FastQCFastQC Report
Fri 10 Feb 2017
ERR1630875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839622
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT100311.194704283594284No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG77480.9227962106757563No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT62070.7392612389861152TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC45390.5406004130430122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23590.2809597652276858No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22960.27345638870825206No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC22420.26702492312016596Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC22190.2642855951844997No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21670.258092332025602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.24332378141592287No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19770.23546310125270656No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC19050.22688781380192516No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT18970.22593500408517167No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC18960.22581590287057748No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG16800.2000900405182332No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT16290.19401587857392968No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA14700.17507878545345407No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA14450.17210125508859941No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA14310.17043383808428078No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG13580.16173944941890517No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC13330.15876191905405052No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT13230.15757090690810865No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13200.1572136032643261No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA12110.14423157087355978No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG11250.13398886641845972No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT10790.12851021054712716No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC10540.12553268018227248No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG10440.12434166803633062No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA10040.11957761945256316No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG9990.11898211337959225No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA9990.11898211337959225No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG9590.11421806479582479No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC9370.1115978380747527No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC8920.10623828341801429No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG8680.10337985426775381No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG8500.10123603240505846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13200.031.1136361
TTATACA14250.028.5614032
TATACAC15050.028.0265793
GATTACG405.9382506E-527.7500021
GGTATCA4700.027.5531921
CGTCTTC3500.027.48571637
CCGTCTT10200.025.75490237
GCCGTCT10550.024.90047336
GTATCAA12600.022.904761
TTACGGG502.7012618E-422.23
GTCGCCA3000.020.96666512
CGAACTA450.003824961620.55555729
TCGCCAT3150.019.96825413
TGCCGTC13250.019.96603835
TATAGAG851.2444907E-619.5882365
ATGCCGT13850.018.83393534
ATTGTAC701.2187009E-418.53
GCACCGC500.007033341618.525
CCATCCG500.007033341618.59
GGTCTAT701.2187009E-418.51
Produced by FastQC (version 0.11.5)