FastQCFastQC Report
Fri 10 Feb 2017
ERR1630877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337936
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT155821.164629698281532No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG107850.8060923691417229No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT83000.6203585223807417RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC56880.4251324428074288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34700.259354707549539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.21757393477714929No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC27970.2090533478432451No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC27940.20882912187130026RNA PCR Primer, Index 41 (95% over 23bp)
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC23030.17213080446299375No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21980.16428289544492414No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC19940.14903552935267458No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC19310.1443267839418328No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18310.13685258487700458No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA17640.13184487150356966No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT17520.1309479676157903No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT16990.12698664211143135No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG14890.1112908240752921No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14700.10987072625297473No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA14330.10710527259898829No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC14120.10553569079537435No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT14070.10516198084213296No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA14070.10516198084213296No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA13540.101200655337774No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC13530.10112591334712573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20850.030.4340531
GATTACG1050.029.952381
TTATACA22350.028.8881422
TATACAC22550.028.713973
GGTATCA6900.028.6884041
ACCGTAC358.8701595E-426.4285717
CCGTCTT14400.026.0798637
CGTCTTC4150.025.85542137
GCCGTCT14450.025.60553736
GTATCAA19700.023.383251
TGCCGTC17350.021.43227835
GCAACGC450.003826131620.5555553
ATGCCGT18550.020.04582234
TTACGGG1403.6379788E-1219.8214283
TACACAT35050.018.8430825
ATACACA35200.018.7627834
TCGCCGA500.007035470618.51
CGGAATT701.2193871E-418.527
ATTACGG1501.2732926E-1118.52
AGAACCG500.007035470618.55
Produced by FastQC (version 0.11.5)