FastQCFastQC Report
Fri 10 Feb 2017
ERR1630881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878586
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT163951.8660666115781495No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG130301.4830648337214571No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT124711.4194398727045503TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC69190.7875153940536271No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC50270.5721693721502505RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC34440.3919934986444128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25070.2853448609470217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.23674404099314122No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG16470.18746030553639598No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA13560.1543389036474517No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT10200.11609563548702119No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9910.11279487722317451No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9330.10619336069548115No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT8960.10198204842781469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC6750.033.43703537
CTTATAC19550.032.0792851
CGCCGTT250.00549564929.59999825
TTATACA22000.028.8431842
GGTATCA4250.028.729411
TATACAC23000.027.6695653
CCGTCTT21100.025.25118437
GCCGTCT21500.024.6953536
GTATCAA12850.024.1867711
TGCCGTC24600.021.5081335
GCACCGC806.95627E-720.812510
TTAACGG806.95627E-720.812535
ATGCCGT25950.020.3892134
GGCACCG851.2446253E-619.5882369
TATGCCG28100.019.224233
TCTATAC1355.638867E-1119.1851863
CGTCAGA609.234451E-418.512
AGCCGGT500.00703359718.49999831
GCGACTC500.00703359718.49999820
GTCGCCA1451.546141E-1017.86206812
Produced by FastQC (version 0.11.5)