FastQCFastQC Report
Fri 10 Feb 2017
ERR1630879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779648
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT153761.9721720571334755No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG131571.68755643572484No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT105621.354713922180266Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC67540.866288376292891No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC44250.5675638236742735Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC31810.4080046379904777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21620.27730462978164505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.2771763667706452No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG16440.2108643900837301No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA12220.15673739944179937No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT10850.13916536693482187No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT10400.13339353143982927No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10280.13185437530783123No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9660.1239020686258414No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8940.11466713183385323No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8700.11158881956985717No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8430.1081257182728616No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT8420.10799745526186176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8220.10543219504186505No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8160.10466261697586604No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8000.10261040879986866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG651.8189894E-1234.1538471
CTTATAC19350.033.940571
CGTCTTC5050.032.2376237
TTATACA20900.031.6004812
CGTTATT303.5984255E-430.83333410
TATACAC22150.030.568853
ATTACGG801.8189894E-1230.0625022
GGTATCA5100.026.8431381
ACGAACG358.866824E-426.42857229
CCGTCTT17950.026.17827437
GCCGTCT18450.025.36856536
AGCAACG551.9016403E-523.5454542
GCAACGC551.9016403E-523.5454543
CGAACGC400.001930632623.12500230
CTAGTAC400.001930632623.1250023
GTATCAA13050.022.3984661
CGCACTC502.701022E-422.19999934
TGCCGTC21300.021.97417835
AGGACCG603.7243066E-521.5833345
TTACGGG1059.804353E-1021.1428573
Produced by FastQC (version 0.11.5)