##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1179698
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.544076534841967	31.0	31.0	34.0	30.0	34.0
2	31.52380778809492	31.0	31.0	34.0	30.0	34.0
3	30.997147575057344	31.0	31.0	34.0	27.0	34.0
4	35.148278627241886	37.0	35.0	37.0	32.0	37.0
5	35.340691431196795	37.0	35.0	37.0	33.0	37.0
6	35.52873616807013	37.0	35.0	37.0	33.0	37.0
7	35.54661955856499	37.0	35.0	37.0	33.0	37.0
8	35.568750646351866	37.0	35.0	37.0	33.0	37.0
9	37.33357605081979	39.0	37.0	39.0	34.0	39.0
10	37.17930945038476	39.0	37.0	39.0	33.0	39.0
11	37.23743110524897	39.0	37.0	39.0	34.0	39.0
12	37.17855756303732	39.0	37.0	39.0	33.0	39.0
13	37.24404296692882	39.0	37.0	39.0	34.0	39.0
14	38.43488163919918	40.0	38.0	41.0	34.0	41.0
15	38.43476126940963	40.0	38.0	41.0	34.0	41.0
16	38.44674145416878	40.0	38.0	41.0	34.0	41.0
17	38.41452303894726	40.0	38.0	41.0	34.0	41.0
18	38.427833225113545	40.0	38.0	41.0	34.0	41.0
19	38.46374919682834	40.0	38.0	41.0	34.0	41.0
20	38.4435694559116	40.0	38.0	41.0	34.0	41.0
21	38.41472902386882	40.0	38.0	41.0	34.0	41.0
22	38.36698375346911	40.0	38.0	41.0	34.0	41.0
23	38.342632605972035	40.0	38.0	41.0	34.0	41.0
24	38.3248178771177	40.0	38.0	41.0	34.0	41.0
25	38.31044216401147	40.0	38.0	41.0	34.0	41.0
26	38.19646299307111	40.0	38.0	41.0	34.0	41.0
27	38.15771324525429	40.0	38.0	41.0	33.0	41.0
28	38.11100807155729	40.0	38.0	41.0	33.0	41.0
29	38.04338059401643	40.0	37.0	41.0	33.0	41.0
30	37.97551407224561	40.0	37.0	41.0	33.0	41.0
31	37.954487504429096	40.0	37.0	41.0	33.0	41.0
32	37.89993625487201	40.0	37.0	41.0	33.0	41.0
33	37.8714272635878	40.0	37.0	41.0	33.0	41.0
34	37.811423771168556	40.0	37.0	41.0	33.0	41.0
35	37.77879931982592	40.0	37.0	41.0	33.0	41.0
36	37.74239169685801	40.0	37.0	41.0	33.0	41.0
37	37.68112262629927	40.0	37.0	41.0	33.0	41.0
38	37.65334433049814	40.0	37.0	41.0	33.0	41.0
39	37.589926404893454	40.0	37.0	41.0	32.0	41.0
40	37.55176918160411	40.0	37.0	41.0	32.0	41.0
41	37.47979652419517	40.0	37.0	41.0	32.0	41.0
42	37.435591142817906	40.0	37.0	41.0	32.0	41.0
43	36.85447546745014	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	4.0
11	9.0
12	5.0
13	8.0
14	3.0
15	3.0
16	9.0
17	8.0
18	14.0
19	29.0
20	77.0
21	159.0
22	322.0
23	750.0
24	1217.0
25	2186.0
26	3553.0
27	5755.0
28	8505.0
29	12722.0
30	17534.0
31	23882.0
32	31498.0
33	41481.0
34	55658.0
35	75945.0
36	106300.0
37	159650.0
38	283964.0
39	348441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04126479827888	21.117692833250544	14.569661048844704	24.27138131962587
2	14.719190843758318	21.739885970816257	40.39839009644841	23.14253308897701
3	15.174392090179012	28.020561194475196	32.7109989166719	24.0940477986739
4	12.401309487682441	18.21364450901841	39.869186859687815	29.515859143611333
5	12.663919070813037	38.43017450228787	36.0014173118883	12.904489115010792
6	28.739897838260305	40.00608630344376	17.97036190618277	13.283653952113166
7	25.955032559180403	32.25537383296403	24.133464666380718	17.65612894147485
8	24.85670061320779	35.91342869107178	21.282226468129977	17.94764422759045
9	25.22230265712072	15.594753911594323	20.308672219500245	38.87427121178471
10	14.872619941713896	28.602065952472582	34.15594499609222	22.3693691097213
11	32.426434562065886	23.799904721377843	25.168814391479856	18.60484632507642
12	21.95553438252841	25.937231393119255	30.229431600290923	21.877802624061413
13	27.635208332980138	21.503893369319947	27.010641706606265	23.85025659109365
14	21.83770761669512	21.196611336121617	27.6144403059088	29.35124074127446
15	23.366743013889995	29.128471863137857	23.960708588130185	23.544076534841967
16	23.31435672519577	27.814152435623356	24.789140949632873	24.082349889548002
17	21.922983678873745	28.667930266898818	26.296221575352334	23.112864478875103
18	23.140583437456026	27.072267648160803	27.05667043599294	22.730478478390232
19	24.21636723975119	26.700308044940314	27.034122292315494	22.049202422993
20	23.59112247371785	26.887728893326933	26.670215597551238	22.850933035403976
21	23.736583430674628	26.681404901932527	26.518227546372035	23.063784121020802
22	22.750314063429794	27.957155136314547	26.867384703542776	22.425146096712886
23	23.258579738204187	26.316565765136502	26.9982656578209	23.426588838838413
24	22.91323711661798	27.34208246517329	26.72785746860637	23.016822949602357
25	23.17084541975997	27.020559499126044	27.309277459146326	22.499317621967656
26	23.266802181575287	27.157374175424557	27.35623863056477	22.219585012435385
27	23.256630086683202	27.60503111813362	26.40548682798479	22.732851967198382
28	22.68275439985488	26.376581124999788	27.682593341685752	23.25807113345958
29	23.19737763393682	26.63732582406684	27.685729737610814	22.479566804385527
30	23.796937860367652	26.544081620889415	27.278506872097775	22.380473646645157
31	22.916288745085605	26.572733021502117	26.776853059003237	23.734125174409044
32	22.473294012535412	27.079388114585257	27.56154541246997	22.88577246040936
33	23.09633482467547	27.213151162416143	27.185093134005484	22.505420878902907
34	23.014958065538806	26.899681104825135	27.015642986594873	23.069717843041186
35	23.0553073752774	26.51288719655369	28.52119779808053	21.91060763008838
36	23.832285890117642	27.38344898440109	25.69683088383637	23.087434241644896
37	22.90408223121511	26.658178618595606	27.394807823697253	23.042931326492035
38	23.493470362753857	26.37276658941526	27.101258118603234	23.03250492922765
39	22.901793509864387	26.667163969083614	27.102360095549876	23.32868242550212
40	22.739548596335673	25.928246042631248	28.65826677675134	22.67393858428174
41	22.525002161570164	26.167459807510056	27.201453253290246	24.10608477762953
42	22.614686131535358	26.521872547041703	27.598334488996336	23.265106832426603
43	23.229334965389448	25.397262689264537	28.287409150477494	23.085993194868518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	19.5
2	24.0
3	85.5
4	147.0
5	147.0
6	183.5
7	220.0
8	235.5
9	251.0
10	380.5
11	510.0
12	510.0
13	771.5
14	1033.0
15	2553.0
16	4073.0
17	4885.5
18	5698.0
19	5698.0
20	5894.0
21	6090.0
22	8099.5
23	10109.0
24	13720.0
25	17331.0
26	17331.0
27	22109.5
28	26888.0
29	32286.0
30	37684.0
31	44285.5
32	50887.0
33	50887.0
34	58620.5
35	66354.0
36	71811.0
37	77268.0
38	82142.0
39	87016.0
40	87016.0
41	90483.0
42	93950.0
43	94843.5
44	95737.0
45	94110.5
46	92484.0
47	92484.0
48	88250.0
49	84016.0
50	87257.5
51	90499.0
52	80387.0
53	70275.0
54	70275.0
55	72466.0
56	74657.0
57	64838.5
58	55020.0
59	46182.5
60	37345.0
61	37345.0
62	34602.0
63	31859.0
64	25559.5
65	19260.0
66	15676.5
67	12093.0
68	12093.0
69	10152.5
70	8212.0
71	7229.0
72	6246.0
73	7024.0
74	7802.0
75	7802.0
76	6720.0
77	5638.0
78	3924.5
79	2211.0
80	1342.5
81	474.0
82	474.0
83	335.0
84	196.0
85	138.0
86	80.0
87	55.5
88	31.0
89	31.0
90	20.0
91	9.0
92	7.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1179698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51021109812272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20173692355912	39.21294714423393
2	12.071256613236725	11.953009262819203
3	3.506452621703681	5.208156285183453
4	1.4802736625250184	2.9315464605841965
5	0.8114677471479096	2.0087969730305537
6	0.519168829316584	1.542249500101759
7	0.33701478954484615	1.1679971361488313
8	0.25065101498303727	0.9927827731015115
9	0.1891255418512788	0.8427280950993291
>10	1.3377108164986795	13.282204269947867
>50	0.17032018549132089	5.819648201252838
>100	0.11827889972085058	10.864566124630702
>500	0.004820682204937885	1.6635507654159654
>1k	0.00154950499444432	1.9391328326730781
>5k	1.7216722160492447E-4	0.5706841757767928
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	6695	0.5675181275207722	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	4957	0.42019228650044327	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	3902	0.33076261890755093	RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2741	0.23234760082665226	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2694	0.22836353032725326	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	2552	0.21632655137162224	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2402	0.20361143275651905	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	1287	0.10909571771758535	RNA PCR Primer, Index 29 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.476745743402125E-5	0.0
5	0.0	0.0	0.0	8.476745743402125E-5	0.0
6	0.0	0.0	0.0	8.476745743402125E-5	0.0
7	0.0	0.0	0.0	8.476745743402125E-5	0.0
8	0.0	0.0	0.0	8.476745743402125E-5	0.0
9	0.0	0.0	0.0	2.543023723020637E-4	0.0
10	0.0	0.0	0.0	2.543023723020637E-4	8.476745743402125E-5
11	8.476745743402125E-5	0.0	0.0	3.39069829736085E-4	8.476745743402125E-5
12	8.476745743402125E-5	0.0	0.0	3.39069829736085E-4	8.476745743402125E-5
13	8.476745743402125E-5	0.0	0.0	3.39069829736085E-4	8.476745743402125E-5
14	8.476745743402125E-5	0.0	0.0	3.39069829736085E-4	8.476745743402125E-5
15	1.695349148680425E-4	0.0	0.0	4.2383728717010626E-4	8.476745743402125E-5
16	1.695349148680425E-4	0.0	0.0	5.933722020381488E-4	8.476745743402125E-5
17	2.543023723020637E-4	0.0	0.0	5.933722020381488E-4	8.476745743402125E-5
18	2.543023723020637E-4	0.0	0.0	6.7813965947217E-4	8.476745743402125E-5
19	2.543023723020637E-4	0.0	0.0	8.476745743402125E-4	8.476745743402125E-5
20	2.543023723020637E-4	0.0	0.0	8.476745743402125E-4	8.476745743402125E-5
21	2.543023723020637E-4	0.0	0.0	9.324420317742338E-4	8.476745743402125E-5
22	2.543023723020637E-4	0.0	0.0	0.0011019769466422762	8.476745743402125E-5
23	2.543023723020637E-4	0.0	0.0	0.0015258142338123826	8.476745743402125E-5
24	2.543023723020637E-4	0.0	0.0	0.0019496515209824886	8.476745743402125E-5
25	2.543023723020637E-4	0.0	0.0	0.0019496515209824886	8.476745743402125E-5
26	2.543023723020637E-4	0.0	0.0	0.0019496515209824886	8.476745743402125E-5
27	2.543023723020637E-4	0.0	0.0	0.002373488808152595	8.476745743402125E-5
28	3.39069829736085E-4	0.0	0.0	0.0067813965947217	1.695349148680425E-4
29	3.39069829736085E-4	0.0	0.0	0.015427677252991868	1.695349148680425E-4
30	3.39069829736085E-4	0.0	0.0	0.030770587048549715	1.695349148680425E-4
31	3.39069829736085E-4	0.0	0.0	0.07315431576556033	1.695349148680425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1160	0.0	25.995691	3
GTATCAA	1600	0.0	25.553125	1
CTTATAC	1155	0.0	25.147184	1
GATTACG	120	0.0	24.666668	1
TTATACA	1175	0.0	24.561703	2
GGTATCA	500	0.0	23.31	1
CCGTCTT	595	0.0	21.764706	37
GCCGTCT	595	0.0	21.764706	36
CGGATCG	80	6.9604357E-7	20.8125	37
AAGTCGC	75	9.266114E-6	19.733332	29
GCTAGTC	80	1.6167294E-5	18.5	7
TGCCGTC	710	0.0	18.239437	35
CGTCTTC	270	0.0	17.814816	37
ATGCCGT	730	0.0	17.739725	34
GTACTAG	85	2.7232136E-5	17.411764	1
TTACGGG	195	0.0	17.076923	3
TGCGTTA	65	0.0015799673	17.076923	37
ATACACA	1810	0.0	16.76243	4
TATGCCG	775	0.0	16.709679	33
TTGACGC	100	5.8780533E-6	16.650002	32
>>END_MODULE