FastQCFastQC Report
Fri 10 Feb 2017
ERR1630885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618227
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT123631.9997509005591798No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG99591.610896968265702No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT96861.566738431029379TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC50510.8170138153137925No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC37800.6114259001952357RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC26150.4229837907435295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22730.3676643045353891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.35537108537802453No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG11560.18698633349886046No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA10350.16741423457726692No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT8720.141048514542393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7900.12778477808313127No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7490.12115290985350041No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT6740.10902144357978541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13600.033.4632341
CGTCTTC4650.031.03225737
TTATACA15600.029.4102572
TATACAC16000.029.2531263
CGGATCG405.9357575E-527.7537
GCAACGC358.8647415E-426.428573
TTACGGG706.5792847E-926.428573
TATCGAA358.8647415E-426.4285722
GCCGTCT14700.025.4217736
CCGTCTT14800.025.2537
CCAACGG601.3357367E-624.66666829
GGTATCA3650.024.3287681
ATTACGG701.9199251E-723.7857132
GATTACG701.9199251E-723.7857131
AGCAACG400.001930183323.1252
GTATCAA9650.023.0051821
ACGGAAC705.0956314E-621.14285732
TGCCGTC17750.021.0535235
TAACGCA450.003823838220.5555554
GGACCGT450.003823838220.5555556
Produced by FastQC (version 0.11.5)