651832053	24.130186411722544
16	24.69631972924762	26.775330491439288	23.71777944243548	24.810570336877618
17	22.201399031025506	30.063403698055048	23.936580134406142	23.798617136513307
18	24.577892507423595	24.646200358919362	26.438102577644607	24.337804556012436
19	26.52688931164009	26.176996825773212	25.603318656800877	21.692795205785824
20	24.571964409857888	25.119100869274668	24.478462143707862	25.83047257715958
21	26.791093841784463	24.59971868482461	25.797598581567925	22.811588891823
22	21.292379161120305	30.650554816040355	27.249039378735375	20.80802664410397
23	24.786723217124656	25.709620224513223	25.542420927262242	23.96123563109988
24	25.142947989027633	26.692876043479902	26.54911967751149	21.615056289980977
25	21.815533771293996	28.894221182709355	26.423551792710597	22.86669325328605
26	27.45032523698917	26.928383192226647	25.307317966985888	20.313973603798292
27	21.84194075135942	27.78122086474776	27.359382831151613	23.017455552741207
28	23.71225553334016	26.092790894442143	26.94657167339416	23.248381898823542
29	24.934386738306827	25.912253377668314	27.501118254768077	21.65224162925678
30	22.721454862926215	26.672801349450577	27.295925241300516	23.309818546322695
31	25.684695268839224	26.07783592103774	25.640503996076674	22.59696481404636
32	21.915233593989985	26.568116535619783	30.589926545481983	20.92672332490825
33	20.327311823321136	29.922207192399103	28.287669018145365	21.462811966134396
34	24.769882030858444	27.000328739955915	26.359555284898978	21.87023394428666
35	21.43627025657884	25.020209423519457	31.699962814660726	21.843557505240977
36	22.59117144597078	27.696341285966035	25.928959834444402	23.783527433618783
37	22.44701089153198	26.25783451985104	28.746422932037056	22.548731656579918
38	22.712832175557914	24.469569997359304	28.688354521791148	24.129243305291638
39	25.05416125503215	25.153456889257747	26.399031025506986	23.39335083020312
40	21.65224162925678	24.118464946081257	31.73957328475886	22.489720139903103
41	22.317401122027196	25.575294922853892	26.06395878355438	26.043345171564532
42	22.760930603534224	24.53720420140442	29.5459077264668	23.155957468594558
43	23.489143497685347	25.006062827055835	28.99526830030664	22.509525374952172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	25.5
2	37.0
3	113.0
4	189.0
5	189.0
6	255.0
7	321.0
8	312.5
9	304.0
10	460.5
11	617.0
12	617.0
13	948.5
14	1280.0
15	2940.0
16	4600.0
17	5155.0
18	5710.0
19	5710.0
20	5760.5
21	5811.0
22	7049.0
23	8287.0
24	10643.5
25	13000.0
26	13000.0
27	15667.0
28	18334.0
29	21305.5
30	24277.0
31	27533.5
32	30790.0
33	30790.0
34	33708.0
35	36626.0
36	38619.5
37	40613.0
38	42290.0
39	43967.0
40	43967.0
41	44651.0
42	45335.0
43	45615.0
44	45895.0
45	47335.0
46	48775.0
47	48775.0
48	51313.5
49	53852.0
50	64093.0
51	74334.0
52	59403.5
53	44473.0
54	44473.0
55	51792.0
56	59111.0
57	49582.5
58	40054.0
59	34081.5
60	28109.0
61	28109.0
62	25683.0
63	23257.0
64	20498.5
65	17740.0
66	14570.5
67	11401.0
68	11401.0
69	9478.5
70	7556.0
71	6496.5
72	5437.0
73	3359.5
74	1282.0
75	1282.0
76	841.5
77	401.0
78	307.5
79	214.0
80	167.0
81	120.0
82	120.0
83	85.0
84	50.0
85	41.0
86	32.0
87	22.0
88	12.0
89	12.0
90	10.5
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	742228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.63550137126929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48688961281387	40.117912328757484
2	10.629457800654162	10.33938018879129
3	2.7197525503335704	3.9682958667378463
4	1.0744817679821284	2.090318380003947
5	0.5864449624839413	1.4261022388530844
6	0.38062849926033526	1.1107234738632126
7	0.26544016236620344	0.9036870766526002
8	0.20613869680625946	0.8020527096953998
9	0.16613787023941284	0.7272178754263849
>10	1.2103868078792561	11.826280722337662
>50	0.15992774067325555	5.4085979325918405
>100	0.10290998916747898	9.341371077005922
>500	0.0061189723288753665	1.9603676675154675
>1k	0.003893891482011597	3.367606478249901
>5k	8.344053175739134E-4	2.984244487514403
>10k+	5.562702117159424E-4	3.6258414960036287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	14158	1.907500121256541	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	12646	1.7037891321804084	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	9423	1.2695559854923286	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	6757	0.9103671648065016	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	5881	0.792344131452869	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3380	0.45538567663844537	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3074	0.4141584526587518	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3003	0.4045926588595418	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2817	0.3795329736954144	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1764	0.23766282058882174	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1698	0.22877067424026043	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1510	0.20344153009587349	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1157	0.15588202008008323	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1154	0.15547783160969406	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1144	0.15413053670839688	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.15130121741567282	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1039	0.13998394024477653	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1022	0.13769353891257133	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	1010	0.13607678503101472	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	979	0.13190017083699349	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	963	0.129744498994918	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	878	0.11829249233389202	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	840	0.11317277170896274	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	784	0.10562792026169857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.3472949012971756E-4	0.0
13	0.0	0.0	0.0	1.3472949012971756E-4	0.0
14	0.0	0.0	0.0	1.3472949012971756E-4	0.0
15	0.0	0.0	0.0	1.3472949012971756E-4	0.0
16	0.0	0.0	0.0	2.6945898025943513E-4	0.0
17	0.0	0.0	0.0	5.389179605188703E-4	0.0
18	0.0	0.0	0.0	6.736474506485878E-4	0.0
19	0.0	0.0	0.0	6.736474506485878E-4	0.0
20	0.0	0.0	0.0	6.736474506485878E-4	0.0
21	0.0	0.0	0.0	9.431064309080229E-4	0.0
22	0.0	0.0	0.0	9.431064309080229E-4	0.0
23	0.0	0.0	0.0	0.0017514833716863283	0.0
24	0.0	0.0	0.0	0.0022904013322051983	0.0
25	0.0	0.0	0.0	0.002425130822334916	0.0
26	0.0	0.0	0.0	0.002425130822334916	0.0
27	0.0	0.0	0.0	0.0037724257236320916	0.0
28	2.6945898025943513E-4	0.0	0.0	0.011182547680766557	0.0
29	2.6945898025943513E-4	0.0	0.0	0.025598603124646336	0.0
30	2.6945898025943513E-4	0.0	0.0	0.04365235480202849	0.0
31	2.6945898025943513E-4	0.0	0.0	0.08838254552509471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	50	7.3250703E-9	33.300003	1
CTTATAC	1740	0.0	31.577587	1
CGTCTTC	430	0.0	31.406977	37
GGTATCA	610	0.0	30.32787	1
TTATACA	1890	0.0	29.36508	2
TATACAC	1970	0.0	28.82995	3
CCGTCTT	1510	0.0	26.096024	37
GCCGTCT	1555	0.0	25.459805	36
TTACGGG	85	1.9736035E-9	23.941175	3
GACGTTG	40	0.0019305459	23.125002	30
AGGACCG	40	0.0019305459	23.125002	5
GTATCAA	1650	0.0	22.98485	1
GCTTTAT	1000	0.0	22.755	1
TAGGACC	100	5.329639E-10	22.2	4
TTATTGA	1130	0.0	20.464602	4
TGCCGTC	1935	0.0	20.45995	35
CGGAAAT	55	5.140734E-4	20.181818	37
CTTTATT	1170	0.0	19.923077	2
TTTATTG	1205	0.0	19.80498	3
AACCACG	75	9.25852E-6	19.733334	35
>>END_MODULE