FastQCFastQC Report
Fri 10 Feb 2017
ERR1630880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237180
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT114420.9248452124993938No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG83360.6737903942837744No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT68590.5544059878109895TruSeq Adapter, Index 6 (95% over 21bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA53160.42968686852357785No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC47600.38474595450944893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43430.3510402689988522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40220.32509416576407635No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC24270.19617193941059508RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC24250.19601028144651547No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19040.15389838180377954No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC18690.15106936743238655No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA16250.1313470958146753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13650.11033156048432725No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13230.10693674323865565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC3250.031.30769237
CTTATAC17150.028.909621
TATACAC18200.027.753
TTATACA18550.026.9272232
GGTATCA7750.026.7354831
GATTACG1200.026.2083321
GTATCAA24700.024.9412981
CCGTCTT11300.024.7212437
TTAACGG901.4370016E-1024.66666835
GCCGTCT11750.023.61702236
CGGTAGA400.001931270623.12514
CGGACCG400.001931270623.12535
GCTTTAT11250.021.7066651
TTACGGG1550.021.483873
ATTGAGC11750.019.9957456
TGCCGTC13900.019.69784435
TATTGAG12150.019.4897125
AACGGCC1153.0504452E-919.30434837
TTATTGA12750.019.152944
ATGCCGT14500.019.13793234
Produced by FastQC (version 0.11.5)