FastQCFastQC Report
Fri 10 Feb 2017
ERR1630884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703073
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG31160.4431972213411694No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT29720.4227157066193695No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT23080.32827316651329236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22050.3136231941775605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.3113474703195827No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC19010.270384440875983No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC10200.14507739594608243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACC303.59807E-430.8333328
CCGGTCG303.59807E-430.8333329
GGCGTAA250.005494910829.59999815
TATATCG358.8659575E-426.428575
GGTATCA4050.025.5802461
CTTATAC5400.025.3518521
GGTTCGC451.322498E-424.66666823
GATTACG551.9012963E-523.5454541
CCGTCTT4550.023.17582337
ATACCGC400.001930445723.1256
TATACAC6450.022.9457363
GCCGTCT4750.022.236
CCGCCGT502.7006568E-422.19999924
TTATACA7350.020.8911552
TACCGCA806.952214E-720.81257
CGTTATG450.00382435220.55555712
GTATCAA9450.020.3597891
ATAATAC1551.8189894E-1219.0967753
CTAGAAC1103.8453436E-818.53
GACTATA609.2319545E-418.51
Produced by FastQC (version 0.11.5)