##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839622
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.517915204699257	31.0	31.0	34.0	28.0	34.0
2	31.525300671016243	31.0	31.0	34.0	30.0	34.0
3	30.979848074490665	31.0	31.0	34.0	27.0	34.0
4	35.13592068811918	37.0	35.0	37.0	32.0	37.0
5	35.33325353551955	37.0	35.0	37.0	33.0	37.0
6	35.5209391845378	37.0	35.0	37.0	33.0	37.0
7	35.53535876858872	37.0	35.0	37.0	33.0	37.0
8	35.55381826583867	37.0	35.0	37.0	33.0	37.0
9	37.324874765072856	39.0	37.0	39.0	34.0	39.0
10	37.170697051768535	39.0	37.0	39.0	33.0	39.0
11	37.218961627970685	39.0	37.0	39.0	33.0	39.0
12	37.16255410172673	39.0	37.0	39.0	33.0	39.0
13	37.21993111185748	39.0	37.0	39.0	34.0	39.0
14	38.376375321275525	40.0	38.0	41.0	34.0	41.0
15	38.408471907596514	40.0	38.0	41.0	34.0	41.0
16	38.38216959536553	40.0	38.0	41.0	34.0	41.0
17	38.38098453828032	40.0	38.0	41.0	34.0	41.0
18	38.397873090509776	40.0	38.0	41.0	34.0	41.0
19	38.44626034096296	40.0	38.0	41.0	34.0	41.0
20	38.44834342120621	40.0	38.0	41.0	34.0	41.0
21	38.39456684079264	40.0	38.0	41.0	34.0	41.0
22	38.35811591406609	40.0	38.0	41.0	34.0	41.0
23	38.33392169333343	40.0	38.0	41.0	34.0	41.0
24	38.30026726312555	40.0	38.0	41.0	34.0	41.0
25	38.2752178956721	40.0	38.0	41.0	34.0	41.0
26	38.16565549735476	40.0	38.0	41.0	33.0	41.0
27	38.11445507621287	40.0	38.0	41.0	33.0	41.0
28	38.09797980519805	40.0	38.0	41.0	33.0	41.0
29	38.048473003327686	40.0	37.0	41.0	33.0	41.0
30	37.98165007586747	40.0	37.0	41.0	33.0	41.0
31	37.97331656388232	40.0	37.0	41.0	33.0	41.0
32	37.87053697973612	40.0	37.0	41.0	33.0	41.0
33	37.84946440183797	40.0	37.0	41.0	33.0	41.0
34	37.78895502976339	40.0	37.0	41.0	33.0	41.0
35	37.753302081174624	40.0	37.0	41.0	33.0	41.0
36	37.74496023210445	40.0	37.0	41.0	33.0	41.0
37	37.67894957492776	40.0	37.0	41.0	33.0	41.0
38	37.63696996981975	40.0	37.0	41.0	33.0	41.0
39	37.58055767952722	40.0	37.0	41.0	32.0	41.0
40	37.532488429317	40.0	37.0	41.0	32.0	41.0
41	37.4948488724688	40.0	37.0	41.0	32.0	41.0
42	37.453445717239426	40.0	37.0	41.0	32.0	41.0
43	36.81425927381608	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	0.0
9	8.0
10	7.0
11	8.0
12	5.0
13	4.0
14	6.0
15	2.0
16	3.0
17	8.0
18	13.0
19	31.0
20	57.0
21	121.0
22	234.0
23	488.0
24	878.0
25	1650.0
26	2629.0
27	4133.0
28	6166.0
29	9154.0
30	12718.0
31	17181.0
32	22735.0
33	29947.0
34	39723.0
35	53520.0
36	75433.0
37	113740.0
38	202942.0
39	246075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.49208334226593	22.70855218181515	15.146458763586471	23.652905712332455
2	13.561578900981633	22.974743396433155	41.56953962616511	21.894138076420102
3	14.684107848531838	28.286181162475494	35.6612856737913	21.368425315201364
4	12.109139589005528	19.176248359380768	38.3964450669468	30.31816698466691
5	11.914409103144035	39.499917820161926	36.01847021635927	12.56720286033477
6	27.137807251358346	40.41973650047283	18.36862302321759	14.07383322495123
7	23.37087403617342	34.331877916490996	23.57989666778622	18.717351379549367
8	23.612649501799616	36.91577876711187	22.11114048941071	17.360431241677805
9	23.64849896739247	16.25136073137674	20.148114270469332	39.95202603076146
10	13.877554423300008	29.858793599977133	34.334498143212066	21.929153833510796
11	30.64724364059065	24.07988356665261	26.156413243102257	19.11645954965449
12	20.018413047776264	27.14149938901077	30.184535421892235	22.655552141320737
13	27.1264926359719	21.92712911286269	28.409570020795073	22.536808230370333
14	20.70229222197608	23.020716465266513	27.63565032836205	28.64134098439536
15	23.158278368122797	29.573546191024057	24.599164862283267	22.669010578569882
16	23.36086953414751	29.165862733468156	24.226378060603462	23.246889671780874
17	21.129150975081643	30.492888466476582	25.74265562360205	22.635304934839727
18	21.834587469123008	27.889335915447667	27.330870320215528	22.9452062952138
19	23.367658303379375	28.67278370504822	27.482367065179332	20.477190926393067
20	22.689376886265485	26.357813396981022	28.0299944498834	22.922815266870092
21	22.378284513745474	27.927448304117807	27.042049874824624	22.6522173073121
22	20.894521582331098	30.914030361281625	26.494541591335146	21.69690646505213
23	21.761935728220557	28.427316101769605	27.11672633637518	22.694021833634658
24	24.33726129139065	28.651226385206673	26.27575266012563	20.735759663277044
25	22.23738777688055	27.998194425586753	26.782528328223894	22.981889469308808
26	23.651476497757322	27.848245996412675	26.98595320275076	21.514324303079242
27	21.556247930616397	28.730428692911808	27.160674684560433	22.552648691911358
28	20.236487371698217	28.060127057175727	28.977444611980154	22.725940959145902
29	22.880891639332937	29.11965146220561	26.5529011864863	21.44655571197515
30	20.478262837324415	28.94099964031433	27.56395139717635	23.016786125184904
31	23.604193315563432	26.946768903149277	28.037259623973643	21.41177815731365
32	22.469396942910024	27.77869088708967	27.544180595553712	22.207731574446594
33	21.912360562253014	27.97199215837603	28.718875875096174	21.39677140427478
34	21.779324505551305	28.623833105850018	27.11160498414763	22.48523740445105
35	21.49550631117336	27.285969162313517	28.96910752695856	22.249416999554562
36	23.384808878280943	27.66185259557277	26.060298562924743	22.893039963221547
37	22.860525331637334	27.170440984157157	27.52857833644187	22.440455347763635
38	22.299320408469526	26.909132919337512	28.405163275855088	22.386383396337877
39	22.98736812518014	26.92687900031204	27.68865036885646	22.397102505651354
40	21.487645631010142	26.138548060913124	30.30018270126319	22.07362360681354
41	21.569229963007164	27.590868271674633	27.58169747815088	23.25820428716732
42	22.73868478910748	26.083999704628987	29.070224458149024	22.10709104811451
43	21.891875153342813	26.233829032588474	29.1178649439867	22.756430870082013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	13.0
2	17.0
3	70.5
4	124.0
5	124.0
6	175.5
7	227.0
8	229.0
9	231.0
10	389.5
11	548.0
12	548.0
13	799.5
14	1051.0
15	2806.0
16	4561.0
17	5247.0
18	5933.0
19	5933.0
20	6424.5
21	6916.0
22	9058.0
23	11200.0
24	13907.5
25	16615.0
26	16615.0
27	20174.0
28	23733.0
29	31057.5
30	38382.0
31	39603.5
32	40825.0
33	40825.0
34	44247.0
35	47669.0
36	49488.5
37	51308.0
38	56496.5
39	61685.0
40	61685.0
41	65843.0
42	70001.0
43	62869.0
44	55737.0
45	57264.0
46	58791.0
47	58791.0
48	59073.0
49	59355.0
50	60385.0
51	61415.0
52	70299.0
53	79183.0
54	79183.0
55	59058.0
56	38933.0
57	39630.0
58	40327.0
59	33109.0
60	25891.0
61	25891.0
62	20052.5
63	14214.0
64	11703.5
65	9193.0
66	7682.0
67	6171.0
68	6171.0
69	5046.0
70	3921.0
71	3244.0
72	2567.0
73	1983.0
74	1399.0
75	1399.0
76	1080.0
77	761.0
78	592.0
79	423.0
80	286.0
81	149.0
82	149.0
83	116.0
84	83.0
85	65.0
86	47.0
87	31.5
88	16.0
89	16.0
90	13.0
91	10.0
92	5.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	839622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.7487787268701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81036618305626	36.16165195162931
2	11.482574572220026	10.27662377494118
3	3.1911229003051966	4.283965576680156
4	1.3021255665785758	2.330741154137002
5	0.6812536077070789	1.524263347408302
6	0.4333897133670793	1.1636196231579052
7	0.2927677183094559	0.9170698491500335
8	0.21817693038332628	0.7810520944824968
9	0.16289490337989068	0.6560413188373451
>10	1.1113982322372211	9.913573882799637
>50	0.13595596570089813	4.292476201562842
>100	0.15576044711492987	15.014196148318906
>500	0.014452000247751846	4.417384220297661
>1k	0.006958370489658298	5.394753682730879
>5k	5.352592684352536E-4	1.671264654853026
>10k+	2.676296342176268E-4	1.2013225190133074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	10031	1.194704283594284	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	7748	0.9227962106757563	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	6207	0.7392612389861152	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	4539	0.5406004130430122	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.2809597652276858	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2296	0.27345638870825206	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2242	0.26702492312016596	Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	2219	0.2642855951844997	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2167	0.258092332025602	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2043	0.24332378141592287	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1977	0.23546310125270656	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1905	0.22688781380192516	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1897	0.22593500408517167	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1896	0.22581590287057748	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1680	0.2000900405182332	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1629	0.19401587857392968	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1470	0.17507878545345407	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1445	0.17210125508859941	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1431	0.17043383808428078	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1358	0.16173944941890517	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1333	0.15876191905405052	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1323	0.15757090690810865	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1320	0.1572136032643261	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1211	0.14423157087355978	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1125	0.13398886641845972	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1079	0.12851021054712716	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1054	0.12553268018227248	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1044	0.12434166803633062	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1004	0.11957761945256316	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	999	0.11898211337959225	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	999	0.11898211337959225	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	959	0.11421806479582479	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	937	0.1115978380747527	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	892	0.10623828341801429	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	868	0.10337985426775381	No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG	850	0.10123603240505846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1910121459418643E-4	0.0
10	0.0	0.0	0.0	1.1910121459418643E-4	0.0
11	0.0	0.0	0.0	1.1910121459418643E-4	0.0
12	0.0	0.0	0.0	1.1910121459418643E-4	0.0
13	0.0	0.0	0.0	1.1910121459418643E-4	0.0
14	0.0	0.0	0.0	1.1910121459418643E-4	0.0
15	0.0	0.0	0.0	3.573036437825593E-4	0.0
16	0.0	0.0	0.0	4.764048583767457E-4	0.0
17	0.0	0.0	0.0	5.955060729709321E-4	0.0
18	0.0	0.0	0.0	5.955060729709321E-4	0.0
19	0.0	0.0	0.0	8.33708502159305E-4	0.0
20	0.0	0.0	0.0	9.528097167534914E-4	0.0
21	0.0	0.0	0.0	0.0013101133605360508	0.0
22	0.0	0.0	0.0	0.001429214575130237	0.0
23	0.0	0.0	0.0	0.0017865182189127965	0.0
24	0.0	0.0	0.0	0.002143821862695356	0.0
25	0.0	0.0	0.0	0.002143821862695356	0.0
26	0.0	0.0	0.0	0.0023820242918837285	0.0
27	0.0	0.0	0.0	0.003215732794043034	0.0
28	0.0	0.0	0.0	0.008575287450781423	0.0
29	0.0	0.0	0.0	0.019770801622634948	0.0
30	0.0	0.0	0.0	0.03704047773879198	0.0
31	0.0	0.0	0.0	0.08932591094563982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1320	0.0	31.113636	1
TTATACA	1425	0.0	28.561403	2
TATACAC	1505	0.0	28.026579	3
GATTACG	40	5.9382506E-5	27.750002	1
GGTATCA	470	0.0	27.553192	1
CGTCTTC	350	0.0	27.485716	37
CCGTCTT	1020	0.0	25.754902	37
GCCGTCT	1055	0.0	24.900473	36
GTATCAA	1260	0.0	22.90476	1
TTACGGG	50	2.7012618E-4	22.2	3
GTCGCCA	300	0.0	20.966665	12
CGAACTA	45	0.0038249616	20.555557	29
TCGCCAT	315	0.0	19.968254	13
TGCCGTC	1325	0.0	19.966038	35
TATAGAG	85	1.2444907E-6	19.588236	5
ATGCCGT	1385	0.0	18.833935	34
ATTGTAC	70	1.2187009E-4	18.5	3
GCACCGC	50	0.0070333416	18.5	25
CCATCCG	50	0.0070333416	18.5	9
GGTCTAT	70	1.2187009E-4	18.5	1
>>END_MODULE