##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630885.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618227
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405862248009225	31.0	31.0	34.0	28.0	34.0
2	31.40270483172039	31.0	31.0	34.0	28.0	34.0
3	30.857201319256518	31.0	31.0	34.0	27.0	34.0
4	35.026595409129655	37.0	35.0	37.0	32.0	37.0
5	35.21234271553976	37.0	35.0	37.0	32.0	37.0
6	35.4069249644548	37.0	35.0	37.0	33.0	37.0
7	35.4132850878399	37.0	35.0	37.0	33.0	37.0
8	35.440228265669404	37.0	35.0	37.0	33.0	37.0
9	37.20334602015117	39.0	37.0	39.0	34.0	39.0
10	37.03639601634998	39.0	37.0	39.0	33.0	39.0
11	37.09211179712306	39.0	37.0	39.0	33.0	39.0
12	37.0197273816899	39.0	37.0	39.0	33.0	39.0
13	37.10309320039403	39.0	37.0	39.0	33.0	39.0
14	38.197361163456144	40.0	38.0	41.0	33.0	41.0
15	38.19870047733276	40.0	38.0	41.0	33.0	41.0
16	38.202828410923495	40.0	38.0	41.0	33.0	41.0
17	38.175196165809645	40.0	38.0	41.0	33.0	41.0
18	38.221438080187376	40.0	38.0	41.0	33.0	41.0
19	38.23416803213059	40.0	38.0	41.0	34.0	41.0
20	38.21903604986518	40.0	38.0	41.0	34.0	41.0
21	38.167045438002546	40.0	38.0	41.0	33.0	41.0
22	38.081434489273356	40.0	37.0	41.0	33.0	41.0
23	38.11800196368001	40.0	37.0	41.0	33.0	41.0
24	38.06925773219222	40.0	37.0	41.0	33.0	41.0
25	38.0165942283336	40.0	37.0	41.0	33.0	41.0
26	37.90953808229016	40.0	37.0	41.0	33.0	41.0
27	37.810343126392084	40.0	37.0	41.0	33.0	41.0
28	37.79069015102867	40.0	37.0	41.0	33.0	41.0
29	37.73377739891658	40.0	37.0	41.0	33.0	41.0
30	37.65364178529893	40.0	37.0	41.0	32.0	41.0
31	37.62880786507221	39.0	37.0	41.0	32.0	41.0
32	37.51934807117774	39.0	37.0	41.0	32.0	41.0
33	37.522972953300325	39.0	37.0	41.0	32.0	41.0
34	37.46016430857921	39.0	37.0	41.0	32.0	41.0
35	37.44236340373358	39.0	36.0	41.0	32.0	41.0
36	37.42436677789873	39.0	36.0	41.0	32.0	41.0
37	37.356778335465776	39.0	36.0	41.0	32.0	41.0
38	37.30261052978922	39.0	36.0	41.0	31.0	41.0
39	37.21946631253569	39.0	36.0	41.0	31.0	41.0
40	37.12589226934443	39.0	36.0	41.0	31.0	41.0
41	37.0933411190388	39.0	36.0	41.0	31.0	41.0
42	37.033494169617306	39.0	36.0	41.0	31.0	41.0
43	36.34150886324926	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	17.0
11	13.0
12	8.0
13	9.0
14	9.0
15	5.0
16	7.0
17	12.0
18	15.0
19	28.0
20	64.0
21	123.0
22	237.0
23	447.0
24	838.0
25	1444.0
26	2326.0
27	3719.0
28	5619.0
29	7788.0
30	10632.0
31	14409.0
32	18612.0
33	24021.0
34	31613.0
35	42617.0
36	60072.0
37	88834.0
38	153556.0
39	151125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.16596007615326	22.9420584995479	15.723189055152881	24.16879236914596
2	13.302718904221264	22.324000731123032	41.865366604823144	22.507913759832554
3	14.578302144681485	28.80204196840319	35.13385859886417	21.485797288051153
4	11.298277170036249	19.82556569027234	37.93913885999803	30.93701827969338
5	11.989770747638003	38.83557334118374	35.89231787029683	13.282338040881426
6	26.908239206634455	40.86185171466145	17.816433122461493	14.41347595624261
7	23.308428781014094	34.1892217583509	22.993172410781153	19.509177049853857
8	24.426626465683317	35.787502001691934	22.48866516667826	17.297206365946487
9	23.30956105119964	15.06550183023388	19.912265235908492	41.712671882657986
10	13.658575248250237	29.488359453728165	34.12743215679678	22.725633141224826
11	30.258626685667238	23.318942718451314	25.9071506097275	20.515279986153953
12	19.538292568910773	27.304048512924865	29.67647805741257	23.481180860751795
13	28.551648504513714	21.078988785672575	27.477932862848114	22.891429846965597
14	21.124117840210797	23.208627251802334	27.123532294771984	28.54372261321489
15	23.428449420682046	29.6824629141076	23.26750530145076	23.62158236375959
16	24.363219335292698	28.174926038493954	23.858550338306156	23.603304287907193
17	20.067548004212046	31.446216357422113	25.428523827008526	23.05771181135732
18	22.780467368782016	26.005981621637357	26.482182111101586	24.731368898479037
19	23.157836846336377	27.547163097050113	26.524561366617768	22.770438689995746
20	23.382835107492877	26.039302715669166	27.09053470650748	23.487327470330477
21	22.9943046809667	26.857448801168506	27.396247656605098	22.751998861259697
22	20.334925520884724	32.33423968865805	26.385130380911864	20.94570440954536
23	21.88888547410579	25.786159452757644	27.5872778121952	24.737677260941368
24	22.566630056597333	28.64627394144869	27.980660825230864	20.806435176723113
25	22.181172934860495	29.538502847659515	26.555132661627525	21.72519155585246
26	23.91839890525648	26.72254689620479	27.388321765306273	21.970732433232453
27	21.873195444391786	30.81246855928324	26.695857670402617	20.618478325922354
28	20.15279177389535	26.08863734518227	30.035245953347236	23.723324927575145
29	20.571408236780343	28.259199290875358	27.553633212396093	23.615759259948206
30	22.23568365665039	27.306313053295955	28.340075732700125	22.11792755735353
31	22.32966208205077	25.802819999773547	30.094932767413912	21.772585150761774
32	21.075430222232285	30.564016129997558	27.844464897197955	20.5160887505722
33	21.658711120672503	26.193776719554467	29.476874998989043	22.670637160783986
34	20.32182353730911	28.972853013537097	29.409424046507187	21.2958994026466
35	20.914486103001	28.233318829491434	30.860994424378102	19.991200643129464
36	24.392981865884213	26.4077757846228	26.09219590862256	23.107046440870423
37	21.56748249429417	27.650523189702163	28.296725959882053	22.48526835612162
38	21.533190882960465	25.696548355215803	29.198498286228197	23.571762475595534
39	23.60864213306763	26.139427750648224	27.440406193841422	22.811523922442728
40	20.24240287143719	25.517973171666718	32.658554220375365	21.58106973652073
41	21.700119858886787	26.733060833642014	27.18046930981662	24.386349997654584
42	22.302002338946696	25.505356446742056	30.56353087134661	21.629110342964637
43	21.99596588308178	25.207731140826912	30.545414548377863	22.250888427713445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	15.0
2	20.0
3	73.0
4	126.0
5	126.0
6	189.5
7	253.0
8	246.5
9	240.0
10	397.0
11	554.0
12	554.0
13	779.5
14	1005.0
15	2562.5
16	4120.0
17	4641.5
18	5163.0
19	5163.0
20	5597.0
21	6031.0
22	7392.5
23	8754.0
24	11042.0
25	13330.0
26	13330.0
27	16088.0
28	18846.0
29	22319.0
30	25792.0
31	27906.5
32	30021.0
33	30021.0
34	32523.0
35	35025.0
36	36425.5
37	37826.0
38	39671.5
39	41517.0
40	41517.0
41	42092.0
42	42667.0
43	41231.5
44	39796.0
45	40165.0
46	40534.0
47	40534.0
48	41155.0
49	41776.0
50	51773.0
51	61770.0
52	50011.0
53	38252.0
54	38252.0
55	42168.5
56	46085.0
57	38539.0
58	30993.0
59	23611.0
60	16229.0
61	16229.0
62	13939.5
63	11650.0
64	9744.5
65	7839.0
66	6477.0
67	5115.0
68	5115.0
69	4079.0
70	3043.0
71	2486.5
72	1930.0
73	1448.0
74	966.0
75	966.0
76	717.5
77	469.0
78	374.0
79	279.0
80	199.0
81	119.0
82	119.0
83	79.5
84	40.0
85	30.0
86	20.0
87	19.5
88	19.0
89	19.0
90	11.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	618227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.39778623799526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.06821069908776	49.093973978160726
2	10.151839463758968	11.856899018540814
3	2.478447527458993	4.3420754673191455
4	0.9312565769145881	2.1753329004554125
5	0.48782819728556814	1.4244043392974592
6	0.31513450415173505	1.1041894445801792
7	0.22919504449886932	0.9369138250816947
8	0.15213748292693252	0.7107593765402926
9	0.126945941541549	0.667202576213198
>10	0.9113285893492707	10.4500774582635
>50	0.09993111690565604	3.9999242330032674
>100	0.0438586469010771	4.614298802965133
>500	0.0011103454911663114	0.5000917550376244
>1k	0.001665518236749467	2.116433696522277
>5k	8.327591183747335E-4	4.003327709046733
>10k+	2.7758637279157785E-4	2.004095418972496
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	12363	1.9997509005591798	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	9959	1.610896968265702	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	9686	1.566738431029379	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	5051	0.8170138153137925	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	3780	0.6114259001952357	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	2615	0.4229837907435295	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2273	0.3676643045353891	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2197	0.35537108537802453	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1156	0.18698633349886046	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1035	0.16741423457726692	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	872	0.141048514542393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.12778477808313127	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	749	0.12115290985350041	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	674	0.10902144357978541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.6175288364953327E-4	0.0
13	0.0	0.0	0.0	1.6175288364953327E-4	0.0
14	0.0	0.0	0.0	3.2350576729906654E-4	0.0
15	0.0	0.0	0.0	4.852586509485998E-4	0.0
16	0.0	0.0	0.0	4.852586509485998E-4	0.0
17	0.0	0.0	0.0	6.470115345981331E-4	0.0
18	0.0	0.0	0.0	6.470115345981331E-4	0.0
19	0.0	0.0	0.0	6.470115345981331E-4	0.0
20	0.0	0.0	0.0	9.705173018971996E-4	0.0
21	0.0	0.0	0.0	0.0012940230691962662	0.0
22	0.0	0.0	0.0	0.0016175288364953327	0.0
23	0.0	0.0	0.0	0.0017792817201448658	0.0
24	0.0	0.0	0.0	0.0021027874874439325	0.0
25	0.0	0.0	0.0	0.0021027874874439325	0.0
26	0.0	0.0	0.0	0.0025880461383925323	0.0
27	0.0	0.0	0.0	0.003720316323939265	0.0
28	0.0	0.0	0.0	0.00841114994977573	0.0
29	0.0	0.0	0.0	0.020542616223490726	0.0
30	0.0	0.0	0.0	0.03526212863559825	0.0
31	0.0	0.0	0.0	0.0744063264787853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1360	0.0	33.463234	1
CGTCTTC	465	0.0	31.032257	37
TTATACA	1560	0.0	29.410257	2
TATACAC	1600	0.0	29.253126	3
CGGATCG	40	5.9357575E-5	27.75	37
GCAACGC	35	8.8647415E-4	26.42857	3
TTACGGG	70	6.5792847E-9	26.42857	3
TATCGAA	35	8.8647415E-4	26.42857	22
GCCGTCT	1470	0.0	25.42177	36
CCGTCTT	1480	0.0	25.25	37
CCAACGG	60	1.3357367E-6	24.666668	29
GGTATCA	365	0.0	24.328768	1
ATTACGG	70	1.9199251E-7	23.785713	2
GATTACG	70	1.9199251E-7	23.785713	1
AGCAACG	40	0.0019301833	23.125	2
GTATCAA	965	0.0	23.005182	1
ACGGAAC	70	5.0956314E-6	21.142857	32
TGCCGTC	1775	0.0	21.05352	35
TAACGCA	45	0.0038238382	20.555555	4
GGACCGT	45	0.0038238382	20.555555	6
>>END_MODULE