FastQCFastQC Report
Fri 10 Feb 2017
ERR1630886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794745
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT203342.558556518128456No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG160162.0152375919320034No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT132421.666194817205519TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC86141.0838696688875047No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC48660.612271860785535RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC40110.5046901836438104TruSeq Adapter, Index 11 (95% over 21bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG39970.5029286123221913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23020.2896526558833336No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG22030.2771958301090287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.25177887246852765No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA19970.25127556637663656No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG19700.24787825025637156No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG15550.1956602432226689No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA15230.19163379448754003No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC14910.18760734575241114No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT12430.15640236805516233No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC12140.15275339888895179No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT12040.151495133659224No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT10880.13689925699438182No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC10810.1360184713335724No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG10790.13576681828762685No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC10590.1332502878281713No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC10470.131740369552498No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA10040.12632982906466855No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10020.12607817601872298No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9960.12532321688088632No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG9720.12230338032953966No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT9130.1148796154741458No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC8850.11135647283090802No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT8660.10896576889442525No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA8340.10493932015929637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8130.10229696317686804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24050.034.769231
TTATACA26250.032.0666662
CGTCTTC5450.031.22935737
TATACAC27600.030.6992763
GGTATCA3850.028.3506491
CCGTCTT18700.026.81016237
GCCGTCT19550.025.73913236
GTATCAA12200.024.5655731
TCGCCGA400.001930665823.1251
GATTACG400.001930665823.1251
TGCCGTC23300.021.75536335
ATGCCGT24400.020.69876934
GCGGGTA450.003824785420.55555522
TGCGTTT450.003824785420.55555536
CGCGATA1450.020.41379434
TCGTACT555.141173E-420.18181826
TATGCCG26250.019.31047633
GTTCTAT801.6156479E-518.51
CGCGCGA1601.8189894E-1218.532
CGTAGAC609.233395E-418.53
Produced by FastQC (version 0.11.5)