##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630877.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337936
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.542672444720825	31.0	31.0	34.0	30.0	34.0
2	31.5348873189749	31.0	31.0	34.0	30.0	34.0
3	31.00117045957355	31.0	31.0	34.0	27.0	34.0
4	35.15406267564368	37.0	35.0	37.0	32.0	37.0
5	35.35307368962342	37.0	35.0	37.0	33.0	37.0
6	35.53853547553844	37.0	35.0	37.0	33.0	37.0
7	35.552596686239106	37.0	35.0	37.0	33.0	37.0
8	35.567705032228744	37.0	35.0	37.0	33.0	37.0
9	37.337334521232705	39.0	37.0	39.0	34.0	39.0
10	37.18938947752359	39.0	37.0	39.0	33.0	39.0
11	37.23906225708853	39.0	37.0	39.0	34.0	39.0
12	37.19338518434365	39.0	37.0	39.0	33.0	39.0
13	37.2454228004927	39.0	37.0	39.0	34.0	39.0
14	38.41873153872831	40.0	38.0	41.0	34.0	41.0
15	38.43213800959089	40.0	38.0	41.0	34.0	41.0
16	38.42322353236627	40.0	38.0	41.0	34.0	41.0
17	38.41023860633094	40.0	38.0	41.0	34.0	41.0
18	38.43574505805958	40.0	38.0	41.0	34.0	41.0
19	38.47467218162902	40.0	38.0	41.0	34.0	41.0
20	38.46995745685892	40.0	38.0	41.0	34.0	41.0
21	38.42622517071071	40.0	38.0	41.0	34.0	41.0
22	38.37512257686466	40.0	38.0	41.0	34.0	41.0
23	38.36080500113608	40.0	38.0	41.0	34.0	41.0
24	38.32868537807489	40.0	38.0	41.0	34.0	41.0
25	38.30188514248813	40.0	38.0	41.0	34.0	41.0
26	38.201394536061514	40.0	38.0	41.0	34.0	41.0
27	38.15432352519104	40.0	38.0	41.0	33.0	41.0
28	38.133401747168776	40.0	38.0	41.0	33.0	41.0
29	38.074781603903325	40.0	37.0	41.0	33.0	41.0
30	38.003498672582246	40.0	37.0	41.0	33.0	41.0
31	37.9858767534471	40.0	37.0	41.0	33.0	41.0
32	37.90303347843245	40.0	37.0	41.0	33.0	41.0
33	37.8860446239581	40.0	37.0	41.0	33.0	41.0
34	37.82931694789586	40.0	37.0	41.0	33.0	41.0
35	37.79772201360902	40.0	37.0	41.0	33.0	41.0
36	37.77688170435656	40.0	37.0	41.0	33.0	41.0
37	37.73728862965044	40.0	37.0	41.0	33.0	41.0
38	37.69038653567884	40.0	37.0	41.0	33.0	41.0
39	37.62457696033293	40.0	37.0	41.0	32.0	41.0
40	37.578239168390716	40.0	37.0	41.0	32.0	41.0
41	37.52835486899224	40.0	37.0	41.0	32.0	41.0
42	37.47821794166537	40.0	37.0	41.0	32.0	41.0
43	36.85937369201516	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	12.0
11	21.0
12	9.0
13	8.0
14	4.0
15	13.0
16	9.0
17	12.0
18	14.0
19	55.0
20	93.0
21	197.0
22	392.0
23	755.0
24	1365.0
25	2450.0
26	4018.0
27	6531.0
28	9709.0
29	14299.0
30	19946.0
31	26939.0
32	36031.0
33	46995.0
34	62558.0
35	84906.0
36	119673.0
37	178285.0
38	318980.0
39	403647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50502864113082	21.621587280707	14.81468470838665	24.058699369775535
2	13.986468710013037	22.467517130864255	40.91817545831788	22.627838700804823
3	14.82455065115222	28.108893101015298	34.57437426005429	22.49218198777819
4	12.189671254828333	19.02669484937994	38.63697516174167	30.146658734050057
5	12.329289293359324	38.741987658602504	36.077360950000596	12.851362098037574
6	27.696989990552613	40.219935781681635	18.15953827387857	13.923535953887182
7	24.315288623671087	33.458999533609976	23.53640233912534	18.689309503593595
8	24.023495892180193	36.288208105619404	21.941408258691	17.746887743509408
9	24.44182681383863	15.928265627055405	20.030330299805073	39.599577259300894
10	14.380209516748184	29.21858743617034	34.163293311488744	22.237909735592734
11	31.115613900814388	23.84314346874589	25.740693127324477	19.300549503115246
12	20.77064971717631	26.563079250427524	29.94156671171117	22.724704320684996
13	27.64227885339807	21.636012484902118	27.78929634900324	22.93241231269657
14	21.422997811554513	22.242693222994223	27.290169335454014	29.04413962999725
15	23.403884789705934	29.157224261848103	24.105338371940064	23.3335525765059
16	23.791496753207927	28.430208918812262	24.160497961038494	23.617796366941317
17	21.52188146518219	29.834835149065427	25.629626529221127	23.013656856531252
18	22.80116537711819	27.249135982588108	26.813464919099268	23.136233721194436
19	23.830811040288925	27.156530656174883	26.96967567955418	22.042982623982013
20	23.23227717917748	26.114178854593938	27.38277466186723	23.270769304361345
21	22.716407959723036	27.315207902321188	26.71338539362122	23.254998744334557
22	21.0625919326485	30.476644622762223	26.354698580500113	22.106064864089163
23	22.191195991437557	27.62008048217553	26.991724566795423	23.19699895959149
24	23.928723048038172	28.077127754989778	26.11305772473422	21.881091472237834
25	22.321022829193623	28.47490462921993	26.585726073593953	22.618346467992488
26	23.299320744788986	26.771908372298824	26.5967878882099	23.331982994702287
27	21.62465170232358	28.94144413484651	26.636176917281546	22.797727245548366
28	20.878053957737887	27.428890470097222	28.394631731263676	23.298423840901208
29	22.412058573803233	28.199330909699714	26.187052295476015	23.201558221021035
30	21.232256251420097	28.73814592028318	27.096438095693664	22.933159732603052
31	23.348725200607504	26.59424666052786	27.68450807811435	22.37252006075029
32	21.66194795565707	29.290115521220745	27.20600985398405	21.841926669138132
33	21.47621410889609	27.81313904402004	27.945731335430096	22.764915511653772
34	22.571632723837315	27.999844536659452	26.855096207890362	22.573426531612874
35	21.811058227000395	28.27982803362792	28.360474641537415	21.548639097834275
36	22.645627294579114	27.023788880783535	25.928594491814255	24.401989332823096
37	22.909092811614308	27.045389316080886	27.272754451632963	22.77276342067184
38	22.809013286136256	26.446332261034904	27.828236926130995	22.91641752669784
39	23.18922579256407	26.47593008933163	27.222677317898615	23.112166800205692
40	21.916668659786417	26.085328446203704	29.617410698269573	22.380592195740302
41	22.143136891450713	26.72033639875151	27.262963250858036	23.87356345893974
42	23.075468482797383	26.060140395355237	28.31443357529807	22.54995754654931
43	22.55616113177312	25.758556463089416	28.746591765226437	22.938690639911027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	21.5
2	31.0
3	101.5
4	172.0
5	172.0
6	249.0
7	326.0
8	323.0
9	320.0
10	517.5
11	715.0
12	715.0
13	1086.0
14	1457.0
15	3734.5
16	6012.0
17	7078.5
18	8145.0
19	8145.0
20	8808.5
21	9472.0
22	12211.0
23	14950.0
24	18834.0
25	22718.0
26	22718.0
27	28281.5
28	33845.0
29	42875.0
30	51905.0
31	55779.0
32	59653.0
33	59653.0
34	65277.5
35	70902.0
36	75363.0
37	79824.0
38	87778.5
39	95733.0
40	95733.0
41	101113.5
42	106494.0
43	100188.5
44	93883.0
45	96115.0
46	98347.0
47	98347.0
48	98994.5
49	99642.0
50	112144.5
51	124647.0
52	111809.0
53	98971.0
54	98971.0
55	92189.0
56	85407.0
57	72884.0
58	60361.0
59	48703.5
60	37046.0
61	37046.0
62	32176.0
63	27306.0
64	23132.5
65	18959.0
66	15719.0
67	12479.0
68	12479.0
69	10013.5
70	7548.0
71	6336.0
72	5124.0
73	3972.0
74	2820.0
75	2820.0
76	2122.0
77	1424.0
78	1093.5
79	763.0
80	510.0
81	257.0
82	257.0
83	197.5
84	138.0
85	109.5
86	81.0
87	57.5
88	34.0
89	34.0
90	22.0
91	10.0
92	5.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1337936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.72091202313737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24395661487227	34.208971435010085
2	12.55242640902681	10.976070614119346
3	3.650081819244465	4.787547182893214
4	1.6129770997324502	2.8208331949095093
5	0.8398322228484884	1.8359115364677332
6	0.5157188493180553	1.3528619063825
7	0.3666030299336106	1.121975317340009
8	0.26234788543091675	0.9176071054704988
9	0.19379384206321698	0.762555916752452
>10	1.4169905282254986	12.309112983927221
>50	0.17773893981749206	5.41394364007862
>100	0.14964425364119743	13.178566604985479
>500	0.01221234533687122	3.599021873089439
>1k	0.004988141053088244	3.6802401359281824
>5k	3.4400972779918923E-4	1.0519269079521725
>10k+	3.4400972779918923E-4	1.9828536446936613
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	15582	1.164629698281532	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	10785	0.8060923691417229	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	8300	0.6203585223807417	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	5688	0.4251324428074288	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3470	0.259354707549539	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2911	0.21757393477714929	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2797	0.2090533478432451	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	2794	0.20882912187130026	RNA PCR Primer, Index 41 (95% over 23bp)
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2303	0.17213080446299375	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2198	0.16428289544492414	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1994	0.14903552935267458	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1931	0.1443267839418328	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1831	0.13685258487700458	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1764	0.13184487150356966	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1752	0.1309479676157903	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1699	0.12698664211143135	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1489	0.1112908240752921	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1470	0.10987072625297473	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1433	0.10710527259898829	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1412	0.10553569079537435	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1407	0.10516198084213296	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1407	0.10516198084213296	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1354	0.101200655337774	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1353	0.10112591334712573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.474199064828212E-5	0.0
10	0.0	0.0	0.0	1.4948398129656425E-4	0.0
11	0.0	0.0	0.0	1.4948398129656425E-4	0.0
12	7.474199064828212E-5	0.0	0.0	2.989679625931285E-4	0.0
13	7.474199064828212E-5	0.0	0.0	2.989679625931285E-4	0.0
14	7.474199064828212E-5	0.0	0.0	2.989679625931285E-4	0.0
15	7.474199064828212E-5	0.0	0.0	2.989679625931285E-4	0.0
16	7.474199064828212E-5	0.0	0.0	4.484519438896928E-4	0.0
17	7.474199064828212E-5	0.0	0.0	4.484519438896928E-4	0.0
18	7.474199064828212E-5	0.0	0.0	4.484519438896928E-4	0.0
19	7.474199064828212E-5	0.0	0.0	6.726779158345392E-4	0.0
20	7.474199064828212E-5	0.0	0.0	6.726779158345392E-4	0.0
21	7.474199064828212E-5	0.0	0.0	9.716458784276677E-4	0.0
22	7.474199064828212E-5	0.0	0.0	0.0014200978223173604	0.0
23	7.474199064828212E-5	0.0	0.0	0.0016443237942622068	0.0
24	7.474199064828212E-5	0.0	0.0	0.002317001710096746	0.0
25	7.474199064828212E-5	0.0	0.0	0.002391743700745028	0.0
26	7.474199064828212E-5	0.0	0.0	0.0024664856913933103	0.0
27	7.474199064828212E-5	7.474199064828212E-5	0.0	0.002914937635283003	0.0
28	7.474199064828212E-5	7.474199064828212E-5	0.0	0.009641716793628395	0.0
29	7.474199064828212E-5	7.474199064828212E-5	0.0	0.021301467334760408	0.0
30	7.474199064828212E-5	7.474199064828212E-5	0.0	0.03826789921192045	0.0
31	7.474199064828212E-5	7.474199064828212E-5	0.0	0.08864400090886261	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2085	0.0	30.434053	1
GATTACG	105	0.0	29.95238	1
TTATACA	2235	0.0	28.888142	2
TATACAC	2255	0.0	28.71397	3
GGTATCA	690	0.0	28.688404	1
ACCGTAC	35	8.8701595E-4	26.42857	17
CCGTCTT	1440	0.0	26.07986	37
CGTCTTC	415	0.0	25.855421	37
GCCGTCT	1445	0.0	25.605537	36
GTATCAA	1970	0.0	23.38325	1
TGCCGTC	1735	0.0	21.432278	35
GCAACGC	45	0.0038261316	20.555555	3
ATGCCGT	1855	0.0	20.045822	34
TTACGGG	140	3.6379788E-12	19.821428	3
TACACAT	3505	0.0	18.843082	5
ATACACA	3520	0.0	18.762783	4
TCGCCGA	50	0.0070354706	18.5	1
CGGAATT	70	1.2193871E-4	18.5	27
ATTACGG	150	1.2732926E-11	18.5	2
AGAACCG	50	0.0070354706	18.5	5
>>END_MODULE