##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630887.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 369854 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33632189999297 31.0 31.0 34.0 28.0 34.0 2 31.342813650791935 31.0 31.0 34.0 28.0 34.0 3 30.807126595900005 31.0 31.0 34.0 26.0 34.0 4 34.95680187317158 37.0 35.0 37.0 32.0 37.0 5 35.168999118571115 37.0 35.0 37.0 32.0 37.0 6 35.38708247038021 37.0 35.0 37.0 33.0 37.0 7 35.38674720295035 37.0 35.0 37.0 33.0 37.0 8 35.418343454444184 37.0 35.0 37.0 33.0 37.0 9 37.19894877438124 39.0 37.0 39.0 34.0 39.0 10 37.03525715552623 39.0 37.0 39.0 33.0 39.0 11 37.08149702314967 39.0 37.0 39.0 33.0 39.0 12 36.99738815857068 39.0 37.0 39.0 33.0 39.0 13 37.09829824741655 39.0 37.0 39.0 33.0 39.0 14 38.059723566596546 40.0 37.0 41.0 33.0 41.0 15 38.0781227186944 40.0 37.0 41.0 33.0 41.0 16 38.083614074743004 40.0 37.0 41.0 33.0 41.0 17 38.07427525455991 40.0 37.0 41.0 33.0 41.0 18 38.18192043346834 40.0 37.0 41.0 33.0 41.0 19 38.111227673622565 40.0 37.0 41.0 33.0 41.0 20 38.12257269084558 40.0 37.0 41.0 33.0 41.0 21 38.04691851379193 40.0 37.0 41.0 33.0 41.0 22 37.927214522487255 40.0 37.0 41.0 33.0 41.0 23 37.969820523774246 40.0 37.0 41.0 33.0 41.0 24 37.84686930518529 40.0 37.0 41.0 33.0 41.0 25 37.77717964385947 40.0 37.0 41.0 33.0 41.0 26 37.607756033461854 39.0 37.0 41.0 32.0 41.0 27 37.4876113277131 39.0 36.0 41.0 32.0 41.0 28 37.497455752810566 39.0 36.0 41.0 32.0 41.0 29 37.39818144457002 39.0 36.0 41.0 32.0 41.0 30 37.2002546950959 39.0 36.0 41.0 31.0 41.0 31 37.12007440773927 39.0 36.0 40.0 31.0 41.0 32 36.926151941036196 39.0 35.0 40.0 31.0 41.0 33 36.82542030098363 39.0 35.0 40.0 31.0 41.0 34 36.796752231961804 39.0 35.0 40.0 31.0 41.0 35 36.690445419003176 39.0 35.0 40.0 30.0 41.0 36 36.61345828353891 39.0 35.0 40.0 30.0 41.0 37 36.55202593455796 39.0 35.0 40.0 30.0 41.0 38 36.473619320056024 39.0 35.0 40.0 30.0 41.0 39 36.289070822540786 39.0 35.0 40.0 30.0 41.0 40 36.04943301951581 38.0 35.0 40.0 29.0 41.0 41 35.96663818696026 38.0 35.0 40.0 28.0 41.0 42 35.82016417288985 38.0 35.0 40.0 28.0 41.0 43 35.00797341653734 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 4.0 10 4.0 11 6.0 12 7.0 13 6.0 14 1.0 15 7.0 16 9.0 17 11.0 18 22.0 19 44.0 20 76.0 21 131.0 22 249.0 23 426.0 24 767.0 25 1293.0 26 2032.0 27 3080.0 28 4454.0 29 6070.0 30 7817.0 31 10015.0 32 12825.0 33 16169.0 34 21106.0 35 28172.0 36 37523.0 37 55292.0 38 83724.0 39 78509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.219697502257645 21.924056519599624 16.603849086396252 25.25239689174647 2 13.451524114921023 21.191605336159675 42.06389548308251 23.292975065836792 3 13.93441736468985 30.386314599815062 34.89890605482163 20.780361980673455 4 10.16887744893931 20.183910407890682 33.78981976671876 35.857392376451244 5 13.124638370816594 37.588075294575695 32.918935579985614 16.368350754622092 6 26.336067745650986 39.40149356232459 16.315086493589362 17.947352198435055 7 23.55659260140488 32.922720857419414 19.734273524147365 23.78641301702834 8 26.4039323624998 31.492426741362806 22.86659060061538 19.237050295522018 9 22.471299485743025 12.760710983252851 17.159744115245477 47.608245415758645 10 15.894920698437762 27.187484791296022 29.80527451372704 27.112319996539174 11 30.591530712118836 21.812931589221694 23.7558604205984 23.83967727806107 12 20.371552017823248 27.663077863156815 23.703677667403895 28.261692451616042 13 32.33573247822113 17.92896656518518 25.55359682469299 24.1817041319007 14 22.91012129110406 23.41978185986903 24.382053458932443 29.28804339009447 15 26.744877708501193 26.699995133214728 20.703304547199703 25.851822611084373 16 27.47489549930513 23.693673719900286 21.0750728665906 27.75635791420398 17 19.9686903480833 32.08130775927798 21.628804879763365 26.321197012875352 18 25.88480860015033 21.416288589551552 23.97513613479914 28.72376667549898 19 25.296738713113825 27.81584084530653 24.198467503393232 22.688952938186418 20 26.34120490788257 21.25271052901956 24.60727746624344 27.798807096854432 21 28.817317103505708 22.42641691045656 24.07842013334992 24.677845852687817 22 20.598127909932025 32.105912062597675 24.295262454914642 23.000697572555655 23 25.755568413482077 24.335007867969523 24.929566802035396 24.979856916513 24 27.13908731553532 28.261692451616042 23.756401174517514 20.842819058331123 25 22.10385719770504 32.04399573885912 24.54617227338355 21.30597479005229 26 26.964423799661486 30.30222736539283 23.89348229301292 18.83986654193276 27 23.11182250293359 30.50014329978857 25.527370259615957 20.86066393766189 28 22.15009165778929 25.078003752832203 28.57722236341908 24.194682225959433 29 22.66813391230053 27.140168823373546 24.68568678451497 25.506010479810953 30 23.995414406765914 27.18802554521514 28.562081253683886 20.254478794335064 31 30.758082919205957 22.750058131046305 23.354891389575346 23.13696756017239 32 21.322738161544827 26.766237488306192 26.978753778518012 24.93227057163097 33 19.205416191253846 30.579904502857886 29.83744937191432 20.377229933973947 34 23.55632222444532 27.868564352420144 26.408799147771823 22.166314275362712 35 20.234200522368287 27.59088721495509 33.379117165151655 18.79579509752497 36 23.352998750858447 27.593861361510218 23.812098828186258 25.24104105944508 37 21.399795595018574 25.806940035797908 28.45852687817355 24.334737491009967 38 21.75804506643162 21.52714314296993 29.528138130181098 27.186673660417355 39 26.002963331476746 21.974887387996343 26.862221308948936 25.15992797157797 40 19.60178881396443 21.977320780632358 36.50710821026675 21.91378219513646 41 23.275400563465585 22.93337370962596 25.228874096265013 28.562351630643445 42 23.43140806912998 21.99083962861021 32.398459932838364 22.179292369421447 43 23.984328951424075 20.976385276352293 31.49323787224148 23.546047899982156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 35.0 1 25.5 2 16.0 3 93.5 4 171.0 5 171.0 6 222.0 7 273.0 8 294.0 9 315.0 10 495.0 11 675.0 12 675.0 13 1056.0 14 1437.0 15 3707.5 16 5978.0 17 6079.0 18 6180.0 19 6180.0 20 5024.5 21 3869.0 22 3064.5 23 2260.0 24 2261.0 25 2262.0 26 2262.0 27 2637.0 28 3012.0 29 3680.0 30 4348.0 31 5390.0 32 6432.0 33 6432.0 34 7941.5 35 9451.0 36 10467.5 37 11484.0 38 13623.0 39 15762.0 40 15762.0 41 17371.0 42 18980.0 43 21188.5 44 23397.0 45 26040.5 46 28684.0 47 28684.0 48 32062.5 49 35441.0 50 46817.0 51 58193.0 52 43597.5 53 29002.0 54 29002.0 55 37281.5 56 45561.0 57 36840.5 58 28120.0 59 19468.0 60 10816.0 61 10816.0 62 8936.5 63 7057.0 64 5494.5 65 3932.0 66 3271.5 67 2611.0 68 2611.0 69 2141.0 70 1671.0 71 1393.5 72 1116.0 73 819.0 74 522.0 75 522.0 76 384.0 77 246.0 78 179.0 79 112.0 80 89.0 81 66.0 82 66.0 83 67.0 84 68.0 85 96.5 86 125.0 87 106.5 88 88.0 89 88.0 90 85.0 91 82.0 92 43.0 93 4.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 369854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.20121453330233 #Duplication Level Percentage of deduplicated Percentage of total 1 68.44408753262398 13.826536957826601 2 10.89205648129559 4.400655393749966 3 4.8745231881148365 2.954138660119939 4 2.7986348122866893 2.2614328897348686 5 1.9527537977648397 1.97239991996842 6 1.4053402931138326 1.7033748452091908 7 1.0934885899752393 1.5462858317065653 8 0.8418657565415245 1.3605368604908965 9 0.7173927591514422 1.3042984529030375 >10 5.88502977983002 24.16764453000373 >50 0.6879475339623904 9.547821572836849 >100 0.36806531486314664 12.764766637646208 >500 0.01739945124807602 2.4352852747300284 >1k 0.01606103192130094 7.343978975487625 >5k 0.0013384193267750785 1.7363608342751464 >10k+ 0.004015257980325235 10.674482363310927 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT 15631 4.226262254835692 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG 12177 3.2923802365257644 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 11672 3.155839871949472 RNA PCR Primer, Index 17 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC 6422 1.7363608342751464 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC 4863 1.314843154325761 RNA PCR Primer, Index 17 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 1.086915377419198 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3888 1.0512256187576718 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 3642 0.9847128867066464 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 2011 0.543728065669156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1588 0.4293586117765389 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG 1439 0.3890724448025437 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA 1319 0.356627209655702 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 1148 0.3103927495714525 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT 1099 0.2971442785531588 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 1083 0.2928182472002466 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 1062 0.2871403310495493 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 881 0.23820210136972966 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 863 0.23333531609770342 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 845 0.22846853082567714 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 818 0.22116835291763776 No Hit ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 808 0.21846458332206764 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 777 0.21008289757580018 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 745 0.20143083486997573 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 581 0.15708901350262536 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 578 0.15627788262395434 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 539 0.14573318120123074 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 539 0.14573318120123074 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 530 0.14329978856521763 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 503 0.13599961065717825 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 488 0.13194395626382302 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.13113282538515197 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 476 0.12869943274913884 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 469 0.12680679403223977 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 469 0.12680679403223977 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 441 0.11923623916464333 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 421 0.11382869997350305 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 406 0.10977304558014785 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 404 0.10923229166103382 No Hit ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 404 0.10923229166103382 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 395 0.10679889902502068 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 389 0.10517663726767859 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 381 0.10301362159122249 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 370 0.10003947503609532 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.703769595570144E-4 0.0 9 0.0 0.0 0.0 5.407539191140287E-4 0.0 10 0.0 0.0 0.0 5.407539191140287E-4 0.0 11 0.0 0.0 0.0 5.407539191140287E-4 0.0 12 0.0 0.0 0.0 8.111308786710431E-4 0.0 13 0.0 0.0 0.0 8.111308786710431E-4 0.0 14 0.0 0.0 0.0 8.111308786710431E-4 0.0 15 0.0 0.0 0.0 8.111308786710431E-4 0.0 16 0.0 0.0 0.0 8.111308786710431E-4 0.0 17 0.0 0.0 0.0 8.111308786710431E-4 0.0 18 0.0 0.0 0.0 8.111308786710431E-4 0.0 19 0.0 0.0 0.0 8.111308786710431E-4 0.0 20 0.0 0.0 0.0 0.0010815078382280575 0.0 21 0.0 0.0 0.0 0.002163015676456115 0.0 22 0.0 0.0 0.0 0.003514900474241187 0.0 23 0.0 0.0 0.0 0.004055654393355216 0.0 24 0.0 0.0 0.0 0.006218670069811331 0.0 25 0.0 0.0 0.0 0.008111308786710432 0.0 26 0.0 0.0 0.0 0.009733570544052519 0.0 27 0.0 0.0 0.0 0.011355832301394605 0.0 28 0.0 0.0 0.0 0.022441287643232194 0.0 29 2.703769595570144E-4 0.0 0.0 0.044612198326907375 0.0 30 2.703769595570144E-4 0.0 0.0 0.07759818739286313 0.0 31 2.703769595570144E-4 0.0 0.0 0.1373514954549633 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACTT 45 2.6102498E-9 37.0 2 CAGACAC 25 1.2320072E-4 37.0 18 CTTATAC 1780 0.0 34.60955 1 CGTCTTC 535 0.0 33.542057 37 TTATACA 1835 0.0 33.370575 2 TGATACA 45 1.07660526E-7 32.88889 2 GATTACG 40 1.596185E-6 32.375 1 TATACAC 1905 0.0 32.144356 3 GGTATCA 495 0.0 32.141415 1 CTTAGGA 60 1.2641976E-9 30.833334 2 CTGTCCA 25 0.005491584 29.6 9 GATACAC 50 2.7123497E-7 29.6 3 TCTCTGT 25 0.005491584 29.6 22 CTATACA 25 0.005491584 29.6 1 TCCTTGT 25 0.005491584 29.6 1 TGCTTGA 45 3.998599E-6 28.777777 25 CGATCAA 40 5.929407E-5 27.75 35 CCGTCTT 1890 0.0 26.624336 37 TAGACCT 35 8.857978E-4 26.428572 4 GCCTGTT 35 8.857978E-4 26.428572 11 >>END_MODULE