FastQCFastQC Report
Fri 10 Feb 2017
ERR1630878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357326
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT172954.840117987496012No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG137163.838511611245753No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT131733.6865495373972226TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC69041.932129204144115No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC56031.5680359111847446RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53891.508146622412027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52911.4807206864319977No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC37151.0396668588347895No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA28400.7947924304416696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20480.5731460906846969No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16540.46288263378539485No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG16100.45056894824334087No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15810.44245311004516885No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA15040.4209041603465743No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14590.4083106183149281No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11920.33358893559382746No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11910.33330907910423535No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT11630.3254730973956555No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11530.3226745324997341No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11340.31735725919748353No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTA11270.3153982637703386No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10510.29412917056133614No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10180.2848939064047956No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9120.2552291185080291No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8370.2342398817886188No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8040.22500461763207824No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7350.20569451985022078No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7280.20373552442307583No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7280.20373552442307583No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7250.20289595495429943No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6740.18862327398510043No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6670.18666427855795548No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6580.18414557015162625No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6440.18022757929733632No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6440.18022757929733632No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6130.17155202811998008No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6090.17043260216161152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.16959303269283513No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5860.16399590290099236No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5700.15951819906751818No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5540.15504049523404398No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5470.15308149980689903No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4980.1393685318168843No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4900.1371296799001472No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4750.13293183255626514No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4700.13153255010830447No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4650.13013326766034378No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4580.1281742722331988No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4570.1278944157436067No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4520.126495133295646No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4480.12537570733727746No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.11893900807665829No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4240.11865915158706616No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4120.1153008737119605No Hit
ATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCA4120.1153008737119605No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4120.1153008737119605No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4070.11390159126399982No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGC4060.11362173477440769No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4060.11362173477440769No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4010.112222452326447No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4010.112222452326447No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3900.10914403094093349No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3870.10830446147215708No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3840.10746489200338066No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3800.10634546604501212No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3780.10578575306582785No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3730.10438647061786716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA200.001840238937.017
GGGCTAT251.2319232E-437.06
CTTATAC18650.035.5120621
ATTACGG603.45608E-1133.9166642
TTATACA20150.032.8684842
GCTATTG352.3837374E-531.7142858
GGCTATT352.3837374E-531.7142857
TTATCAA658.731149E-1131.3076922
TATACAC21450.030.9627043
CGTCTTC6900.030.83333437
TTTAGGG303.5945594E-430.8333322
GTGCAAA303.5945594E-430.83333211
ACAAATA303.5945594E-430.83333222
ATTACTT303.5945594E-430.8333322
GTTTTAG303.5945594E-430.8333321
CTATTGA303.5945594E-430.8333329
TTAGGAC551.8482751E-830.2727283
TTACGGG751.4551915E-1129.63
TAGGACC502.711913E-729.64
GATTACG702.055458E-1029.0714281
Produced by FastQC (version 0.11.5)