FastQCFastQC Report
Fri 10 Feb 2017
ERR1630882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179698
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT66950.5675181275207722No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG49570.42019228650044327No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT39020.33076261890755093RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27410.23234760082665226No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC26940.22836353032725326No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC25520.21632655137162224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.20361143275651905No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC12870.10909571771758535RNA PCR Primer, Index 29 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11600.025.9956913
GTATCAA16000.025.5531251
CTTATAC11550.025.1471841
GATTACG1200.024.6666681
TTATACA11750.024.5617032
GGTATCA5000.023.311
CCGTCTT5950.021.76470637
GCCGTCT5950.021.76470636
CGGATCG806.9604357E-720.812537
AAGTCGC759.266114E-619.73333229
GCTAGTC801.6167294E-518.57
TGCCGTC7100.018.23943735
CGTCTTC2700.017.81481637
ATGCCGT7300.017.73972534
GTACTAG852.7232136E-517.4117641
TTACGGG1950.017.0769233
TGCGTTA650.001579967317.07692337
ATACACA18100.016.762434
TATGCCG7750.016.70967933
TTGACGC1005.8780533E-616.65000232
Produced by FastQC (version 0.11.5)