FastQCFastQC Report
Fri 10 Feb 2017
ERR1630887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369854
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT156314.226262254835692No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG121773.2923802365257644No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT116723.155839871949472RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC64221.7363608342751464No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC48631.314843154325761RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40201.086915377419198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38881.0512256187576718No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC36420.9847128867066464No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20110.543728065669156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15880.4293586117765389No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG14390.3890724448025437No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA13190.356627209655702No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11480.3103927495714525No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT10990.2971442785531588No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10830.2928182472002466No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10620.2871403310495493No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8810.23820210136972966No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8630.23333531609770342No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8450.22846853082567714No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8180.22116835291763776No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8080.21846458332206764No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7770.21008289757580018No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7450.20143083486997573No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5810.15708901350262536No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5780.15627788262395434No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5390.14573318120123074No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5390.14573318120123074No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5300.14329978856521763No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5030.13599961065717825No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4880.13194395626382302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.13113282538515197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4760.12869943274913884No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4690.12680679403223977No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4690.12680679403223977No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4410.11923623916464333No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4210.11382869997350305No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4060.10977304558014785No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4040.10923229166103382No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4040.10923229166103382No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3950.10679889902502068No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3890.10517663726767859No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3810.10301362159122249No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3700.10003947503609532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTT452.6102498E-937.02
CAGACAC251.2320072E-437.018
CTTATAC17800.034.609551
CGTCTTC5350.033.54205737
TTATACA18350.033.3705752
TGATACA451.07660526E-732.888892
GATTACG401.596185E-632.3751
TATACAC19050.032.1443563
GGTATCA4950.032.1414151
CTTAGGA601.2641976E-930.8333342
CTGTCCA250.00549158429.69
GATACAC502.7123497E-729.63
TCTCTGT250.00549158429.622
CTATACA250.00549158429.61
TCCTTGT250.00549158429.61
TGCTTGA453.998599E-628.77777725
CGATCAA405.929407E-527.7535
CCGTCTT18900.026.62433637
TAGACCT358.857978E-426.4285724
GCCTGTT358.857978E-426.42857211
Produced by FastQC (version 0.11.5)