##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630884.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703073
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65242016120659	31.0	31.0	34.0	30.0	34.0
2	31.63945422452576	33.0	31.0	34.0	30.0	34.0
3	31.130100288305766	31.0	31.0	34.0	27.0	34.0
4	35.25600328842098	37.0	35.0	37.0	32.0	37.0
5	35.44446451506458	37.0	35.0	37.0	33.0	37.0
6	35.62536180453523	37.0	35.0	37.0	33.0	37.0
7	35.63800771754853	37.0	35.0	37.0	33.0	37.0
8	35.66316015548883	37.0	35.0	37.0	33.0	37.0
9	37.431396170810146	39.0	37.0	39.0	34.0	39.0
10	37.28187257937654	39.0	37.0	39.0	34.0	39.0
11	37.339381543595046	39.0	37.0	39.0	34.0	39.0
12	37.28794591742251	39.0	37.0	39.0	34.0	39.0
13	37.344076646379534	39.0	37.0	39.0	34.0	39.0
14	38.566207207501925	40.0	38.0	41.0	34.0	41.0
15	38.57064202437016	40.0	38.0	41.0	34.0	41.0
16	38.57263043809107	40.0	38.0	41.0	34.0	41.0
17	38.556255182605504	40.0	38.0	41.0	34.0	41.0
18	38.57871231010151	40.0	38.0	41.0	34.0	41.0
19	38.612321906829024	40.0	38.0	41.0	34.0	41.0
20	38.60059339499597	40.0	38.0	41.0	34.0	41.0
21	38.57518209346682	40.0	38.0	41.0	34.0	41.0
22	38.514407465512114	40.0	38.0	41.0	34.0	41.0
23	38.497154634013825	40.0	38.0	41.0	34.0	41.0
24	38.46910918211907	40.0	38.0	41.0	34.0	41.0
25	38.43982772770395	40.0	38.0	41.0	34.0	41.0
26	38.342842066186584	40.0	38.0	41.0	34.0	41.0
27	38.28652216768387	40.0	38.0	41.0	34.0	41.0
28	38.25177470902737	40.0	38.0	41.0	34.0	41.0
29	38.171248220312826	40.0	38.0	41.0	34.0	41.0
30	38.10818648988085	40.0	38.0	41.0	33.0	41.0
31	38.06896012220637	40.0	37.0	41.0	33.0	41.0
32	37.991295356243235	40.0	37.0	41.0	33.0	41.0
33	37.99083309983458	40.0	37.0	41.0	33.0	41.0
34	37.92244759790235	40.0	37.0	41.0	33.0	41.0
35	37.88070371070998	40.0	37.0	41.0	33.0	41.0
36	37.85451155143207	40.0	37.0	41.0	33.0	41.0
37	37.80513545535101	40.0	37.0	41.0	33.0	41.0
38	37.77386843186981	40.0	37.0	41.0	33.0	41.0
39	37.705204153764974	40.0	37.0	41.0	33.0	41.0
40	37.64992255427246	40.0	37.0	41.0	33.0	41.0
41	37.598098632716656	40.0	37.0	41.0	32.0	41.0
42	37.53575802228218	40.0	37.0	41.0	32.0	41.0
43	36.98755463515168	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	2.0
12	4.0
13	2.0
14	5.0
15	2.0
16	6.0
17	6.0
18	7.0
19	15.0
20	33.0
21	88.0
22	210.0
23	370.0
24	665.0
25	1152.0
26	1934.0
27	3118.0
28	4755.0
29	6868.0
30	9772.0
31	13020.0
32	17632.0
33	23320.0
34	31865.0
35	42818.0
36	60360.0
37	92331.0
38	167241.0
39	225462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.518190856426	22.947261521918776	15.330413769267203	23.20413385238802
2	13.89116066183739	22.888946098058096	40.579285508048244	22.640607732056274
3	14.81496231543524	28.23433697496561	33.7572343127954	23.193466396803746
4	12.05010006073338	18.74997333136104	40.21900997478213	28.980916633123442
5	12.563275790707365	38.72044581430378	36.01219219056912	12.70408620441974
6	27.990550056679748	40.570324845357455	18.294828559765484	13.144296538197315
7	24.638124348396254	33.399376736128396	24.348111789245213	17.614387126230135
8	24.347827323762967	36.28343002789184	21.974816270856653	17.39392637748854
9	24.700422289008394	15.888677278177374	21.1106101357896	38.30029029702463
10	14.225407603477874	29.165819196584135	34.79581778848	21.812955411457985
11	32.1662188705867	23.961665431612364	25.748535358348278	18.123580339452662
12	20.901954704561263	26.347619663960923	31.871797096460824	20.87862853501699
13	27.544223715033862	22.26553999371331	27.322198406139904	22.868037885112926
14	21.203772581225564	21.832299064250797	28.701144831333302	28.262783523190336
15	22.490125491947495	29.851381008799937	24.51153720879624	23.146956290456323
16	22.551001105148398	29.004811733632213	25.50560183650915	22.938585324710235
17	21.215720131479944	29.33137810725202	27.46912482772059	21.983776933547443
18	22.36936989473355	27.782605789157028	27.990550056679748	21.857474259429672
19	22.918530508211806	28.140321133082907	27.97831804094312	20.96283031776217
20	22.63349609500009	27.276968394462596	27.7956912013404	22.29384430919691
21	23.062754507711148	27.20343406730169	28.04616305845908	21.687648366528084
22	21.457800256872332	28.68990844478454	28.12424883333594	21.72804246500719
23	22.428823180523217	26.95025978810166	28.047443153129194	22.573473878245927
24	22.326984537878715	27.815888250579956	28.02383251810267	21.833294693438663
25	21.992168665273734	27.68105161199477	28.108176533588974	22.21860318914252
26	22.78468949881449	27.38392741578755	28.07361397749594	21.757769107902025
27	21.773983640390114	28.388232800861363	27.721587943215	22.11619561553352
28	22.1877386843187	27.295174185326417	28.09679791429908	22.4202892160558
29	22.29427100742028	27.78232132367478	28.163362837144934	21.760044831760002
30	21.840548563235966	27.150807953085952	28.400180351115743	22.60846313256234
31	23.05692296532508	27.03218584698886	27.6011168114833	22.309774376202757
32	21.74141234267281	27.76895144600916	28.702424926003417	21.787211285314612
33	21.514835586062897	27.197460292174497	28.702424926003417	22.58527919575919
34	22.344052466813547	27.66369921757769	27.94403995033233	22.048208365276437
35	21.775548200542474	27.279670816543945	29.48797635522912	21.456804627684463
36	22.56010400058031	27.22718693506933	27.36301920284238	22.84968986150798
37	22.371645618591526	27.255633483294055	28.301328596034836	22.071392302079584
38	22.174226573911955	27.18025013049854	28.22637194146269	22.41915135412681
39	22.556690414793344	27.121934706637862	27.95641419881008	22.364960679758717
40	21.783228768563152	27.017251409170882	29.324693168419213	21.874826653846753
41	22.18261830563825	27.07514013480819	27.86510077900872	22.877140780544835
42	22.070254440150595	27.0794071170419	28.712665683364317	22.137672759443188
43	22.44105519625985	26.520432444426113	28.681374480317125	22.35713787899692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	19.5
2	32.0
3	92.5
4	153.0
5	153.0
6	183.0
7	213.0
8	226.0
9	239.0
10	345.5
11	452.0
12	452.0
13	728.5
14	1005.0
15	2402.0
16	3799.0
17	4483.0
18	5167.0
19	5167.0
20	5555.5
21	5944.0
22	7348.5
23	8753.0
24	11367.5
25	13982.0
26	13982.0
27	17264.0
28	20546.0
29	24248.0
30	27950.0
31	31515.0
32	35080.0
33	35080.0
34	39352.5
35	43625.0
36	46251.5
37	48878.0
38	50887.5
39	52897.0
40	52897.0
41	53860.5
42	54824.0
43	54740.0
44	54656.0
45	53554.0
46	52452.0
47	52452.0
48	50943.0
49	49434.0
50	47594.5
51	45755.0
52	45902.5
53	46050.0
54	46050.0
55	40243.0
56	34436.0
57	32642.0
58	30848.0
59	27184.5
60	23521.0
61	23521.0
62	19569.5
63	15618.0
64	12973.0
65	10328.0
66	8633.0
67	6938.0
68	6938.0
69	5574.5
70	4211.0
71	3385.5
72	2560.0
73	1919.0
74	1278.0
75	1278.0
76	974.0
77	670.0
78	522.5
79	375.0
80	290.0
81	205.0
82	205.0
83	157.5
84	110.0
85	81.0
86	52.0
87	36.0
88	20.0
89	20.0
90	13.5
91	7.0
92	3.5
93	0.0
94	1.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	703073.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.69849894047447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02478525452406	53.88905267136994
2	10.928216217553203	14.3593480318039
3	3.115217917756348	6.139954232071876
4	1.3365996981432282	3.5125037540920547
5	0.7044790940854201	2.314160950817869
6	0.43165354738547923	1.701539407533417
7	0.27059078588622204	1.2444185921893678
8	0.20198467793139055	1.0616072119254032
9	0.14446874173710697	0.8542241528352302
>10	0.7829002212346204	9.15386558827557
>50	0.04503164666558797	2.0210278352887183
>100	0.011690748358735268	1.1674582201904178
>500	8.659813594771134E-4	0.3461980149798532
>1k	0.0015154673790849483	2.2346413366263245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3116	0.4431972213411694	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	2972	0.4227157066193695	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2308	0.32827316651329236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2205	0.3136231941775605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2189	0.3113474703195827	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	1901	0.270384440875983	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	1020	0.14507739594608243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.422327411236102E-4	0.0	0.0	0.0
6	0.0	1.422327411236102E-4	0.0	0.0	0.0
7	0.0	1.422327411236102E-4	0.0	0.0	0.0
8	0.0	1.422327411236102E-4	0.0	0.0	0.0
9	0.0	1.422327411236102E-4	0.0	0.0	0.0
10	0.0	1.422327411236102E-4	0.0	1.422327411236102E-4	0.0
11	0.0	1.422327411236102E-4	0.0	1.422327411236102E-4	0.0
12	0.0	1.422327411236102E-4	0.0	2.844654822472204E-4	0.0
13	0.0	2.844654822472204E-4	0.0	2.844654822472204E-4	0.0
14	0.0	2.844654822472204E-4	0.0	2.844654822472204E-4	0.0
15	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
16	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
17	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
18	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
19	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
20	0.0	2.844654822472204E-4	0.0	4.266982233708306E-4	0.0
21	0.0	2.844654822472204E-4	0.0	5.689309644944408E-4	0.0
22	0.0	2.844654822472204E-4	0.0	7.111637056180511E-4	0.0
23	0.0	2.844654822472204E-4	0.0	0.0011378619289888817	0.0
24	0.0	2.844654822472204E-4	0.0	0.0018490256346069328	0.0
25	0.0	2.844654822472204E-4	0.0	0.001991258375730543	0.0
26	0.0	2.844654822472204E-4	0.0	0.002133491116854153	0.0
27	0.0	2.844654822472204E-4	0.0	0.002702422081348594	0.0
28	0.0	2.844654822472204E-4	0.0	0.0069694043150569	0.0
29	0.0	2.844654822472204E-4	0.0	0.01422327411236102	0.0
30	0.0	2.844654822472204E-4	0.0	0.025317427920002616	0.0
31	0.0	2.844654822472204E-4	0.0	0.05305281243910661	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTACC	30	3.59807E-4	30.833332	8
CCGGTCG	30	3.59807E-4	30.833332	9
GGCGTAA	25	0.0054949108	29.599998	15
TATATCG	35	8.8659575E-4	26.42857	5
GGTATCA	405	0.0	25.580246	1
CTTATAC	540	0.0	25.351852	1
GGTTCGC	45	1.322498E-4	24.666668	23
GATTACG	55	1.9012963E-5	23.545454	1
CCGTCTT	455	0.0	23.175823	37
ATACCGC	40	0.0019304457	23.125	6
TATACAC	645	0.0	22.945736	3
GCCGTCT	475	0.0	22.2	36
CCGCCGT	50	2.7006568E-4	22.199999	24
TTATACA	735	0.0	20.891155	2
TACCGCA	80	6.952214E-7	20.8125	7
CGTTATG	45	0.003824352	20.555557	12
GTATCAA	945	0.0	20.359789	1
ATAATAC	155	1.8189894E-12	19.096775	3
CTAGAAC	110	3.8453436E-8	18.5	3
GACTATA	60	9.2319545E-4	18.5	1
>>END_MODULE