##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878586
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.407589012344836	31.0	31.0	34.0	28.0	34.0
2	31.405756522412148	31.0	31.0	34.0	28.0	34.0
3	30.861517256136565	31.0	31.0	34.0	27.0	34.0
4	35.030978185402454	37.0	35.0	37.0	32.0	37.0
5	35.223880189304175	37.0	35.0	37.0	32.0	37.0
6	35.41357818130496	37.0	35.0	37.0	33.0	37.0
7	35.430208311992224	37.0	35.0	37.0	33.0	37.0
8	35.46357328707719	37.0	35.0	37.0	33.0	37.0
9	37.21361483110361	39.0	37.0	39.0	34.0	39.0
10	37.051403049900635	39.0	37.0	39.0	33.0	39.0
11	37.104468999050745	39.0	37.0	39.0	33.0	39.0
12	37.03870082154735	39.0	37.0	39.0	33.0	39.0
13	37.120170364654115	39.0	37.0	39.0	33.0	39.0
14	38.22535870136788	40.0	38.0	41.0	33.0	41.0
15	38.242947190144164	40.0	38.0	41.0	33.0	41.0
16	38.2415722535984	40.0	38.0	41.0	33.0	41.0
17	38.19579301286385	40.0	38.0	41.0	33.0	41.0
18	38.25205728295238	40.0	38.0	41.0	33.0	41.0
19	38.28794563082043	40.0	38.0	41.0	34.0	41.0
20	38.28571818808859	40.0	38.0	41.0	34.0	41.0
21	38.24697980618858	40.0	38.0	41.0	34.0	41.0
22	38.1678902236093	40.0	38.0	41.0	33.0	41.0
23	38.15985230814058	40.0	38.0	41.0	33.0	41.0
24	38.11239764803901	40.0	37.0	41.0	33.0	41.0
25	38.084723635477914	40.0	37.0	41.0	33.0	41.0
26	37.956377634062	40.0	37.0	41.0	33.0	41.0
27	37.89806234107987	40.0	37.0	41.0	33.0	41.0
28	37.8767098496903	40.0	37.0	41.0	33.0	41.0
29	37.84606060192172	40.0	37.0	41.0	33.0	41.0
30	37.75855636215464	40.0	37.0	41.0	33.0	41.0
31	37.72873230395203	40.0	37.0	41.0	33.0	41.0
32	37.64514458459388	40.0	37.0	41.0	32.0	41.0
33	37.56922145356288	40.0	37.0	41.0	32.0	41.0
34	37.53135378892903	40.0	37.0	41.0	32.0	41.0
35	37.51522560113637	40.0	37.0	41.0	32.0	41.0
36	37.46978895634577	39.0	37.0	41.0	32.0	41.0
37	37.42623032918804	39.0	37.0	41.0	32.0	41.0
38	37.39606936600401	39.0	37.0	41.0	32.0	41.0
39	37.29517998238078	39.0	36.0	41.0	31.0	41.0
40	37.19495757956535	39.0	36.0	41.0	31.0	41.0
41	37.16841037758398	39.0	36.0	41.0	31.0	41.0
42	37.11332072216038	39.0	36.0	41.0	31.0	41.0
43	36.433886950167654	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	17.0
11	18.0
12	17.0
13	12.0
14	14.0
15	9.0
16	19.0
17	15.0
18	24.0
19	45.0
20	87.0
21	189.0
22	350.0
23	622.0
24	1202.0
25	2015.0
26	3397.0
27	5202.0
28	7733.0
29	10670.0
30	14807.0
31	19826.0
32	25844.0
33	33294.0
34	44342.0
35	59407.0
36	83056.0
37	123805.0
38	215910.0
39	226629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.70046415490344	22.47622884953778	15.416931296424027	24.406375699134745
2	13.587514483499625	22.277500438204115	41.53571761899234	22.599267459303928
3	14.585595490936573	28.63476085437282	34.96902978194508	21.810613872745524
4	11.62162838925273	19.621869686063743	37.69409027687671	31.062411647806815
5	12.072466440393997	38.6960411388299	36.00455732278912	13.226935097986992
6	27.050396887726414	40.414484182538764	18.02020519334476	14.514913736390064
7	23.768532619458995	33.92280323155616	22.854108761122987	19.45455538786186
8	24.234963907915674	35.8217636065223	22.309597466838763	17.633675018723267
9	23.528829277953438	15.465076839375996	19.53502559794943	41.47106828472113
10	13.82539671699754	29.31153011771187	34.03229735051549	22.8307758147751
11	30.389967515985916	23.519040822412375	25.711996321361823	20.378995340239886
12	19.92633618109098	26.977780205921785	29.413512166139682	23.682371446847547
13	28.432162588522925	21.12246268435873	27.29328716824534	23.152087558873006
14	21.502618980953486	22.92194503440756	26.614355339147224	28.961080645491734
15	23.756012501906472	29.266685333023744	23.217988904899464	23.75931326017032
16	24.551836701244955	28.140785307300593	23.56582053435862	23.741557457095833
17	20.642600724345712	31.210490492678005	25.011325015422507	23.135583767553776
18	23.550568754794636	26.35234342454808	26.018966839899566	24.078120980757717
19	24.619331516778097	27.323221631120916	26.197776882399676	21.859669969701315
20	22.262476297141088	25.87817242705893	26.71110170205307	25.148249573746906
21	23.491268925295874	26.217012335730367	26.88183057776928	23.40988816120448
22	20.380816448247526	30.99639648252988	25.78051551014926	22.84227155907333
23	22.23368002677029	27.423951667793478	27.144866865622717	23.19750143981352
24	24.207419649300125	27.70975180574241	27.14429776937033	20.938530775587136
25	22.70728192800705	28.70282476615835	25.957162986890296	22.6327303189443
26	24.233939534661374	28.098558365373457	26.805457860698894	20.862044239266275
27	21.07932518842777	29.33224522129877	26.258442542904163	23.329987047369297
28	20.294427637135122	27.253564249828706	29.449365230040087	23.00264288299609
29	23.08482038184082	26.32844138194781	26.963438980361627	23.623299255849737
30	21.150689858477143	27.992023546926536	27.783620499302287	23.07366609529403
31	21.772370604585095	26.483804658849557	29.488974329206247	22.254850407359097
32	23.06148743549294	26.731247709387585	27.34962769723169	22.85763715788779
33	20.552683516468505	29.62919964579449	28.820741509653008	20.99737532808399
34	20.980302440512368	28.4828121549854	28.79524599754606	21.74163940695618
35	23.21263940012702	26.246150063852596	30.140020441937388	20.401190094082995
36	22.50115526539235	28.501478512063706	25.466146740330487	23.531219482213466
37	22.054528526518745	27.280425592941388	27.783051403049903	22.881994477489968
38	22.094820541187772	25.720532765147635	28.513770991115265	23.670875702549324
39	23.8230520404377	26.131420259371307	26.973227435902693	23.0723002642883
40	20.864206805025347	25.475024641867726	31.66713332559362	21.993635227513302
41	21.931603736003076	26.56666507319716	26.96958521988741	24.532145970912353
42	22.698859303471714	25.2584266082091	29.906918616959523	22.13579547135966
43	22.398717939962623	24.948838246910377	29.847277329709325	22.805166483417675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	14.0
2	20.0
3	83.5
4	147.0
5	147.0
6	194.5
7	242.0
8	237.5
9	233.0
10	370.5
11	508.0
12	508.0
13	811.5
14	1115.0
15	2573.0
16	4031.0
17	4732.5
18	5434.0
19	5434.0
20	5788.5
21	6143.0
22	7745.0
23	9347.0
24	12099.5
25	14852.0
26	14852.0
27	19004.0
28	23156.0
29	28651.5
30	34147.0
31	37799.5
32	41452.0
33	41452.0
34	45036.5
35	48621.0
36	51270.5
37	53920.0
38	57787.0
39	61654.0
40	61654.0
41	63635.5
42	65617.0
43	62967.5
44	60318.0
45	61079.0
46	61840.0
47	61840.0
48	61727.5
49	61615.0
50	74986.5
51	88358.0
52	73026.5
53	57695.0
54	57695.0
55	61158.0
56	64621.0
57	54666.0
58	44711.0
59	33973.5
60	23236.0
61	23236.0
62	20210.0
63	17184.0
64	14095.5
65	11007.0
66	9064.5
67	7122.0
68	7122.0
69	5644.5
70	4167.0
71	3510.5
72	2854.0
73	2217.5
74	1581.0
75	1581.0
76	1194.0
77	807.0
78	649.5
79	492.0
80	336.5
81	181.0
82	181.0
83	132.5
84	84.0
85	61.0
86	38.0
87	28.0
88	18.0
89	18.0
90	13.5
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	878586.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.56512432669061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2343716760525	43.226599710789834
2	11.017478183331212	11.58270220946813
3	2.758795474922735	4.350492813936751
4	1.129973879291662	2.375888698035164
5	0.6069735351104083	1.5952819668044758
6	0.36804004336232776	1.1607642381924814
7	0.2594156499471767	0.9545351124233812
8	0.18119798237151194	0.7619755576883214
9	0.14860259155098446	0.7030182330131328
>10	1.0694367554119646	11.252068646680295
>50	0.13321977789565684	4.910083960450407
>100	0.08642924086870088	8.157313211813214
>500	0.0036824488576512153	1.466109698239351
>1k	0.0012996878321121935	1.3725136923405665
>5k	4.3322927737073124E-4	1.3602164068940108
>10k+	6.498439160560967E-4	4.770435843230494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	16395	1.8660666115781495	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	13030	1.4830648337214571	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	12471	1.4194398727045503	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	6919	0.7875153940536271	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5027	0.5721693721502505	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3444	0.3919934986444128	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2507	0.2853448609470217	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2080	0.23674404099314122	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1647	0.18746030553639598	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1356	0.1543389036474517	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1020	0.11609563548702119	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	991	0.11279487722317451	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	933	0.10619336069548115	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	896	0.10198204842781469	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1381925047747176E-4	0.0
4	0.0	0.0	0.0	1.1381925047747176E-4	0.0
5	0.0	0.0	0.0	1.1381925047747176E-4	0.0
6	0.0	0.0	0.0	1.1381925047747176E-4	0.0
7	0.0	0.0	0.0	1.1381925047747176E-4	0.0
8	0.0	0.0	0.0	1.1381925047747176E-4	0.0
9	0.0	0.0	0.0	2.2763850095494352E-4	0.0
10	1.1381925047747176E-4	0.0	0.0	2.2763850095494352E-4	0.0
11	1.1381925047747176E-4	0.0	0.0	3.4145775143241524E-4	0.0
12	1.1381925047747176E-4	0.0	0.0	4.5527700190988704E-4	0.0
13	1.1381925047747176E-4	0.0	0.0	4.5527700190988704E-4	0.0
14	1.1381925047747176E-4	0.0	0.0	4.5527700190988704E-4	0.0
15	2.2763850095494352E-4	0.0	0.0	4.5527700190988704E-4	0.0
16	2.2763850095494352E-4	0.0	0.0	4.5527700190988704E-4	0.0
17	2.2763850095494352E-4	0.0	0.0	5.690962523873588E-4	0.0
18	2.2763850095494352E-4	0.0	0.0	5.690962523873588E-4	0.0
19	2.2763850095494352E-4	0.0	0.0	7.967347533423023E-4	0.0
20	2.2763850095494352E-4	0.0	0.0	7.967347533423023E-4	0.0
21	2.2763850095494352E-4	0.0	0.0	0.0010243732542972459	0.0
22	2.2763850095494352E-4	0.0	0.0	0.0010243732542972459	0.0
23	2.2763850095494352E-4	0.0	0.0	0.0012520117552521893	0.0
24	2.2763850095494352E-4	0.0	0.0	0.0018211080076395482	0.0
25	2.2763850095494352E-4	0.0	0.0	0.002276385009549435	0.0
26	2.2763850095494352E-4	0.0	0.0	0.0025040235105043785	0.0
27	2.2763850095494352E-4	0.0	0.0	0.003756035265756568	0.0
28	2.2763850095494352E-4	0.0	0.0	0.008308805284855439	0.0
29	2.2763850095494352E-4	0.0	0.0	0.017869622324963065	0.0
30	2.2763850095494352E-4	0.0	0.0	0.03380431739180911	0.0
31	2.2763850095494352E-4	0.0	0.0	0.0732995973074918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	675	0.0	33.437035	37
CTTATAC	1955	0.0	32.079285	1
CGCCGTT	25	0.005495649	29.599998	25
TTATACA	2200	0.0	28.843184	2
GGTATCA	425	0.0	28.72941	1
TATACAC	2300	0.0	27.669565	3
CCGTCTT	2110	0.0	25.251184	37
GCCGTCT	2150	0.0	24.69535	36
GTATCAA	1285	0.0	24.186771	1
TGCCGTC	2460	0.0	21.50813	35
GCACCGC	80	6.95627E-7	20.8125	10
TTAACGG	80	6.95627E-7	20.8125	35
ATGCCGT	2595	0.0	20.38921	34
GGCACCG	85	1.2446253E-6	19.588236	9
TATGCCG	2810	0.0	19.2242	33
TCTATAC	135	5.638867E-11	19.185186	3
CGTCAGA	60	9.234451E-4	18.5	12
AGCCGGT	50	0.007033597	18.499998	31
GCGACTC	50	0.007033597	18.499998	20
GTCGCCA	145	1.546141E-10	17.862068	12
>>END_MODULE