FastQCFastQC Report
Fri 10 Feb 2017
ERR1630883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886231
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT252832.8528679317243473No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG194352.192994828662053No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT154931.7481898060437966TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC105721.1929169708574854No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC56310.6353873877126843RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC48750.5500823148817859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33450.3774410960573485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30190.34065610433397164No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG25210.2844630801675861No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA22110.24948348681100074No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA18530.20908769835404087No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15880.17918578790405662No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT15330.17297973101821082No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT13340.15052508883124152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11840.13359947914257117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC32050.034.575661
TTATACA34000.032.5926482
CGTCTTC6200.032.22580737
GATTACG1100.031.9545481
TATACAC35850.031.3751753
GATCGTC250.00549567229.617
GGTATCA5650.028.8141571
AGCAACG454.006897E-628.7777772
GCAACGC454.006897E-628.7777773
TTACGGG1250.028.1200033
CCGTCTT22000.026.48863837
TAGACTC358.867784E-426.4285725
GCCGTCT22100.026.28506736
GTATCAA17900.025.0111731
ATTACGG1750.021.1428592
GCTTTAT5200.020.9903851
TGCCGTC27950.020.84973135
GCTCGGA806.956416E-720.812511
GGGCGTT450.003825127620.55555525
ACGGGCT555.141815E-420.1818215
Produced by FastQC (version 0.11.5)