FastQCFastQC Report
Fri 10 Feb 2017
ERR1630876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295197
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT186206.307652178037039No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG144944.909941496695428No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT124944.2324278363262495RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC74232.514591950460201No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC53551.814042825638472RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42441.4376839873033942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41931.42040738896398No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC41331.4000819791529047No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27650.9366626354603875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16330.5531899036914332No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG16240.5501410922197719No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15860.5372683326727575No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA15390.5213467616540819No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15340.519652977503159No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12840.4349637699570118No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT12540.4248010650514741No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12040.4078632235422447No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11960.40515316890076797No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11590.39261916618393816No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11260.38144019078784674No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10980.37195499954267824No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9950.33706304603366566No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9470.3208027181848054No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9160.3103012564490832No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7880.26694038218545585No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7410.25101881116678015No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7260.24593745871401132No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7170.24288864724235004No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7050.23882356528013496No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6990.2367910242990274No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6760.2289996172047819No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6530.22120821011053635No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6280.21273928935592162No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6260.21206177569555248No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5700.1930913932052155No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5610.1900425817335542No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5470.18529998611096996No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5410.18326744512986243No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5150.17445976754506312No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5090.1724272265639556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.16429706263952548No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4800.1626032784886025No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4630.1568444123754645No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4580.1551506282245416No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4530.15345684407361862No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4400.14905300528121898No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4370.1480367347906652No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4350.14735922113029604No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4250.14397165282845015No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4220.14295538233789637No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4180.14160035501715804No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4160.14092284135678884No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4100.1388903003756813No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4100.1388903003756813No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4020.13618024573420462No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3840.13008262279088204No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3810.12906635230032826No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3780.12805008180977448No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3620.12262997252682106No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3600.12195245886645188No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3550.12025867471552895No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3540.11991991788534435No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3420.11585483592312931No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3410.1155160790929447No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3330.11280602445146799No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.11212851079109881No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3290.11145099713072965No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3290.11145099713072965No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3220.10907969931943752No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3170.10738591516851459No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3170.10738591516851459No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3150.10670840150814541No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3110.10535337418740706No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3040.10298207637611492No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3040.10298207637611492No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3020.10230456271574574No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2980.10094953539500741No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2980.10094953539500741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2960.10027202173463823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG200.00183972137.01
TTCTACA200.00183972137.02
TAGCGAA200.00183972137.010
TTGTATC200.00183972137.02
TGTATCC200.00183972137.03
TCTACAC200.00183972137.03
GTTGTAT200.00183972137.01
TCAGAGT355.612419E-737.03
CTTCTAC200.00183972137.01
GTATACA251.2314037E-437.02
TTACGGG452.6066118E-937.03
CTTATAC19800.035.4116171
CGTCTTC6350.034.9606337
TTATACA20900.033.6363642
ATTACGG507.2923285E-933.3000032
CTCAGAG451.07535016E-732.888892
ACTCAGA401.5947389E-632.3751
TATACAC22250.032.0112343
TTTACAC352.3823424E-531.7142852
GATACAC601.2623786E-930.8333343
Produced by FastQC (version 0.11.5)