##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630879.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779648
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285802054260383	31.0	31.0	34.0	28.0	34.0
2	31.284164135609917	31.0	31.0	34.0	28.0	34.0
3	30.717489174601873	31.0	31.0	34.0	26.0	34.0
4	34.92168901863405	37.0	35.0	37.0	32.0	37.0
5	35.10265401822361	37.0	35.0	37.0	32.0	37.0
6	35.30514283368905	37.0	35.0	37.0	33.0	37.0
7	35.3050209838286	37.0	35.0	37.0	33.0	37.0
8	35.34304070554917	37.0	35.0	37.0	33.0	37.0
9	37.08677634009194	39.0	37.0	39.0	33.0	39.0
10	36.901175915284846	39.0	37.0	39.0	33.0	39.0
11	36.96221884747989	39.0	37.0	39.0	33.0	39.0
12	36.89679445082909	39.0	37.0	39.0	33.0	39.0
13	36.98020260425218	39.0	37.0	39.0	33.0	39.0
14	38.0562907876375	40.0	37.0	41.0	33.0	41.0
15	38.07669999794779	40.0	37.0	41.0	33.0	41.0
16	38.07108977384666	40.0	37.0	41.0	33.0	41.0
17	38.022183087752424	40.0	37.0	41.0	33.0	41.0
18	38.06174837875554	40.0	37.0	41.0	33.0	41.0
19	38.09225573592185	40.0	37.0	41.0	33.0	41.0
20	38.11735295928419	40.0	37.0	41.0	33.0	41.0
21	38.058690588573306	40.0	37.0	41.0	33.0	41.0
22	37.995859670004926	40.0	37.0	41.0	33.0	41.0
23	37.98984413478903	40.0	37.0	41.0	33.0	41.0
24	37.92864472172057	40.0	37.0	41.0	33.0	41.0
25	37.902854878098836	40.0	37.0	41.0	33.0	41.0
26	37.74471043342637	40.0	37.0	41.0	32.0	41.0
27	37.7080759522246	40.0	37.0	41.0	32.0	41.0
28	37.6934655126416	40.0	37.0	41.0	32.0	41.0
29	37.634338829830895	39.0	37.0	41.0	32.0	41.0
30	37.577458032342804	39.0	37.0	41.0	32.0	41.0
31	37.535224870710884	39.0	37.0	41.0	32.0	41.0
32	37.44433128796585	39.0	36.0	41.0	32.0	41.0
33	37.38578948448531	39.0	36.0	41.0	32.0	41.0
34	37.311938208011824	39.0	36.0	41.0	31.0	41.0
35	37.31227169184042	39.0	36.0	40.0	31.0	41.0
36	37.26118068666886	39.0	36.0	40.0	31.0	41.0
37	37.1972620977672	39.0	36.0	40.0	31.0	41.0
38	37.162378406665574	39.0	36.0	40.0	31.0	41.0
39	37.065630900098505	39.0	36.0	40.0	31.0	41.0
40	36.98415438762108	39.0	36.0	40.0	31.0	41.0
41	36.948539597356756	39.0	36.0	40.0	31.0	41.0
42	36.896368617632575	39.0	36.0	40.0	31.0	41.0
43	36.160540397717945	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	18.0
11	16.0
12	8.0
13	10.0
14	8.0
15	5.0
16	16.0
17	18.0
18	19.0
19	46.0
20	104.0
21	169.0
22	358.0
23	684.0
24	1190.0
25	1950.0
26	3384.0
27	5026.0
28	7414.0
29	10497.0
30	14412.0
31	18948.0
32	24915.0
33	31913.0
34	41868.0
35	56392.0
36	77901.0
37	115414.0
38	195588.0
39	171343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.70201424232474	22.491175504843213	15.446714414710227	24.360095838121822
2	13.754668773600395	22.234392956821537	41.45768346741093	22.55325480216713
3	14.761789935971104	28.7511800197012	34.89959571498933	21.587434329338368
4	11.723110942373994	19.7635343129207	37.114697914956494	31.398656829748813
5	12.131500369397472	38.92102589886718	35.746388113610244	13.201085618125102
6	27.1875256526022	40.12759604334264	17.89910318502709	14.785775119028074
7	23.784194918732556	33.933518716138565	22.5777530372681	19.70453332786078
8	24.227600147758988	35.75998399277623	22.369197381382367	17.643218478082417
9	23.487137785256937	15.438633845017238	19.26741298637334	41.806815383352486
10	14.048647594812017	29.108264242324744	33.68763852405188	23.155449638811362
11	30.401411919225087	23.375164176654078	25.683898374651125	20.53952552946971
12	20.037632367427353	26.78888421441471	28.986414381874898	24.18706903628304
13	28.63228020850435	20.82760425217534	27.441999466425877	23.098116072894435
14	21.417101050730587	22.950485347233624	26.300073879494335	29.332339722541455
15	24.008783450993267	28.857766581842064	23.072335207683466	24.061114759481203
16	24.732699885076343	27.85988035626334	23.24343806435725	24.163981694303068
17	20.833504350681334	31.10390843047119	24.59494541126252	23.46764180758496
18	23.14685601707437	26.675627975701854	25.797795928418978	24.379720078804795
19	23.497911878180922	28.642926038417336	25.83730093580693	22.021861147594812
20	22.43730504022328	25.64067373994418	26.538386553932032	25.383634665900505
21	23.36605750287309	26.487850927598096	26.615472623542928	23.530618945985882
22	20.885450870136264	30.76606365949762	25.32963593826958	23.018849532096535
23	23.61578558528977	26.026104087998686	26.242355524544408	24.11575480216713
24	23.139160236414384	28.495808364800524	25.84461192743392	22.520419471351175
25	21.751995772451156	28.0458617222131	27.200223690691182	23.001918814644558
26	22.979216261697584	29.394675545887374	26.236968478082417	21.38913971433262
27	20.888657445411262	28.99218621736989	26.363948858972254	23.755207478246593
28	20.846074125759316	26.3289330569693	29.278340994910522	23.546651822360857
29	20.92303193235922	28.278659087177804	26.04931969298966	24.74898928747332
30	22.090610121490727	26.316491544902316	28.27301551469381	23.31988281891315
31	22.048668116893776	26.158343252339517	27.68056866688557	24.112419963881134
32	21.738912945329176	26.35830528648826	28.661908964045313	23.240872804137254
33	22.557615744541128	27.569108110326713	28.48439295682154	21.388883188310622
34	20.78976666393039	28.371008455097684	26.69666310950583	24.1425617714661
35	21.24433077491381	26.12614923657856	32.050232925627974	20.579287062879658
36	22.496306025283204	28.345612378919714	25.369140945657527	23.78894065013955
37	22.355601502216384	27.08414566573633	27.693523230996554	22.866729601050732
38	22.329948900016415	25.3905608684945	28.39063269578066	23.88885753570842
39	23.982746059760302	25.91695226563783	26.849424355606633	23.25087731899524
40	21.019357453620096	25.14724593662781	31.707514160236418	22.12588244951568
41	22.154100311935643	26.50337075192908	26.647153587259893	24.69537534887539
42	23.0497609177475	25.08734711049089	29.74906624527992	22.113825726481696
43	22.768095345591856	24.758609013298308	29.52152766376621	22.95176797734362
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	8.0
2	10.0
3	61.5
4	113.0
5	113.0
6	159.5
7	206.0
8	219.0
9	232.0
10	367.0
11	502.0
12	502.0
13	748.0
14	994.0
15	2526.0
16	4058.0
17	4491.5
18	4925.0
19	4925.0
20	5258.5
21	5592.0
22	7086.5
23	8581.0
24	10893.0
25	13205.0
26	13205.0
27	16275.5
28	19346.0
29	24313.0
30	29280.0
31	31861.0
32	34442.0
33	34442.0
34	37847.0
35	41252.0
36	43134.5
37	45017.0
38	48806.5
39	52596.0
40	52596.0
41	54787.5
42	56979.0
43	54087.5
44	51196.0
45	52320.5
46	53445.0
47	53445.0
48	54800.5
49	56156.0
50	69725.0
51	83294.0
52	68486.0
53	53678.0
54	53678.0
55	56041.5
56	58405.0
57	49661.0
58	40917.0
59	31104.5
60	21292.0
61	21292.0
62	18604.0
63	15916.0
64	13299.0
65	10682.0
66	8786.0
67	6890.0
68	6890.0
69	5610.5
70	4331.0
71	3651.5
72	2972.0
73	2259.0
74	1546.0
75	1546.0
76	1178.5
77	811.0
78	633.0
79	455.0
80	307.0
81	159.0
82	159.0
83	125.0
84	91.0
85	68.0
86	45.0
87	31.5
88	18.0
89	18.0
90	15.5
91	13.0
92	6.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	779648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.76957011466596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12549420618201	40.873505420961685
2	10.728186599413442	10.678744703254543
3	2.8125265063655314	4.1993470547374745
4	1.1667160190116317	2.322678188484134
5	0.6270299048158875	1.5603504405863318
6	0.4343911515537879	1.2971676524668039
7	0.27076313110772426	0.9433035246692664
8	0.22982664041648715	0.9150698475541184
9	0.15968317444984284	0.71526266522225
>10	1.1774892499593468	11.70677829907746
>50	0.15607710740949662	5.445508884544605
>100	0.10353361973602374	9.367575930256287
>500	0.0049178469374419	1.7563399740480556
>1k	0.0023295064440514266	2.3120720151250187
>5k	2.588340493390474E-4	0.8700542896230431
>10k+	7.765021480171421E-4	5.036241109388935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	15376	1.9721720571334755	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	13157	1.68755643572484	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	10562	1.354713922180266	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	6754	0.866288376292891	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	4425	0.5675638236742735	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	3181	0.4080046379904777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2162	0.27730462978164505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.2771763667706452	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1644	0.2108643900837301	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1222	0.15673739944179937	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1085	0.13916536693482187	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1040	0.13339353143982927	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1028	0.13185437530783123	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	966	0.1239020686258414	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	894	0.11466713183385323	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	870	0.11158881956985717	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	843	0.1081257182728616	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	842	0.10799745526186176	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.10543219504186505	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	816	0.10466261697586604	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	800	0.10261040879986866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2826301099983582E-4	0.0
3	0.0	0.0	0.0	1.2826301099983582E-4	0.0
4	0.0	0.0	0.0	1.2826301099983582E-4	0.0
5	0.0	0.0	0.0	1.2826301099983582E-4	0.0
6	0.0	0.0	0.0	1.2826301099983582E-4	0.0
7	0.0	0.0	0.0	1.2826301099983582E-4	0.0
8	0.0	0.0	0.0	1.2826301099983582E-4	0.0
9	0.0	0.0	0.0	2.5652602199967165E-4	0.0
10	0.0	0.0	0.0	2.5652602199967165E-4	0.0
11	1.2826301099983582E-4	0.0	0.0	5.130520439993433E-4	0.0
12	1.2826301099983582E-4	0.0	0.0	5.130520439993433E-4	0.0
13	1.2826301099983582E-4	0.0	0.0	5.130520439993433E-4	0.0
14	1.2826301099983582E-4	0.0	0.0	5.130520439993433E-4	0.0
15	1.2826301099983582E-4	0.0	0.0	6.413150549991791E-4	0.0
16	1.2826301099983582E-4	0.0	0.0	7.69578065999015E-4	0.0
17	1.2826301099983582E-4	0.0	0.0	7.69578065999015E-4	0.0
18	1.2826301099983582E-4	0.0	0.0	8.978410769988508E-4	0.0
19	1.2826301099983582E-4	0.0	0.0	8.978410769988508E-4	0.0
20	1.2826301099983582E-4	0.0	0.0	8.978410769988508E-4	0.0
21	1.2826301099983582E-4	0.0	0.0	0.0010261040879986866	0.0
22	1.2826301099983582E-4	0.0	0.0	0.001410893120998194	0.0
23	1.2826301099983582E-4	0.0	0.0	0.00153915613199803	0.0
24	1.2826301099983582E-4	0.0	0.0	0.0019239451649975374	0.0
25	1.2826301099983582E-4	0.0	0.0	0.002052208175997373	0.0
26	1.2826301099983582E-4	0.0	0.0	0.002180471186997209	0.0
27	1.2826301099983582E-4	0.0	0.0	0.002950049252996224	0.0
28	1.2826301099983582E-4	0.0	0.0	0.008593621736989	0.0
29	1.2826301099983582E-4	0.0	0.0	0.02000902971597439	0.0
30	1.2826301099983582E-4	0.0	0.0	0.03475927598095551	0.0
31	1.2826301099983582E-4	0.0	0.0	0.07503386143490395	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	65	1.8189894E-12	34.153847	1
CTTATAC	1935	0.0	33.94057	1
CGTCTTC	505	0.0	32.23762	37
TTATACA	2090	0.0	31.600481	2
CGTTATT	30	3.5984255E-4	30.833334	10
TATACAC	2215	0.0	30.56885	3
ATTACGG	80	1.8189894E-12	30.062502	2
GGTATCA	510	0.0	26.843138	1
ACGAACG	35	8.866824E-4	26.428572	29
CCGTCTT	1795	0.0	26.178274	37
GCCGTCT	1845	0.0	25.368565	36
AGCAACG	55	1.9016403E-5	23.545454	2
GCAACGC	55	1.9016403E-5	23.545454	3
CGAACGC	40	0.0019306326	23.125002	30
CTAGTAC	40	0.0019306326	23.125002	3
GTATCAA	1305	0.0	22.398466	1
CGCACTC	50	2.701022E-4	22.199999	34
TGCCGTC	2130	0.0	21.974178	35
AGGACCG	60	3.7243066E-5	21.583334	5
TTACGGG	105	9.804353E-10	21.142857	3
>>END_MODULE