##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630883.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	886231
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454083641849586	31.0	31.0	34.0	28.0	34.0
2	31.455201860463017	31.0	31.0	34.0	28.0	34.0
3	30.941375329908343	31.0	31.0	34.0	27.0	34.0
4	35.087072106482395	37.0	35.0	37.0	32.0	37.0
5	35.282251467168265	37.0	35.0	37.0	33.0	37.0
6	35.46656684318197	37.0	35.0	37.0	33.0	37.0
7	35.46843091699568	37.0	35.0	37.0	33.0	37.0
8	35.49510680623901	37.0	35.0	37.0	33.0	37.0
9	37.27806294295731	39.0	37.0	39.0	34.0	39.0
10	37.10970503175808	39.0	37.0	39.0	33.0	39.0
11	37.15717346831695	39.0	37.0	39.0	33.0	39.0
12	37.09188123638194	39.0	37.0	39.0	33.0	39.0
13	37.1812766648876	39.0	37.0	39.0	33.0	39.0
14	38.26876062787242	40.0	38.0	41.0	33.0	41.0
15	38.273574271267876	40.0	38.0	41.0	33.0	41.0
16	38.245657170647384	40.0	38.0	41.0	33.0	41.0
17	38.213263810451224	40.0	38.0	41.0	33.0	41.0
18	38.27490349581542	40.0	38.0	41.0	33.0	41.0
19	38.280459609289224	40.0	38.0	41.0	34.0	41.0
20	38.33542270581823	40.0	38.0	41.0	34.0	41.0
21	38.26903143762743	40.0	38.0	41.0	34.0	41.0
22	38.19265405972032	40.0	38.0	41.0	34.0	41.0
23	38.19979102513904	40.0	38.0	41.0	34.0	41.0
24	38.104823685923876	40.0	37.0	41.0	33.0	41.0
25	38.107810491846934	40.0	37.0	41.0	33.0	41.0
26	37.93716198146984	40.0	37.0	41.0	33.0	41.0
27	37.86440329891417	40.0	37.0	41.0	33.0	41.0
28	37.8984395716241	40.0	37.0	41.0	33.0	41.0
29	37.820157498440025	40.0	37.0	41.0	33.0	41.0
30	37.80236981103121	40.0	37.0	41.0	33.0	41.0
31	37.75727095982876	40.0	37.0	41.0	33.0	41.0
32	37.66467659109194	40.0	37.0	41.0	33.0	41.0
33	37.611357535450686	40.0	37.0	41.0	32.0	41.0
34	37.50881090821693	40.0	37.0	41.0	32.0	41.0
35	37.50551041432764	39.0	37.0	41.0	32.0	41.0
36	37.48182471612932	39.0	37.0	41.0	32.0	41.0
37	37.39841643995753	39.0	36.0	41.0	32.0	41.0
38	37.37484019403519	39.0	36.0	41.0	32.0	41.0
39	37.30278223172062	39.0	36.0	41.0	31.0	41.0
40	37.18499465714921	39.0	36.0	41.0	31.0	41.0
41	37.16331746463394	39.0	36.0	41.0	31.0	41.0
42	37.08839794590801	39.0	36.0	41.0	31.0	41.0
43	36.407322695775704	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	23.0
11	31.0
12	20.0
13	15.0
14	11.0
15	17.0
16	14.0
17	19.0
18	18.0
19	50.0
20	85.0
21	167.0
22	377.0
23	679.0
24	1172.0
25	2040.0
26	3229.0
27	5188.0
28	7615.0
29	10855.0
30	14977.0
31	19784.0
32	25886.0
33	33615.0
34	44480.0
35	59887.0
36	83046.0
37	123368.0
38	212009.0
39	237536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.04417922640937	22.591288275855845	15.37996301190096	24.98456948583383
2	13.133031907031011	21.701339718425558	41.77657969536159	23.38904867918184
3	14.02636558639903	28.75130750334845	35.356131753459316	21.866195156793207
4	10.872785989205974	19.829592961654466	37.421168972875016	31.87645207626454
5	12.17278565069378	37.97542627148001	35.92596061297788	13.925827464848329
6	26.67047304822332	40.86451500793811	17.278113719786376	15.186898224052195
7	23.61551333681625	33.63129928878588	22.039626237403116	20.713561136994755
8	24.839799104296738	34.389905115032086	23.03485208709693	17.735443693574247
9	23.371784557299392	14.543273706291023	19.155728021249537	42.92921371516004
10	14.28713281300248	29.012187567349823	33.200260428714415	23.50041919093329
11	30.613350243898036	22.85092712848005	25.486808743995642	21.048913883626277
12	20.09983852968357	26.812873844404	27.977919977974143	25.109367647938292
13	29.65513506072345	19.989709229309288	26.885879640861134	23.469276069106133
14	21.398596979794206	22.943792306971883	26.156047351085665	29.501563362148243
15	24.280689797580994	28.57482981299458	22.209108009085668	24.935372380338762
16	25.289230460229895	26.886330990452826	23.084049192591998	24.740389356725277
17	20.38091648791342	31.62392197970958	24.215582618978573	23.779578913398424
18	24.289603952017025	25.320824931648744	25.434565028756612	24.95500608757762
19	23.51001036975687	28.544363715554972	25.82870605970678	22.116919854981376
20	22.259772000753753	25.234391484838604	26.54567488611886	25.960161628288787
21	24.47634984558202	25.30897700486668	25.438175825490195	24.776497324061108
22	20.106721610956964	31.39418503753536	27.834503645212138	20.664589706295537
23	22.71292699081842	25.314280362569125	25.527655882044296	26.445136764568154
24	23.547021036276096	29.82224724704958	26.045918050711382	20.584813665962937
25	23.26312214309813	27.92635328712266	26.320451439861614	22.490073129917594
26	25.4635642400232	28.470906569506145	24.90411642111368	21.161412769356975
27	20.996557330989322	29.372364541524725	29.209427338921795	20.421650788564154
28	20.63660603161027	25.246803598610295	27.20983581030228	26.906754559477157
29	20.849643038891667	29.46150608588506	27.98446454705376	21.704386328169516
30	22.03138910735463	24.690515226842663	30.365446480657976	22.912649185144733
31	22.686071690112396	26.749459226770444	25.178649810263913	25.38581927285324
32	23.605357971003045	25.91423680733353	29.796745995118656	20.683659226544773
33	22.012093912309545	25.502380304909217	28.812352535625585	23.673173247155653
34	21.3100196224235	30.91880108007957	26.114748863445307	21.656430434051618
35	24.03368873352433	24.941239925030835	30.903568031359768	20.121503310085068
36	25.56331249978843	25.382772663109282	25.204828086582392	23.8490867505199
37	21.820608848031718	27.022864241941434	28.531500252191584	22.62502665783526
38	21.64074603574012	24.32559908195493	29.350474086327377	24.68318079597757
39	24.582529837028947	25.086010306567925	26.669570349039923	23.661889507363203
40	20.526476731235988	24.128584985178808	33.66571469515284	21.67922358843236
41	22.293171870539396	25.603595450847465	25.997962156593484	26.10527052201965
42	22.935216664729623	24.220208952293476	30.701250576881193	22.1433238060957
43	22.8396433886876	24.01224962791868	30.621023186956897	22.52708379643682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	20.0
2	25.0
3	117.0
4	209.0
5	209.0
6	261.5
7	314.0
8	316.5
9	319.0
10	467.5
11	616.0
12	616.0
13	1021.0
14	1426.0
15	3186.0
16	4946.0
17	5782.0
18	6618.0
19	6618.0
20	6866.0
21	7114.0
22	8673.0
23	10232.0
24	13440.0
25	16648.0
26	16648.0
27	19787.5
28	22927.0
29	26497.0
30	30067.0
31	34034.0
32	38001.0
33	38001.0
34	41366.5
35	44732.0
36	47618.0
37	50504.0
38	52425.0
39	54346.0
40	54346.0
41	55010.5
42	55675.0
43	56292.5
44	56910.0
45	57288.5
46	57667.0
47	57667.0
48	59904.5
49	62142.0
50	81155.0
51	100168.0
52	76547.0
53	52926.0
54	52926.0
55	64063.5
56	75201.0
57	62597.0
58	49993.0
59	38869.5
60	27746.0
61	27746.0
62	24514.0
63	21282.0
64	18150.0
65	15018.0
66	12354.0
67	9690.0
68	9690.0
69	7879.0
70	6068.0
71	5031.0
72	3994.0
73	2725.0
74	1456.0
75	1456.0
76	1028.0
77	600.0
78	458.0
79	316.0
80	242.5
81	169.0
82	169.0
83	120.5
84	72.0
85	55.0
86	38.0
87	32.5
88	27.0
89	27.0
90	19.5
91	12.0
92	6.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	886231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63329281944699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07254873925443	39.74264258374856
2	12.247490860192316	12.15766600334852
3	3.271152858512251	4.870742630511288
4	1.3030957494750919	2.5870773162189593
5	0.6691430080030641	1.6605885427150822
6	0.4227309293283489	1.2588916799514538
7	0.2913643652333231	1.012296099974448
8	0.2025106584347045	0.8041016647318963
9	0.1622407886256994	0.7247290112205611
>10	1.1365249762938634	11.370613142059296
>50	0.13745219090347557	4.761301462213354
>100	0.0773729830253423	6.789043084348377
>500	0.002958378761961684	0.9792703600612419
>1k	0.002275675970739757	2.6501292339235203
>5k	2.275675970739757E-4	0.6360174622266267
>10k+	9.102703882959028E-4	7.994889722746816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	25283	2.8528679317243473	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	19435	2.192994828662053	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	15493	1.7481898060437966	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	10572	1.1929169708574854	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	5631	0.6353873877126843	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	4875	0.5500823148817859	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3345	0.3774410960573485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.34065610433397164	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2521	0.2844630801675861	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2211	0.24948348681100074	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1853	0.20908769835404087	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1588	0.17918578790405662	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1533	0.17297973101821082	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1334	0.15052508883124152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.13359947914257117	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.128373979244689E-4	0.0
10	0.0	0.0	0.0	1.128373979244689E-4	0.0
11	0.0	0.0	0.0	1.128373979244689E-4	0.0
12	0.0	0.0	0.0	2.256747958489378E-4	0.0
13	0.0	0.0	0.0	2.256747958489378E-4	0.0
14	0.0	0.0	0.0	2.256747958489378E-4	0.0
15	0.0	0.0	0.0	2.256747958489378E-4	0.0
16	0.0	0.0	0.0	2.256747958489378E-4	0.0
17	0.0	0.0	0.0	4.513495916978756E-4	0.0
18	0.0	0.0	0.0	6.770243875468135E-4	0.0
19	0.0	0.0	0.0	6.770243875468135E-4	0.0
20	0.0	0.0	0.0	6.770243875468135E-4	0.0
21	0.0	0.0	0.0	9.026991833957512E-4	0.0
22	0.0	0.0	0.0	0.001354048775093627	0.0
23	0.0	0.0	0.0	0.00203107316264044	0.0
24	0.0	0.0	0.0	0.0025952601522627847	0.0
25	0.0	0.0	0.0	0.002708097550187254	0.0
26	0.0	0.0	0.0	0.003159447141885129	0.0
27	0.0	0.0	0.0	0.004287821121129819	0.0
28	1.128373979244689E-4	0.0	0.0	0.010832390200749015	0.0
29	1.128373979244689E-4	0.0	0.0	0.022228967391120374	0.0
30	1.128373979244689E-4	0.0	0.0	0.04174983723205349	0.0
31	1.128373979244689E-4	0.0	0.0	0.09026991833957512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3205	0.0	34.57566	1
TTATACA	3400	0.0	32.592648	2
CGTCTTC	620	0.0	32.225807	37
GATTACG	110	0.0	31.954548	1
TATACAC	3585	0.0	31.375175	3
GATCGTC	25	0.005495672	29.6	17
GGTATCA	565	0.0	28.814157	1
AGCAACG	45	4.006897E-6	28.777777	2
GCAACGC	45	4.006897E-6	28.777777	3
TTACGGG	125	0.0	28.120003	3
CCGTCTT	2200	0.0	26.488638	37
TAGACTC	35	8.867784E-4	26.428572	5
GCCGTCT	2210	0.0	26.285067	36
GTATCAA	1790	0.0	25.011173	1
ATTACGG	175	0.0	21.142859	2
GCTTTAT	520	0.0	20.990385	1
TGCCGTC	2795	0.0	20.849731	35
GCTCGGA	80	6.956416E-7	20.8125	11
GGGCGTT	45	0.0038251276	20.555555	25
ACGGGCT	55	5.141815E-4	20.18182	15
>>END_MODULE